In situ architecture of the nuclear pore complex of the higher plant Arabidopsis thaliana
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Abstract
The nucleus is enclosed by the nuclear envelope, which contains nuclear pore complexes (NPCs). While NPCs have been well studied in vertebrates, yeast and algae, in situ structural data for higher plants is lacking. Here we show that individual nucleoporins of Arabidopsis thaliana and humans exhibit high structural similarity. We report an in situ NPC structure of higher plants, derived from A. thaliana root protoplasts using cryo-electron tomography, subtomogram averaging and homology-based integrative modelling. We present the AtNPC model based on predictions of A. thaliana nucleoporins (NUPs), supported by mass spectrometry. Here the AtNPC scaffold contains one Y-complex ring at the cytosolic and two at the nuclear ring. The AtNPC contains prominent NUP155 connector elements that are conserved in human NPCs but not in C hlamydomonas reinhardtii NPCs. Our model suggests that the ELYS homologue HOS1 plays an important role in the head-to-tail connection of Y-complexes in AtNPCs.
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References 101 · Cited by 10
- Authors
- Sanchez Carrillo, I. B., Hoffmann, P. C., Obarska-Kosinska, A., Fourcassié, V., Beck, M., & Germain, H.
- Affiliations
- Max Planck Institute of Biophysics (DE)
- Université Laval (CA)
- Goethe University Frankfurt (DE)
- Université du Québec à Trois-Rivières (CA)
- Corresponding author
- Beck, Martin
- Germain, Hugo
- ORCID
- Venue
- Nature Plants
- Published
- 2025-10-31
- Volume
- 11
- Issue
- 11
- Pages
- 2368-2380
- ISSN
- 2055-026X (ISSN-L)
- 2055-0278
- PMC
- PMC12626886
- OpenAlex
- W4415731717
- Cited by
- 10
- References
- 101
- Citations by year
- 2026: 10
- FWCI
- 2.99
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Locations
- Nature Plants (publishedVersion, cc-by)
- Research Square (acceptedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Le dépôt institutionnel (Université du Québec à Trois-Rivières) (submittedVersion, other-oa)
- MPG.PuRe (Max Planck Society) (submittedVersion, other-oa)
- Funders
- Government of Canada (RGPIN/4002-2020)
- European Commission
- Max-Planck-Gesellschaft
- Mitacs
- Natural Sciences and Engineering Research Council of Canada
- HORIZON EUROPE European Research Council (101054823)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Structural Biology › Advanced Electron Microscopy Techniques and Applications
- Keywords
- nuclear pore complex, Arabidopsis thaliana, nucleoporins, nuclear envelope, NUP155, HOS1, cryo-electron tomography, root protoplasts
- MeSH terms
- Humans, Arabidopsis, Cryoelectron Microscopy, Nuclear Pore, Nuclear Pore Complex Proteins, Arabidopsis Proteins
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