The gene TaWOX5 overcomes genotype dependency in wheat genetic transformation
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Abstract
Although great progress has been achieved regarding wheat genetic transformation technology in the past decade 1-3, genotype dependency, the most impactful factor in wheat genetic transformation, currently limits the capacity for wheat improvement by transgenic integration and genome-editing approaches. The application of regeneration-related genes during in vitro culture could potentially contribute to enhancement of plant transformation efficiency 4-11. In the present study, we found that overexpression of the wheat gene TaWOX5 from the WUSCHEL family dramatically increases transformation efficiency with less genotype dependency than other methods. The expression of TaWOX5 in wheat calli prohibited neither shoot differentiation nor root development. Moreover, successfully transformed transgenic wheat plants can clearly be recognized based on a visible botanic phenotype, relatively wider flag leaves. Application of TaWOX5 improved wheat immature embryo transformation and regeneration. The use of TaWOX5 in improvement of transformation efficiency also showed promising results in Triticum monococcum, triticale, rye, barley and maize.
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Overexpression of the T. aestivum WUSCHEL-related homeobox 5 (TaWOX5) gene overcomes genotype dependency in wheat, barley, and maize genetic transformation.
“The latest breakthrough is the overexpression of the T. aestivum WUSCHEL-related homeobox 5 (TaWOX5) gene to overcome genotype dependency in wheat, barley, and maize genetic transformation (Wang et al., 2022).”
From Genetic resources and precise gene editing for targeted improvement of barley abiotic stress tolerance (2023) · cites this paper as Wang et al., 2022
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“The latest breakthrough is the overexpression of the T. aestivum WUSCHEL-related homeobox 5 ( TaWOX5 ) gene to overcome genotype dependency in wheat, barley, and maize genetic transformation (Wang et al., 2022).”
- Authors
- Wang, K., Shi, L., Liang, X., Zhao, P., Wang, W., Liu, J., Chang, Y., Hiei, Y., Yanagihara, C., Du, L., Ishida, Y., & Ye, X.
- Affiliations
- Chinese Academy of Agricultural Sciences (CN)
- Capital Normal University (CN)
- Japan Tobacco (Japan) (JP)
- Corresponding author
- Wang, Ke
- Ishida, Yuji
- Ye, Xingguo
- Venue
- Nature Plants
- Published
- 2022-01-13
- Volume
- 8
- Issue
- 2
- Pages
- 110-117
- ISSN
- 2055-026X (ISSN-L)
- 2055-0278
- OpenAlex
- W4206244396
- Cited by
- 303
- References
- 26
- Citations by year
- 2026: 40 · 2025: 97 · 2024: 59 · 2023: 67 · 2022: 39
- FWCI
- 21.86
- Type
- journal article
- Language
- en
- Update notices
- correction (2022-05-30)
- Open access
- green
- doi.org
- Locations
- Nature Plants (publishedVersion)
- Research Square (acceptedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- Saint Louis University
- Science and Technology Department of Ningxia (2019BBF02020)
- National Natural Science Foundation of China (2060302-2-19, 31971946, 2060302)
- Chinese Academy of Agricultural Sciences (2060302-2-19, 2060302-2, 2060302)
- Institute of Crop Sciences, Chinese Academy of Agricultural Sciences
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Wheat and Barley Genetics and Pathology
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Genetically Modified Organisms Research
- Keywords
- transformation efficiency, immature embryo culture, callus regeneration, transgenic wheat, cereals, Triticum monococcum, triticale, rye, barley, maize
- MeSH terms
- Hordeum, Genotype, Transformation, Genetic, Triticum, Plants, Genetically Modified
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