The DOI matches its Crossref record; 41 of the 41 references it cites were found in a registry or an index.
Hepatocytes direct the formation of a pro-metastatic niche in the liver
Open accessAbstract
The liver is the most common site of metastatic disease 1. Although this metastatic tropism may reflect the mechanical trapping of circulating tumour cells, liver metastasis is also dependent, at least in part, on the formation of a 'pro-metastatic' niche that supports the spread of tumour cells to the liver 2,3. The mechanisms that direct the formation of this niche are poorly understood. Here we show that hepatocytes coordinate myeloid cell accumulation and fibrosis within the liver and, in doing so, increase the susceptibility of the liver to metastatic seeding and outgrowth. During early pancreatic tumorigenesis in mice, hepatocytes show activation of signal transducer and activator of transcription 3 (STAT3) signalling and increased production of serum amyloid A1 and A2 (referred to collectively as SAA). Overexpression of SAA by hepatocytes also occurs in patients with pancreatic and colorectal cancers that have metastasized to the liver, and many patients with locally advanced and metastatic disease show increases in circulating SAA. Activation of STAT3 in hepatocytes and the subsequent production of SAA depend on the release of interleukin 6 (IL-6) into the circulation by non-malignant cells. Genetic ablation or blockade of components of IL-6-STAT3-SAA signalling prevents the establishment of a pro-metastatic niche and inhibits liver metastasis. Our data identify an intercellular network underpinned by hepatocytes that forms the basis of a pro-metastatic niche in the liver, and identify new therapeutic targets.
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“Markedly, in mouse fetal livers, STAT3 was not activated (Figure S6D) and canonical STAT3 target genes 31,32 were not induced (Figure S6E).”
From Kupffer-cell-derived IL-6 is repurposed for hepatocyte dedifferentiation via activating progenitor genes from injury-specific enhancers (2023) · cites this paper as [31]
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Subramanian, A., Tamayo, P., Mootha, V. K., Mukherjee, S., Ebert, B. L., Gillette, M. A., Paulovich, A., Pomeroy, S. L., Golub, T. R., Lander, E. S., et al. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0506580102 · Cited by 53,625
Cytoscape: A software Environment for integrated models of biomolecular interaction networks
Shannon, P., Markiel, A., Ozier, O., Baliga, N. S., Wang, J. T., Ramage, D., Amin, N., Schwikowski, B., & Ideker, T. (2003) · Genome Research · doi:10.1101/gr.1239303 · Cited by 51,888
Gene Ontology: tool for the unification of biology
Ashburner, M., Ball, C. A., Blake, J. A., Botstein, D., Butler, H., Cherry, J. M., Davis, A. P., Dolinski, K., Dwight, S. S., Eppig, J. T., et al. (2000) · Nature Genetics · doi:10.1038/75556 · Cited by 43,206
Primer3—new capabilities and interfaces
Untergasser, A., Cutcutache, I., Kõressaar, T., Ye, J., Faircloth, B., Remm, M., & Rozen, S. (2012) · Nucleic Acids Research · doi:10.1093/nar/gks596 · Cited by 9,165
Spencer, P. M., Cameron, J. L., & Lin, L. (2000) · Cited by 7,202
Bindea, G., Mlecnik, B., Hackl, H., Charoentong, P., Tosolini, M., Kirilovsky, A., Fridman, W.-H., Pagès, F., Trajanoski, Z., & Galon, J. (2009) · Bioinformatics · doi:10.1093/bioinformatics/btp101 · Cited by 6,280
Tumour exosome integrins determine organotropic metastasis
