Cited by 148Malaria Research and ControlImmunotherapy and Immune ResponsesNanoparticle-Based Drug Delivery

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A malaria vaccine adjuvant based on recombinant antigen binding to liposomes

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Huang, Wei-Chiao, Deng, Bingbing, Lin, Cuiyan, Carter, Kevin A., Geng, Jumin, Razi, Aida, He, Xuedan, Chitgupi, Upendra, Federizon, Jasmin, Sun, Boyang, Long, Carole A., Ortega, Joaquin, Dutta, Sheetij, King, C. Richter, Miura, Kazutoyo, Lee, Shwu-Maan, Lovell, Jonathan F.·2018·Nature Nanotechnology

DOI 10.1038/s41565-018-0271-3PMC PMC6286227

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Abstract

Pfs25 is a malaria transmission-blocking vaccine antigen candidate, but its apparently limited immunogenicity in humans has hindered clinical development. Here, we show that recombinant, polyhistidine-tagged (his-tagged) Pfs25 can be mixed at the time of immunization with pre-formed liposomes containing cobalt porphyrin-phospholipid, resulting in spontaneous nanoliposome antigen particleization (SNAP). Antigens are stably presented in uniformly orientated display via his-tag insertion in the cobalt porphyrin-phospholipid bilayer, without covalent modification or disruption of antigen conformation. SNAP immunization of mice and rabbits is well tolerated with minimal local reactogenicity, and results in orders-of-magnitude higher functional antibody generation compared with other 'mix-and-inject' adjuvants. Serum-stable antigen binding during transit to draining lymph nodes leads to enhanced antigen uptake by phagocytic antigen-presenting cells, with subsequent generation of long-lived, antigen-specific plasma cells. Seamless multiplexing with four additional his-tagged Plasmodium falciparum polypeptides induces strong and balanced antibody production, illustrating the simplicity of developing multistage particulate vaccines with SNAP immunization.

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Authors: Huang, W.-C., Deng, B., Lin, C., Carter, K. A., Geng, J., Razi, A., He, X., Chitgupi, U., Federizon, J., Sun, B., Long, C. A., Ortega, J., Dutta, S., King, C. R., Miura, K., Lee, S.-M., & Lovell, J. F.
Affiliations: University at Buffalo, State University of New York (US)
National Institute of Allergy and Infectious Diseases (US)
McGill University (CA)
Walter Reed Army Institute of Research (US)
Corresponding author: Lovell, Jonathan F.
ORCID: Huang, Wei-Chiao
Lin, Cuiyan
Razi, Aida
He, Xuedan
Chitgupi, Upendra
Sun, Boyang
Long, Carole A.
Dutta, Sheetij
King, C. Richter
Miura, Kazutoyo
Lee, Shwu-Maan
Lovell, Jonathan F.
Venue: Nature Nanotechnology
Publisher: Springer Science and Business Media LLC
Published: 2018-10-08
Volume: 13
Issue: 12
Pages: 1174-1181
ISSN: 1748-3387 (ISSN-L)
1748-3395
DOI: 10.1038/s41565-018-0271-3
PMC: PMC6286227
OpenAlex: W2894881231
Cited by: 148
References: 51
Citations by year: 2026: 10 · 2025: 18 · 2024: 20 · 2023: 19 · 2022: 20 · 2021: 19 · 2020: 23 · 2019: 17 · 2018: 2
FWCI: 14.52
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Licence: www.springer.com/tdm (tdm)
Locations: Nature Nanotechnology (publishedVersion)
PubMed (publishedVersion)
Europe PMC (PubMed Central) (acceptedVersion)
PubMed Central (acceptedVersion)
Funders: National Institutes of Health (DP5OD017898)
National Institute of Allergy and Infectious Diseases (R21AI122964)
Topics: Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Malaria Research and Control
Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
Keywords: malaria vaccine, vaccine adjuvants, Pfs25, transmission-blocking vaccine, Plasmodium falciparum, antigen-presenting cells, plasma cells, functional antibodies, particulate vaccines
MeSH terms: RAW 264.7 Cells, Adjuvants, Immunologic, Animals, Antibody Formation, Antigens, Protozoan, Female, Humans, Immunization, Liposomes, Plasmodium falciparum, Rabbits, Recombinant Proteins, Protozoan Proteins, Malaria, Falciparum, Malaria Vaccines, Mice

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A malaria vaccine adjuvant based on recombinant antigen binding to liposomes

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