The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Structure determination of the seven-helix transmembrane receptor sensory rhodopsin II by solution NMR spectroscopy
Open accessAbstract
Seven-helix membrane proteins represent a challenge for structural biology. Here we report the first NMR structure determination of a detergent-solubilized seven-helix transmembrane (7TM) protein, the phototaxis receptor sensory rhodopsin II (pSRII) from Natronomonas pharaonis, as a proof of principle. The overall quality of the structure ensemble is good (backbone r.m.s. deviation of 0.48 Å) and agrees well with previously determined X-ray structures. Furthermore, measurements in more native-like small phospholipid bicelles indicate that the protein structure is the same as in detergent micelles, suggesting that environment-specific effects are minimal when using mild detergents. We use our case study as a platform to discuss the feasibility of similar solution NMR studies for other 7TM proteins, including members of the family of G protein–coupled receptors.
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Cites
Protein backbone angle restraints from searching a database for chemical shift and sequence homology
Cornilescu, G., Delaglio, F., & Bax, A. (1999) · Journal of Biomolecular NMR · doi:10.1023/a:1008392405740 · Cited by 2,957 · cited 1×
“Backbone torsion angle restraints were determined from chemical-shift data using TALO”
MethodCrystal structure of opsin in its G-protein-interacting conformation
Scheerer, P., Park, J. H., Hildebrand, P. W., Kim, Y. J., Krauß, N., Choe, H.-W., Hofmann, K. P., & Ernst, O. P. (2008) · Nature · doi:10.1038/nature07330 · Cited by 1,059
Cited by
Hyberts, S. G., Milbradt, A. G., Wagner, A. B., Arthanari, H., & Wagner, G. (2012) · Journal of Biomolecular NMR · doi:10.1007/s10858-012-9611-z · Cited by 450
The Magic of Bicelles Lights Up Membrane Protein Structure
Dürr, U. H. N., Gildenberg, M., & Ramamoorthy, A. (2012) · Chemical Reviews · doi:10.1021/cr300061w · Cited by 362
Mitra, S., Traughber, C. A., Brannon, M. K., Gomez, S., & Capelluto, D. G. S. (2013) · Journal of Biological Chemistry · doi:10.1074/jbc.m113.484170 · Cited by 46
Metadata
- Authors
- Gautier, A., Mott, H. R., Bostock, M. J., Kirkpatrick, J. P., & Nietlispach, D.
- Venue
- Nature Structural & Molecular Biology
- Published
- 2010-05-30
- Volume
- 17
- Issue
- 6
- Pages
- 768-774
- ISSN
- 1545-9985 (ISSN-L)
- 1545-9993
- OpenAlex
- W1971026764
- Cited by
- 212
- References
- 2
- FWCI
- 11.58
- Type
- journal article
- Language
- en
- Open access
- green
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- Gautier, A., Mott, H. R., Bostock, M. J., Kirkpatrick, J. P., & Nietlispach, D. (2010). Structure determination of the seven-helix transmembrane receptor sensory rhodopsin II by solution NMR spectroscopy. Nature Structural & Molecular Biology. https://doi.org/10.1038/nsmb.1807
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