Electrophysiological Characterization of Left Ventricular Myocytes from Obese Sprague‐Dawley Rat
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Objective: Obesity is a complex multifactorial disease that is often associated with cardiac arrhythmias. Various animal models have been used extensively to study the effects of obesity on physiological functions, but, to our knowledge, no study related to ionic membrane currents has been performed on isolated cardiac myocytes. Therefore, we examined the electrophysiological characteristics of four ionic currents from isolated left ventricular myocytes of a high‐energy (HE)‐induced obesity rat model. Research Methods and Procedures: Male Sprague‐Dawley rats were fed with either a control diet or a diet containing 33% kcal as fat (HE) for 14 weeks starting at 6 weeks of age. Voltage‐clamp experiments were performed on ventricular myocytes. Leptin receptor (ObR) expression was measured using ObR enzyme‐linked immunosorbent assay. Results: In the HE group, rats designated as obese did not develop a cardiac hypertrophy, either at the organ level or at the cellular level. Densities and kinetics of the L‐type calcium current, the transient outward potassium current, the delayed rectifier potassium current, and the sodium‐calcium exchange current (I NCX) were not significantly different between control and obese rats. A down‐regulation of ObR expression was evidenced in the heart of obese rats compared with controls. Acute exposure (5 minutes) of leptin (100 nM) did not induce a significant modification in the current densities either in control or in obese rats, except for I NCX density measured in control rats. Discussion: The absence of effect of leptin on I NCX in obese rats could be a potential arrhythmogenic substrate in obesity.
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High-fat feeding of Sprague-Dawley rats for up to 14 wk has not been associated with LVH despite the development of obesity.
“Likewise, high-fat feeding of Sprague-Dawley rats for up to 14 wk has not been associated with LVH despite the development of obesity (40, 299), whereas 7 wk of high-fat feeding in Wistar rats results in LVH (269).”
From Cardiac remodeling in obesity (2008) · cites this paper as [299]
A similar lack of LVH was observed following a 14-wk high-fat diet in Sprague-Dawley rats.
“A similar lack of LVH was observed independently by another group following a 14-wk high-fat diet in Sprague-Dawley rats (299).”
From Cardiac remodeling in obesity (2008) · cites this paper as [299]
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References 24 · Cited by 33
Research works printed but not linked: 1
- 1930739x, 2006, 5, Downloaded from https://onlinelibrary.wiley.com/doi/10.1038/oby.2006.90 by Test, Wiley Online Library on [20/08/2023]. See the Terms and Conditions (https://onlinelibrary.wiley.com/terms-and-conditions) on Wiley Online Library for rules of use; OA articles are governed by the applicable Creative Commons License · DOI
- Authors
- Ricci, E., Smallwood, S., Chouabe, C., Mertani, H. C., Raccurt, M., Morel, G., & Bonvallet, R.
- Affiliations
- Lyon 1 Université (FR)
- Corresponding author
- Bonvallet, Robert
- Venue
- Obesity
- Publisher
- Wiley
- Published
- 2006-05
- Volume
- 14
- Issue
- 5
- Pages
- 778-786
- ISSN
- 1930-7381 (ISSN-L)
- 1930-739X
- OpenAlex
- W2052143010
- Cited by
- 33
- References
- 24
- Citations by year
- 2026: 1 · 2023: 1 · 2022: 1 · 2021: 4 · 2020: 2 · 2018: 2 · 2017: 1 · 2016: 1 · 2015: 2 · 2014: 2 · 2013: 2 · 2012: 1
- FWCI
- 1.41
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Obesity (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Funders
- European Commission
- Topics
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiac electrophysiology and arrhythmias
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiovascular Function and Risk Factors
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiac Arrhythmias and Treatments
- Keywords
- left ventricular myocytes, ionic currents, L-type calcium current, transient outward potassium current, delayed rectifier potassium current, leptin receptor, leptin, cardiac arrhythmias, high-fat diet, cardiac hypertrophy, arrhythmogenic substrate
- MeSH terms
- Animals, Body Weight, Electrophysiology, Heart Ventricles, Male, Membrane Potentials, Obesity, Organ Size, Receptors, Cell Surface, Rats, Sprague-Dawley, Leptin, Myocytes, Cardiac, Cell Size, Adiposity, Rats, Adipose Tissue, White, Receptors, Leptin
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