Hoshino, A., Costa-Silva, B., Shen, T.-L., Rodrigues, G., Hashimoto, A., Tesic Mark, M., Molina, H., Kohsaka, S., Di Giannatale, A., Ceder, S., et al. (2015) · Nature · doi:10.1038/nature15756 · Cited by 5,101
Pancreatic cancer exosomes initiate pre-metastatic niche formation in the liver
Costa-Silva, B., Aiello, N. M., Ocean, A. J., Singh, S., Zhang, H., Thakur, B. K., Becker, A., Hoshino, A., Mark, M. T., Molina, H., et al. (2015) · Nature Cell Biology · doi:10.1038/ncb3169 · Cited by 2,692
Hingorani, S. R., Wang, L., Multani, A. S., Combs, C., Deramaudt, T. B., Hruban, R. H., Rustgi, A. K., Chang, S., & Tuveson, D. A. (2005) · Cancer Cell · doi:10.1016/j.ccr.2005.04.023 · Cited by 2,609
Hidalgo, M. (2010) · New England Journal of Medicine · doi:10.1056/nejmra0901557 · Cited by 2,570
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Molecular principles of metastasis: a hallmark of cancer revisited
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Metadata
- Authors
- Lee, J. W., Stone, M. L., Porrett, P. M., Thomas, S. K., Komar, C. A., Li, J. H., Delman, D., Graham, K., Gladney, W. L., Hua, X., Black, T. A., Chien, A. L., Majmundar, K. S., Thompson, J. C., Yee, S. S., O’Hara, M. H., Aggarwal, C., Xin, D., Shaked, A., Gao, M., Liu, D., Borad, M. J., Ramanathan, R. K., Carpenter, E. L., Ji, A., de Beer, M. C., de Beer, F. C., Webb, N. R., & Beatty, G. L.
- Affiliations
- Abramson Cancer Center (US)
- University of Pennsylvania (US)
- University of Georgia (US)
- Mayo Clinic Comprehensive Cancer Center (Arizona) (US)
- Merck & Co., Inc., Rahway, NJ, USA (United States) (US)
- University of Kentucky (US)
- Corresponding author
- Beatty, Gregory L.
- ORCID
- Lee, Jae W.
- Stone, Meredith L.
- Porrett, Paige M.
- Thomas, Stacy K.
- Li, Joey H.
- Graham, Kathleen
- Gladney, Whitney L.
- Chien, Austin L.
- Thompson, Jeffrey C.
- Yee, Stephanie S.
- O’Hara, Mark H.
- Aggarwal, Charu
- Xin, Dong
- Shaked, Abraham
- Gao, Mingming
- Liu, Dexi
- Borad, Mitesh J.
- Carpenter, Erica L.
- Ji, Ailing
- de Beer, Maria C.
- de Beer, Frederick C.
- Webb, Nancy R.
- Beatty, Gregory L.
- Venue
- Nature
- Published
- 2019-03-06
- Volume
- 567
- Issue
- 7747
- Pages
- 249-252
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- PMC
- PMC6430113
- OpenAlex
- W2919508314
- Cited by
- 402
- References
- 41
- Citations by year
- 2026: 30 · 2025: 55 · 2024: 55 · 2023: 64 · 2022: 54 · 2021: 58 · 2020: 57 · 2019: 26
- FWCI
- 20.38
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- Locations
- Nature (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- RePEc: Research Papers in Economics (submittedVersion)
- Funders
- University of Pennsylvania
- National Heart, Lung, and Blood Institute (R01HL134731, T32HL007439)
- National Cancer Institute (T32CA009140, F30CA196106, R01CA197916, P30CA016520)
- National Institute of Diabetes and Digestive and Kidney Diseases (P30DK050306)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Cytokine Signaling Pathways and Interactions
- Health Sciences › Medicine › Oncology › Cancer Cells and Metastasis
- Life Sciences › Immunology and Microbiology › Immunology › Immune cells in cancer
- Keywords
- liver metastasis, hepatocytes, STAT3 signaling, serum amyloid A, interleukin-6, pancreatic cancer, colorectal cancer, liver fibrosis, tumor microenvironment, metastatic tropism
- MeSH terms
- Serum Amyloid A Protein, Animals, Female, Liver, Liver Neoplasms, Male, Neoplasm Metastasis, Pancreatic Neoplasms, Colorectal Neoplasms, Interleukin-6, Carcinoma, Pancreatic Ductal, Hepatocytes, STAT3 Transcription Factor, Mice, Tumor Microenvironment
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