The DOI matches its Crossref record; 30 of the 30 references it cites were found in a registry or an index.
4-Coumarate 3-hydroxylase in the lignin biosynthesis pathway is a cytosolic ascorbate peroxidase
Open accessAbstract
Lignin biosynthesis is evolutionarily conserved among higher plants and features a critical 3-hydroxylation reaction involving phenolic esters. However, increasing evidence questions the involvement of a single pathway to lignin formation in vascular plants. Here we describe an enzyme catalyzing the direct 3-hydroxylation of 4-coumarate to caffeate in lignin biosynthesis as a bifunctional peroxidase that oxidizes both ascorbate and 4-coumarate at comparable rates. A combination of biochemical and genetic evidence in the model plants Brachypodium distachyon and Arabidopsis thaliana supports a role for this coumarate 3-hydroxylase (C3H) in the early steps of lignin biosynthesis. The subsequent efficient O-methylation of caffeate to ferulate in grasses is substantiated by in vivo biochemical assays. Our results identify C3H as the only non-membrane bound hydroxylase in the lignin pathway and revise the currently accepted models of lignin biosynthesis, suggesting new gene targets to improve forage and bioenergy crops.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Reactive oxygen gene network of plants
Mittler, R., Vanderauwera, S., Gollery, M., & Van Breusegem, F. (2004) · Trends in Plant Science · doi:10.1016/j.tplants.2004.08.009 · Cited by 5,579
Self-citationStress-Induced Phenylpropanoid Metabolism.
Dixon, R. A., & Paiva, N. L. (1995) · The Plant Cell · doi:10.1105/tpc.7.7.1085 · Cited by 4,634
Self-citationLignin Valorization: Improving Lignin Processing in the Biorefinery
Ragauskas, A. J., Beckham, G. T., Biddy, M. J., Chandra, R., Chen, F., Davis, M. F., Davison, B. H., Dixon, R. A., Gilna, P., Keller, M., et al. (2014) · Science · doi:10.1126/science.1246843 · Cited by 4,125
Self-citationDavletova, S., Rizhsky, L., Liang, H., Shengqiang, Z., Oliver, D. J., Coutu, J., Shulaev, V., Schlauch, K., & Mittler, R. (2005) · The Plant Cell · doi:10.1105/tpc.104.026971 · Cited by 990
Self-citationThe origin and evolution of lignin biosynthesis
Weng, J., & Chapple, C. (2010) · New Phytologist · doi:10.1111/j.1469-8137.2010.03327.x · Cited by 824
Caffeoyl Shikimate Esterase (CSE) Is an Enzyme in the Lignin Biosynthetic Pathway in Arabidopsis
Vanholme, R., Cesarino, I., Rataj, K., Xiao, Y., Sundin, L., Goeminne, G., Kim, H., Cross, J., Morreel, K., Araujo, P., et al. (2013) · Science · doi:10.1126/science.1241602 · Cited by 521
Schoch, G., Goepfert, S., Morant, M., Hehn, A., Meyer, D., Ullmann, P., & Werck-Reichhart, D. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m104047200 · Cited by 465
Koussevitzky, S., Suzuki, N., Huntington, S., Armijo, L., Sha, W., Cortes, D., Shulaev, V., & Mittler, R. (2008) · Journal of Biological Chemistry · doi:10.1074/jbc.m806337200 · Cited by 412
Self-citationKawasaki, T., Koita, H., Nakatsubo, T., Hasegawa, K., Wakabayashi, K., Takahashi, H., Umemura, K., Umezawa, T., & Shimamoto, K. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0509875103 · Cited by 372
Pnueli, L., Liang, H., Rozenberg, M., & Mittler, R. (2003) · The Plant Journal · doi:10.1046/j.1365-313x.2003.01715.x · Cited by 334
Self-citation
Cited by
Contribution of phenylpropanoid metabolism to plant development and plant–environment interactions
Dong, N., & Lin, H. (2021) · Journal of Integrative Plant Biology · doi:10.1111/jipb.13054 · Cited by 1,748
Plant Phenylalanine/Tyrosine Ammonia-lyases
Barros, J., & Dixon, R. A. (2020) · Trends in Plant Science · doi:10.1016/j.tplants.2019.09.011 · Cited by 348
Self-citationMetabolic flux analysis of secondary metabolism in plants
Shih, M.-L., & Morgan, J. A. (2020) · Metabolic Engineering Communications · doi:10.1016/j.mec.2020.e00123 · Cited by 85
Metadata
- Authors
- Barros, J., Escamilla-Trevino, L., Song, L., Rao, X., Serrani-Yarce, J. C., Palacios, M. D., Engle, N., Choudhury, F. K., Tschaplinski, T. J., Venables, B. J., Mittler, R., & Dixon, R. A.
- Venue
- Nature Communications
- Published
- 2019-04-30
- Volume
- 10
- Issue
- 1
- Pages
- 1994-1994
- ISSN
- 2041-1723 (ISSN-L)
- OpenAlex
- W2942587370
- Cited by
- 283
- References
- 30
- FWCI
- 8.88
- Type
- journal article
- Language
- en
- Open access
- gold
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: 4-Coumarate 3-hydroxylase in the lignin biosynthesis pathway is a cytosolic ascorbate peroxidase. https://app.cochat.ai/papers/10.1038/s41467-019-10082-7
- The paper
- Barros, J., Escamilla-Trevino, L., Song, L., Rao, X., Serrani-Yarce, J. C., Palacios, M. D., Engle, N., Choudhury, F. K., Tschaplinski, T. J., Venables, B. J., Mittler, R., & Dixon, R. A. (2019). 4-Coumarate 3-hydroxylase in the lignin biosynthesis pathway is a cytosolic ascorbate peroxidase. Nature Communications. https://doi.org/10.1038/s41467-019-10082-7
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
