In utero nicotine exposure promotes M2 activation in neonatal mouse alveolar macrophages
Open accessAbstract
Background Maternal smoking in utero has been associated with adverse health outcomes including lower respiratory tract infections in infants and children, but the mechanisms underlying these associations continue to be investigated. We hypothesized that nicotine plays a significant role in mediating the effects of maternal tobacco smoke on the function of the neonatal alveolar macrophage (AM), the resident immune cell in the neonatal lung.Methods Primary AMs were isolated at postnatal day 7 from a murine model of in utero nicotine exposure. The murine AM cell line MH-S was used for additional in vitro studies.Results In utero nicotine increased interleukin-13 and transforming growth factor-β1 (TGFβ1) in the neonatal lung. Nicotine-exposed AMs demonstrated increased TGFβ1 and increased markers of alternative activation with diminished phagocytic function. However, AMs from mice deficient in the α7 nicotinic acetylcholine receptor (α7 nAChR) had less TGFβ1, reduced alternative activation, and improved phagocytic functioning despite similar in utero nicotine exposure.Conclusion In utero nicotine exposure, mediated in part via the α7 nAChR, may increase the risk of lower respiratory tract infections in neonates by changing the resting state of AM toward alternative activation. These findings have important implications for immune responses in the nicotine-exposed neonatal lung.
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Neonatal mice exposed to nicotine in utero had enhanced M2 phenotype in their alveolar macrophages.
“Neonatal mice exposed to nicotine in utero had enhanced M2 phenotype in their alveolar macrophages.65”
From Impact of Tobacco Smoke and Nicotine Exposure on Lung Development (2016) · cites this paper as [65]
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References 39 · Cited by 31
Research works printed but not linked: 1
- [16]Tong VT Jones JR Dietz PM D’Angelo D Bombard JM Trends in smoking before, during, and after pregnancy - Pregnancy Risk Assessment Monitoring System (PRAMS), United States, 31 sites, 2000–2005 MMWR Surveill Summ 2009 58 1 29 19478726
- Authors
- Wongtrakool, C., Grooms, K., Ping, X.-D., Rivera, H., Ward, J., Roser-Page, S., Roman, J., Brown, L. A. S., & Gauthier, T. W.
- Affiliations
- Emory University (US)
- University of Louisville (US)
- Corresponding author
- Wongtrakool, Cherry
- Venue
- Pediatric Research
- Published
- 2012-05-04
- Volume
- 72
- Issue
- 2
- Pages
- 147-153
- ISSN
- 0031-3998 (ISSN-L)
- 1530-0447
- PMC
- PMC3600420
- OpenAlex
- W2024190010
- Cited by
- 31
- References
- 39
- Citations by year
- 2026: 2 · 2024: 1 · 2023: 1 · 2022: 2 · 2021: 4 · 2020: 2 · 2019: 1 · 2017: 4 · 2016: 2 · 2015: 4 · 2014: 4 · 2013: 3 · 2012: 1
- FWCI
- 2.57
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Pediatric Research (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health
- National Heart, Lung, and Blood Institute (K08HL080293)
- Topics
- Health Sciences › Medicine › Physiology › Asthma and respiratory diseases
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Neonatal Respiratory Health Research
- Health Sciences › Medicine › Pediatrics, Perinatology and Child Health › Prenatal Substance Exposure Effects
- Keywords
- maternal smoking, alveolar macrophages, M2 macrophage polarization, alternative macrophage activation, TGF-β1, IL-13, alpha7 nicotinic acetylcholine receptor, phagocytosis, neonatal lung, lower respiratory tract infection
- MeSH terms
- Animals, Bungarotoxins, Cell Line, Female, Fibronectins, Fluorescent Antibody Technique, Immunohistochemistry, Macrophage Activation, Macrophages, Mice, Inbred C57BL, Nicotine, Pregnancy, Prenatal Exposure Delayed Effects, Pulmonary Alveoli, Receptors, Nicotinic, Smoking, Blotting, Western, Mice, Knockout, Paracrine Communication, Mice, Real-Time Polymerase Chain Reaction, alpha7 Nicotinic Acetylcholine Receptor
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