Quantitative determination of the spatial distribution of components in single cells with CellDetail
Open accessPublished version of a preprint
Abstract
The distribution of biomolecules within cells changes upon aging and diseases. To quantitatively determine the spatial distribution of components inside cells, we built the user-friendly open-source 3D-cell-image analysis platform Cell Det ection and A nalysis of I ntensity L ounge (CellDetail). The algorithm within CellDetail is based on the concept of the dipole moment. CellDetail provides quantitative values for the distribution of the polarity proteins Cdc42 and Tubulin in young and aged hematopoietic stem cells (HSCs). Septin proteins form networks within cells that are critical for cell compartmentalization. We uncover a reduced level of organization of the Septin network within aged HSCs and within senescent human fibroblasts. Changes in the Septin network structure might therefore be a common feature of aging. The level of organization of the network of Septin proteins in aged HSCs can be restored to a youthful level by pharmacological attenuation of the activity of the small RhoGTPase Cdc42.
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Cites
Parrinello, S., Coppe, J.-P., Krtolica, A., & Campisi, J. (2005) · Journal of Cell Science · doi:10.1242/jcs.01635 · Cited by 631 · see citing works
Hippocampal Neuronal Polarity Specified by Spatially Localized mPar3/mPar6 and PI 3-Kinase Activity
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Cdc42 activity regulates hematopoietic stem cell aging and rejuvenation
Florian, M. C., Dörr, K., Niebel, A., Daria, D., Schrezenmeier, H., Rojewski, M., Filippi, M.-D., Hasenberg, A., Gunzer, M., Scharffetter-Kochanek, K., et al. (2012) · Cell Stem Cell · doi:10.1016/j.stem.2012.04.007 · Cited by 564 · see citing works
Self-citationIntegrated intracellular organization and its variations in human iPS cells
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- Authors
- Schuster, T., Amoah, A., Vollmer, A., Marka, G., Niemann, J., Saçma, M., Sakk, V., Soller, K., Vogel, M., Grigoryan, A., Wlaschek, M., Scharffetter-Kochanek, K., Mulaw, M., & Geiger, H.
- Affiliations
- Universität Ulm (DE)
- Corresponding author
- Schuster, Tanja
- Geiger, Hartmut
- ORCID
- Venue
- Nature Communications
- Published
- 2024-11-26
- Volume
- 15
- Issue
- 1
- Pages
- 10250-10250
- ISSN
- 2041-1723 (ISSN-L)
- PMC
- PMC11599593
- OpenAlex
- W4404747465
- Cited by
- 7
- References
- 5
- Citations by year
- 2026: 3 · 2025: 4
- FWCI
- 1.34
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Nature Communications (publishedVersion, cc-by)
- Research Square (acceptedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- RePEc: Research Papers in Economics (submittedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- OPen Access Repositorium der Universität Ulm (OPARU) (Ulm University) (submittedVersion, cc-by)
- Universität Ulm, Kommunikations- und Informationszentrum (kiz) (cc-by)
- Funders
- Deutsche Forschungsgemeinschaft (FOR 2674, SFB 1506)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Cell Image Analysis Techniques
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
- Keywords
- dipole moment, Cdc42, tubulin, hematopoietic stem cells, cellular aging, senescence, fibroblasts, Rho GTPases, intracellular protein distribution, open source software
- MeSH terms
- Algorithms, Animals, Fibroblasts, Hematopoietic Stem Cells, Humans, Tubulin, Cellular Senescence, cdc42 GTP-Binding Protein, Imaging, Three-Dimensional, Mice, Septins, Single-Cell Analysis
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