Key interactions in HIV-1 maturation identified by hydrogen-deuterium exchange
Abstract
To characterize the intersubunit interactions underlying assembly and maturation in HIV-1, we determined the amide hydrogen exchange protection pattern of capsid protein in the immature virion and the mature virion using mass spectrometry. Alterations in protection upon maturation provide evidence for the maturation-induced formation of an interaction between the N- and C-terminal domains in half of the capsid molecules, indicating that only half of the capsid protein is assembled into the conical core.
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Only approximately two thirds of the CA molecules within the immature lattice are used to assemble the mature core.
“only approximately two thirds of the CA molecules within immature lattice are used to assemble the mature core [47,83,84].”
From Retrovirus maturation-an extraordinary structural transformation (2016) · cites this paper as [84]
Cites
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Absence of S6K1 protects against age- and diet-induced obesity while enhancing insulin sensitivity
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Structure of Full-Length HIV-1 CA: A Model for the Mature Capsid Lattice
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Mortuza, G. B., Haire, L. F., Stevens, A., Smerdon, S. J., Stoye, J. P., & Taylor, I. A. (2004) · Nature · doi:10.1038/nature02915 · Cited by 185 · see citing works
Retroviral proteases and their roles in virion maturation
Konvalinka, J., Kräusslich, H.-G., & Müller, B. (2015) · Virology · doi:10.1016/j.virol.2015.03.021 · Cited by 148 · see citing works
Bharat, T. A. M., Castillo Menendez, L. R., Hagen, W. J. H., Lux, V., Igonet, S., Schorb, M., Schur, F. K. M., Kräusslich, H.-G., & Briggs, J. A. G. (2014) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1401455111 · Cited by 107 · see citing works
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Retrovirus maturation-an extraordinary structural transformation
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“Firstly, only approximately two thirds of the CA molecules within immature lattice are used to assemble the mature core [47,83,84].”
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Hydrogen/Deuterium Exchange Analysis of HIV-1 Capsid Assembly and Maturation
Monroe, E. B., Kang, S., Kyere, S. K., Li, R., & Prevelige, P. E. (2010) · Structure · doi:10.1016/j.str.2010.08.016 · Cited by 49 · see citing works
Self-citationBonnard, D., Le Rouzic, E., Eiler, S., Amadori, C., Orlov, I., Bruneau, J.-M., Brias, J., Barbion, J., Chevreuil, F., Spehner, D., et al. (2018) · Journal of Biological Chemistry · doi:10.1074/jbc.m117.816793 · Cited by 42 · see citing works
- Authors
- Lanman, J., Lam, T. T., Emmett, M. R., Marshall, A. G., Sakalian, M., & Prevelige, P. E.
- Affiliations
- University of Alabama at Birmingham (US)
- Florida State University (US)
- University of Oklahoma Health Sciences Center (US)
- Corresponding author
- Prevelige, Peter E
- Venue
- Nature Structural & Molecular Biology
- Published
- 2004-06-20
- Volume
- 11
- Issue
- 7
- Pages
- 676-677
- ISSN
- 1545-9985 (ISSN-L)
- 1545-9993
- OpenAlex
- W1991485090
- Cited by
- 164
- References
- 3
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 1 · 2023: 1 · 2022: 3 · 2021: 5 · 2020: 5 · 2019: 5 · 2018: 3 · 2017: 5 · 2016: 5 · 2015: 10 · 2014: 12 · 2013: 11 · 2012: 5
- FWCI
- 3.94
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Structural & Molecular Biology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National High Magnetic Field Laboratory
- National Institute of Allergy and Infectious Diseases (AI 44626, R01AI044626)
- Topics
- Life Sciences › Immunology and Microbiology › Virology › HIV Research and Treatment
- Physical Sciences › Chemistry › Spectroscopy › Mass Spectrometry Techniques and Applications
- Health Sciences › Medicine › Infectious Diseases › HIV/AIDS drug development and treatment
- Keywords
- HIV-1, virus maturation, capsid protein, intersubunit interactions, hydrogen-deuterium exchange, immature virions, mature virion, capsid assembly, N-terminal domain, C-terminal domain
- MeSH terms
- Deuterium, Hydrogen, HIV-1, Spectrometry, Mass, Electrospray Ionization
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