An integrated proteome and transcriptome of B cell maturation defines poised activation states of transitional and mature B cells
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Abstract
During B cell maturation, transitional and mature B cells acquire cell-intrinsic features that determine their ability to exit quiescence and mount effective immune responses. Here we use label-free proteomics to quantify the proteome of B cell subsets from the mouse spleen and map the differential expression of environmental sensing, transcription, and translation initiation factors that define cellular identity and function. Cross-examination of the full-length transcriptome and proteome identifies mRNAs related to B cell activation and antibody secretion that are not accompanied by detection of the encoded proteins. In addition, proteomic data further suggests that the translational repressor PDCD4 restrains B cell responses, in particular those from marginal zone B cells, to a T-cell independent antigen. In summary, our molecular characterization of B cell maturation presents a valuable resource to further explore the mechanisms underpinning the specialized functions of B cell subsets, and suggest the presence of ‘poised’ mRNAs that enable expedited B cell responses.
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- Authors
- Salerno, F., Howden, A. J. M., Matheson, L. S., Gizlenci, Ö., Screen, M., Lingel, H., Brunner-Weinzierl, M. C., & Turner, M.
- Affiliations
- Babraham Institute (GB)
- University of Dundee (GB)
- Otto-von-Guericke-Universität Magdeburg (DE)
- Corresponding author
- Salerno, Fiamma
- Turner, Martin
- ORCID
- Venue
- Nature Communications
- Published
- 2023-08-23
- Volume
- 14
- Issue
- 1
- Pages
- 5116-5116
- ISSN
- 2041-1723 (ISSN-L)
- PMC
- PMC10447577
- OpenAlex
- W4386095572
- Cited by
- 29
- References
- 12
- Citations by year
- 2026: 11 · 2025: 9 · 2024: 7 · 2023: 2
- FWCI
- 3.18
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Nature Communications (publishedVersion, cc-by)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Discovery Research Portal (University of Dundee) (submittedVersion, other-oa)
- RePEc: Research Papers in Economics (submittedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- University of Dundee
- Wellcome Trust (200823/Z/16/Z)
- Cancer Research UK
- European Commission (841930)
- Deutsche Forschungsgemeinschaft (Br1860/12, SFB-854, SFB854 B14)
- Directorate for Biological Sciences
- Horizon 2020 Framework Programme (765158)
- Biotechnology and Biological Sciences Research Council (BBS/E/B/000C0428, BBS/E/B/000C0409, BBS/E/B/000C0407, BBS/E/B/000C0427)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Keywords
- B cell maturation, transitional B cells, marginal zone B cells, proteome, transcriptome, PDCD4, protein synthesis inhibition, B cell activation, immunoglobulin secretion, T-independent antigens, environmental sensing, translation initiation factors, spleen
- MeSH terms
- Animals, B-Lymphocytes, Cell Differentiation, RNA, Messenger, Transcription Factors, Protein Biosynthesis, B-Lymphocyte Subsets, RNA-Binding Proteins, Proteome, Apoptosis Regulatory Proteins, Mice, Transcriptome
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