RSL24D1 sustains steady-state ribosome biogenesis and pluripotency translational programs in embryonic stem cells
Open accessPublished version of a preprint
Abstract
Embryonic stem cell (ESC) fate decisions are regulated by a complex circuitry that coordinates gene expression at multiple levels from chromatin to mRNA processing. Recently, ribosome biogenesis and translation have emerged as key pathways that efficiently control stem cell homeostasis, yet the underlying molecular mechanisms remain largely unknown. Here, we identified RSL24D1 as highly expressed in both mouse and human pluripotent stem cells. RSL24D1 is associated with nuclear pre-ribosomes and is required for the biogenesis of 60S subunits in mouse ESCs. Interestingly, RSL24D1 depletion significantly impairs global translation, particularly of key pluripotency factors and of components from the Polycomb Repressive Complex 2 (PRC2). While having a moderate impact on differentiation, RSL24D1 depletion significantly alters ESC self-renewal and lineage commitment choices. Altogether, these results demonstrate that RSL24D1-dependant ribosome biogenesis is both required to sustain the expression of pluripotent transcriptional programs and to silence PRC2-regulated developmental programs, which concertedly dictate ESC homeostasis.
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Cites
Global quantification of mammalian gene expression control
Schwanhäusser, B., Busse, D., Li, N., Dittmar, G., Schuchhardt, J., Wolf, J., Chen, W., & Selbach, M. (2011) · Nature · doi:10.1038/nature10098 · Cited by 6,566 · cited 1× · see citing works
“3 circumstances, messenger RNA (mRNA) and protein levels are poorly correlated, 62 including in ESCs and iPSCs, therefore highlighting the importance of translational 63 regulations for shaping the cellular proteome landscape required for cell fate changes 64 (10-15).”
BackgroundChIP‐Atlas: a data‐mining suite powered by full integration of public ChIP‐seq data
Oki, S., Ohta, T., Shioi, G., Hatanaka, H., Ogasawara, O., Okuda, Y., Kawaji, H., Nakaki, R., Sese, J., & Meno, C. (2018) · The EMBO Reports · doi:10.15252/embr.201846255 · Cited by 763 · see citing works
Single Cell RNA-Sequencing of Pluripotent States Unlocks Modular Transcriptional Variation
Kolodziejczyk, A. A., Kim, J. K., Tsang, J. C. H., Ilicic, T., Henriksson, J., Natarajan, K. N., Tuck, A. C., Gao, X., Bühler, M., Liu, P., et al. (2015) · Cell Stem Cell · doi:10.1016/j.stem.2015.09.011 · Cited by 570 · see citing works
Cruz-Molina, S., Respuela, P., Tebartz, C., Kolovos, P., Nikolic, M., Fueyo, R., van Ijcken, W. F. J., Grosveld, F., Frommolt, P., Bazzi, H., et al. (2017) · Cell Stem Cell · doi:10.1016/j.stem.2017.02.004 · Cited by 240 · see citing works
Bulut-Karslioglu, A., Macrae, T. A., Oses-Prieto, J. A., Covarrubias, S., Percharde, M., Ku, G., Diaz, A., McManus, M. T., Burlingame, A. L., & Ramalho-Santos, M. (2018) · Cell Stem Cell · doi:10.1016/j.stem.2018.02.004 · Cited by 97 · see citing works
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- Authors
- Durand, S., Bruelle, M., Bourdelais, F., Bennychen, B., Blin-Gonthier, J., Isaac, C., Huyghe, A., Martel, S., Seyve, A., Vanbelle, C., Adrait, A., Couté, Y., Meyronet, D., Catez, F., Diaz, J.-J., Lavial, F., Ricci, E. P., Ducray, F., & Gabut, M.
- Affiliations
- Lyon 1 Université (FR)
- University of Ottawa (CA)
- Centre National de la Recherche Scientifique (FR)
- École Normale Supérieure de Lyon (FR)
- Corresponding author
- Gabut, Mathieu
- ORCID
- Venue
- Nature Communications
- Published
- 2023-01-23
- Volume
- 14
- Issue
- 1
- Pages
- 356-356
- ISSN
- 2041-1723 (ISSN-L)
- PMC
- PMC9870888
- OpenAlex
- W4317718280
- Cited by
- 21
- References
- 5
- Citations by year
- 2026: 4 · 2025: 5 · 2024: 7 · 2023: 3 · 2022: 2
- FWCI
- 1.69
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Nature Communications (publishedVersion, cc-by)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion)
- Research Square (acceptedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- HAL (submittedVersion, cc-by)
- NPARC (submittedVersion, cc-by)
- RePEc: Research Papers in Economics (submittedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- European Commission (631794, ANR-17-EURE-0003)
- Agence Nationale de la Recherche (ANR-10-INBS-08, ANR-17-EURE-0003, ANR-17-EURE-, Proteomics French Infrastructure,ANR-10-INBS-08, ANR-17, ANR-10, ANR-17-CONV-0002)
- Institut National de la Santé et de la Recherche Médicale
- University of Toronto
- Ligue Contre le Cancer
- Institut National Du Cancer
- Samsung Biomedical Research Institute
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Keywords
- ribosome biogenesis, embryonic stem cells, pluripotency, translation (biology), 60S ribosomal subunit, polycomb repressive complex 2, self-renewal, lineage commitment, stem cell fate, pluripotency factors
- MeSH terms
- Animals, Cell Differentiation, Humans, Pluripotent Stem Cells, Mice, Embryonic Stem Cells, Polycomb Repressive Complex 2
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