The DOI matches its Crossref record.
Integration and global analysis of isothermal titration calorimetry data for studying macromolecular interactions
Open accessAbstract
Isothermal titration calorimetry (ITC) is a powerful and widely used method to measure the energetics of macromolecular interactions by recording a thermogram of differential heating power during a titration. However, traditional ITC analysis is limited by stochastic thermogram noise and by the limited information content of a single titration experiment. Here we present a protocol for bias-free thermogram integration based on automated shape analysis of the injection peaks, followed by combination of isotherms from different calorimetric titration experiments into a global analysis, statistical analysis of binding parameters and graphical presentation of the results. This is performed using the integrated public-domain software packages NITPIC, SEDPHAT and GUSSI. The recently developed low-noise thermogram integration approach and global analysis allow for more precise parameter estimates and more reliable quantification of multisite and multicomponent cooperative and competitive interactions. Titration experiments typically take 1–2.5 h each, and global analysis usually takes 10–20 min.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Li, T., Wang, P., Guo, W., Huang, X., Tian, X., Wu, G., Xu, B., Li, F., Yan, C., Liang, X.-J., et al. (2019) · ACS Nano · doi:10.1021/acsnano.9b01346 · Cited by 462
The Fab region of IgG impairs the internalization pathway of FcRn upon Fc engagement
Brinkhaus, M., Pannecoucke, E., van der Kooi, E. J., Bentlage, A. E. H., Derksen, N. I. L., Andries, J., Balbino, B., Sips, M., Ulrichts, P., Verheesen, P., et al. (2022) · Nature Communications · doi:10.1038/s41467-022-33764-1 · Cited by 51
Structure and mechanism of a tripartite ATP-independent periplasmic TRAP transporter
Davies, J. S., Currie, M. J., North, R. A., Scalise, M., Wright, J. D., Copping, J. M., Remus, D. M., Gulati, A., Morado, D. R., Jamieson, S. A., et al. (2023) · Nature Communications · doi:10.1038/s41467-023-36590-1 · Cited by 34
Manna, A., Zhao, H., Wada, J., Balagopalan, L., Tagad, H. D., Appella, E., Schuck, P., & Samelson, L. E. (2018) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1817142115 · Cited by 24
Self-citation(2022) · doi:10.1093/nar/gkac563 · Cited by 22
Metadata
- Authors
- Brautigam, C. A., Zhao, H., Vargas, C., Keller, S., & Schuck, P.
- Venue
- Nature Protocols
- Published
- 2016-04-07
- Volume
- 11
- Issue
- 5
- Pages
- 882-894
- ISSN
- 1750-2799 (ISSN-L)
- 1754-2189
- OpenAlex
- W2321635162
- Cited by
- 276
- References
- 0
- FWCI
- 17.83
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Integration and global analysis of isothermal titration calorimetry data for studying macromolecular interactions. https://app.cochat.ai/papers/10.1038/nprot.2016.044
- The paper
- Brautigam, C. A., Zhao, H., Vargas, C., Keller, S., & Schuck, P. (2016). Integration and global analysis of isothermal titration calorimetry data for studying macromolecular interactions. Nature Protocols. https://doi.org/10.1038/nprot.2016.044
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
