The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
ARF degradation defines a deeply conserved step in auxin response
Open accessPublished version of a preprint
Abstract
In land plants, the signalling molecule auxin profoundly controls growth and development, chiefly through a transcriptional response system. The auxin response is mediated by modulating the activity of DNA-binding auxin response factor (ARF) proteins. The concentrations and stoichiometry of the competing A- and B-class ARFs define cells’ capacity for auxin response. In the minimal auxin response system of the liverwort Marchantia polymorpha , both A- and B-ARFs are unstable, but the underlying mechanisms, developmental relevance and evolutionary history of this instability are unknown. Here we identify a minimal motif that is necessary for MpARF2 (B-class) degradation and show that it is critical for development and the auxin response. Through comparative analysis and motif swaps among all ARF classes in extant algae and land plants, we infer that the emergence of ARF instability probably occurred in the ancestor of the A- and B-ARF clades and, therefore, preceded or coincided with the origin of the auxin response system.
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Cites
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Mockaitis, K., & Estelle, M. (2008) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.23.090506.123214 · Cited by 651
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Metadata
- Authors
- de Roij, M., Hernández García, J., Das, S., Borst, J. W., & Weijers, D.
- Affiliations
- Wageningen University & Research (NL)
- Institute of Science and Technology Austria (AT)
- Corresponding author
- Weijers, Dolf
- Venue
- Nature Plants
- Published
- 2025-04-11
- Volume
- 11
- Issue
- 4
- Pages
- 717-724
- ISSN
- 2055-026X (ISSN-L)
- 2055-0278
- PMC
- PMC12014493
- OpenAlex
- W4409370926
- Cited by
- 16
- References
- 3
- Citations by year
- 2026: 10 · 2025: 6
- FWCI
- 8.65
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Locations
- Nature Plants (publishedVersion, cc-by)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Socio-Environmental Systems Modeling (submittedVersion, cc-by)
- Funders
- Human Frontier Science Program (RGP0015/2022)
- Nederlandse Organisatie voor Wetenschappelijk Onderzoek (OCENW.M20.031)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Light effects on plants
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Parasitism and Resistance
- Keywords
- auxin response, auxin response factor, protein degradation, Marchantia polymorpha, liverworts, land plant evolution
- MeSH terms
- Indoleacetic Acids, Plant Growth Regulators, Plant Proteins, Transcription Factors, Gene Expression Regulation, Plant, Marchantia
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