Microbiota alters the metabolome in an age- and sex- dependent manner in mice
Open accessAbstract
Commensal bacteria are major contributors to mammalian metabolism. We used liquid chromatography mass spectrometry to study the metabolomes of germ-free, gnotobiotic, and specific-pathogen-free mice, while also evaluating the influence of age and sex on metabolite profiles. Microbiota modified the metabolome of all body sites and accounted for the highest proportion of variation within the gastrointestinal tract. Microbiota and age explained similar amounts of variation the metabolome of urine, serum, and peritoneal fluid, while age was the primary driver of variation in the liver and spleen. Although sex explained the least amount of variation at all sites, it had a significant impact on all sites except the ileum. Collectively, these data illustrate the interplay between microbiota, age, and sex in the metabolic phenotypes of diverse body sites. This provides a framework for interpreting complex metabolic phenotypes and will help guide future studies into the role that the microbiome plays in disease.
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Secondary bile acid concentrations are significantly higher in the intestines and fecal samples of SPF mice compared with GF mice, providing strong evidence for their microbial origin.
“This phenomenon is evidenced by the significantly higher concentration of secondary BAs in the intestines and fecal samples of SPF mice compared to GF mice, providing strong evidence for their microbial origin.351–353”
From Microbiota–gut–brain axis and its therapeutic applications in neurodegenerative diseases (2024) · cites this paper as [352]
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References 38 · Cited by 49
Research works printed but not linked: 1
- [3]M. Kleiber, The fire of life: An introduction to animal energetics. (John Wiley & Sons, Inc., New York: London, 1961).
- Authors
- Brown, K., Thomson, C. A., Wacker, S., Drikic, M., Groves, R., Fan, V., Lewis, I. A., & McCoy, K. D.
- Affiliations
- University of Calgary (CA)
- Corresponding author
- McCoy, Kathy D.
- ORCID
- Venue
- Nature Communications
- Published
- 2023-03-11
- Volume
- 14
- Issue
- 1
- Pages
- 1348-1348
- ISSN
- 2041-1723 (ISSN-L)
- PMC
- PMC10008592
- OpenAlex
- W4323928719
- Cited by
- 49
- References
- 38
- Citations by year
- 2026: 14 · 2025: 13 · 2024: 15 · 2023: 7
- FWCI
- 4.35
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Nature Communications (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- RePEc: Research Papers in Economics (submittedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Canadian Network for Research and Innovation in Machining Technology, Natural Sciences and Engineering Research Council of Canada (Scholarship)
- Alberta Innovates | Alberta Innovates - Health Solutions (Scholarship)
- Gouvernement du Canada | Natural Sciences and Engineering Research Council of Canada (DG04547)
- Gouvernement du Canada | Canadian Institutes of Health Research (PJT-165930)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
- Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
- Keywords
- gut microbiota, metabolome, sex differences, germ-free mice, gnotobiotic mice, commensal bacteria, gastrointestinal tract, urine, serum, peritoneal fluid, liver, spleen, ileum
- MeSH terms
- Animals, Germ-Free Life, Mammals, Specific Pathogen-Free Organisms, Gastrointestinal Tract, Mice, Metabolomics, Metabolome, Microbiota
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