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Cited by 55Polysaccharides and Plant Cell WallsPlant Surface Properties and TreatmentsGlycosylation and Glycoproteins Research

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Polymerization of the backbone of the pectic polysaccharide rhamnogalacturonan I

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Amos, Robert A., Atmodjo, Melani A., Huang, Chin, Gao, Zhongwei, Venkat, Aarya, Taujale, Rahil, Kannan, Natarajan, Moremen, Kelley W., Mohnen, Debra·2022·Nature Plants
DOI 10.1038/s41477-022-01270-3PMC PMC10115348

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Abstract

Rhamnogalacturonan I (RG-I) is a major plant cell wall pectic polysaccharide defined by its repeating disaccharide backbone structure of [4)-α-D-GalA-(1,2)-α-L-Rha-(1,]. A family of RG-I:Rhamnosyltransferases (RRT) has previously been identified, but synthesis of the RG-I backbone has not been demonstrated in vitro because the identity of Rhamnogalacturonan I:Galaturonosyltransferase (RG-I:GalAT) was unknown. Here a putative glycosyltransferase, At1g28240/MUCI70, is shown to be an RG-I:GalAT. The name RGGAT1 is proposed to reflect the catalytic activity of this enzyme. When incubated together with the rhamnosyltransferase RRT4, the combined activities of RGGAT1 and RRT4 result in elongation of RG-I acceptors in vitro into a polymeric product. RGGAT1 is a member of a new GT family categorized as GT116, which does not group into existing GT-A clades and is phylogenetically distinct from the GALACTURONOSYLTRANSFERASE (GAUT) family of GalA transferases that synthesize the backbone of the pectin homogalacturonan. RGGAT1 has a predicted GT-A fold structure but employs a metal-independent catalytic mechanism that is rare among glycosyltransferases with this fold type. The identification of RGGAT1 and the 8-member Arabidopsis GT116 family provides a new avenue for studying the mechanism of RG-I synthesis and the function of RG-I in plants.

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Metadata

Authors
Amos, R. A., Atmodjo, M. A., Huang, C., Gao, Z., Venkat, A., Taujale, R., Kannan, N., Moremen, K. W., & Mohnen, D.
Affiliations
University of Georgia (US)
Corresponding author
Mohnen, Debra
ORCID
Venue
Nature Plants
Publisher
Springer Science and Business Media LLC
Published
2022-11-10
Volume
8
Issue
11
Pages
1289-1303
ISSN
  • 2055-026X (ISSN-L)
  • 2055-0278
DOI
10.1038/s41477-022-01270-3
PMC
PMC10115348
OpenAlex
W4308695747
Cited by
55
References
1
Citations by year
2026: 19 · 2025: 14 · 2024: 14 · 2023: 8
FWCI
4.96
Type
journal article
Language
en
Update notices
correction (2023-03-20)
Open access
Licence
Locations
  • Nature Plants (publishedVersion)
  • Research Square (acceptedVersion, cc-by)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
  • OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information) (submittedVersion)
Funders
  • U.S. Department of Health & Human Services | National Institutes of Health (R35 GM139656, P41GM103390, R01GM130915)
  • U.S. Department of Energy (DE-SC0015662, AC05-000R22725)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Polysaccharides and Plant Cell Walls
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Surface Properties and Treatments
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Glycosylation and Glycoproteins Research
Keywords
rhamnogalacturonan I, pectin, rhamnosyltransferase, Arabidopsis thaliana, homogalacturonan
MeSH terms
Pectins, Polysaccharides, Glycosyltransferases, Arabidopsis, Polymerization

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