The DOI matches its Crossref record; 68 of the 68 references it cites were found in a registry or an index.
Engineering transplantable jejunal mucosal grafts using patient-derived organoids from children with intestinal failure
Open accessPublished version of a preprint
Abstract
Intestinal failure, following extensive anatomical or functional loss of small intestine, has debilitating long-term consequences for children 1. The priority of patient care is to increase the length of functional intestine, particularly the jejunum, to promote nutritional independence 2. Here we construct autologous jejunal mucosal grafts using biomaterials from pediatric patients and show that patient-derived organoids can be expanded efficiently in vitro. In parallel, we generate decellularized human intestinal matrix with intact nanotopography, which forms biological scaffolds. Proteomic and Raman spectroscopy analyses reveal highly analogous biochemical profiles of human small intestine and colon scaffolds, indicating that they can be used interchangeably as platforms for intestinal engineering. Indeed, seeding of jejunal organoids onto either type of scaffold reliably reconstructs grafts that exhibit several aspects of physiological jejunal function and that survive to form luminal structures after transplantation into the kidney capsule or subcutaneous pockets of mice for up to 2 weeks. Our findings provide proof-of-concept data for engineering patient-specific jejunal grafts for children with intestinal failure, ultimately aiding in the restoration of nutritional autonomy.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The check wasn’t confident either way, so it gives no verdict — never disputed.
In the citing paper
“Similarly, pancreatic islets cultured in an artificial ECM could be successfully engrafted into either streptozocin-induced or autoimmune-driven diabetic mouse models, rescuing insulin production and reversing hyperglycemia147.”
From Organoids (2022) · cites this paper as [147]
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
QuPath: Open source software for digital pathology image analysis
Bankhead, P., Loughrey, M. B., Fernández, J. A., Dombrowski, Y., McArt, D. G., Dunne, P. D., McQuaid, S., Gray, R. T., Murray, L. J., Coleman, H. G., et al. (2017) · Scientific Reports · doi:10.1038/s41598-017-17204-5 · Cited by 8,908
Sato, T., Stange, D. E., Ferrante, M., Vries, R. G. J., van Es, J. H., van den Brink, S., van Houdt, W. J., Pronk, A., van Gorp, J., Siersema, P. D., et al. (2011) · Gastroenterology · doi:10.1053/j.gastro.2011.07.050 · Cited by 3,753
Organoid Models and Applications in Biomedical Research
Xinaris, C., Brizi, V., & Remuzzi, G. (2015) · The Nephron journals/Nephron journals · doi:10.1159/000433566 · Cited by 3,519
Raman Spectroscopy of Biological Tissues
Movasaghi, Z., Rehman, S., & Rehman, I. U. (2007) · Applied Spectroscopy Reviews · doi:10.1080/05704920701551530 · Cited by 2,518
Tissue-engineered autologous bladders for patients needing cystoplasty
Atala, A., Bauer, S. B., Söker, S., Yoo, J. J., & Retik, A. B. (2006) · The Lancet · doi:10.1016/s0140-6736(06)68438-9 · Cited by 1,857
Uygun, B. E., Soto-Gutierrez, A., Yagi, H., Izamis, M.-L., Guzzardi, M. A., Shulman, C., Milwid, J., Kobayashi, N., Tilles, A., Berthiaume, F., et al. (2010) · Nature Medicine · doi:10.1038/nm.2170 · Cited by 1,370
Naba, A., Clauser, K. R., Hoersch, S., Liu, H., Carr, S. A., & Hynes, R. O. (2012) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m111.014647 · Cited by 1,354
Somatic coding mutations in human induced pluripotent stem cells
Gore, A., Li, Z., Fung, H.-L., Young, J. E., Agarwal, S., Antosiewicz-Bourget, J., Canto, I., Giorgetti, A., Israel, M. A., Kiskinis, E., et al. (2011) · Nature · doi:10.1038/nature09805 · Cited by 1,273
Limbal Stem-Cell Therapy and Long-Term Corneal Regeneration
Rama, P., Matuška, S., Paganoni, G., Spinelli, A. M., Luca, M. D., & Pellegrini, G. (2010) · New England Journal of Medicine · doi:10.1056/nejmoa0905955 · Cited by 1,142
Regeneration and orthotopic transplantation of a bioartificial lung
Ott, H. C., Clippinger, B., Conrad, C., Schuetz, C., Pomerantseva, I., Ikonomou, L., Kotton, D., & Vacanti, J. P. (2010) · Nature Medicine · doi:10.1038/nm.2193 · Cited by 1,090
Cited by
Sorted by citations
Zhao, Z., Chen, X., Dowbaj, A. M., Sljukic, A., Bratlie, K., Lin, L., Fong, E. L. S., Balachander, G. M., Chen, Z., Soragni, A., et al. (2022) · Nature Reviews Methods Primers · doi:10.1038/s43586-022-00174-y · Cited by 1,082
Organoids: The current status and biomedical applications
Yang, S., Hu, H., Kung, H., Zou, R.-Q., Dai, Y.-S., Hu, Y.-F., Wang, T., Lv, T., Yu, J., & Li, F. (2023) · MedComm · doi:10.1002/mco2.274 · Cited by 300
An organoid-based organ-repurposing approach to treat short bowel syndrome
Sugimoto, S., Kobayashi, E., Fujii, M., Ohta, Y., Arai, K., Matano, M., Ishikawa, K., Miyamoto, K., Toshimitsu, K., Takahashi, S., et al. (2021) · Nature · doi:10.1038/s41586-021-03247-2 · Cited by 175
Research progress in decellularized extracellular matrix-derived hydrogels
Zhang, W., Du, A., Liu, S., Lv, M., & Chen, S. (2021) · Regenerative Therapy · doi:10.1016/j.reth.2021.04.002 · Cited by 176
Organoids in gastrointestinal diseases: From experimental models to clinical translation
Günther, C., Winner, B., Neurath, M. F., & Stappenbeck, T. S. (2022) · Gut · doi:10.1136/gutjnl-2021-326560 · Cited by 145
Metadata
- Authors
- Meran, L., Massie, I., Campinoti, S., Weston, A. E., Gaifulina, R., Tullie, L., Faull, P., Orford, M., Kucharska, A., Baulies, A., Novellasdemunt, L., Angelis, N., Hirst, E., König, J., Tedeschi, A. M., Pellegata, A. F., Eli, S., Snijders, A. P., Collinson, L., Thapar, N., Thomas, G. M. H., Eaton, S., Bonfanti, P., De Coppi, P., & Li, V. S. W.
- Affiliations
- The Francis Crick Institute (GB)
- University College London (GB)
- Children's Health Queensland Hospital and Health Service (AU)
- Corresponding author
- Li, Vivian S. W.
- ORCID
- Meran, Laween
- Massie, Isobel
- Campinoti, Sara
- Weston, Anne E.
- Gaifulina, Riana
- Tullie, Lucinda
- Faull, Peter
- Orford, Michael
- Baulies, Anna
- Novellasdemunt, Laura
- Angelis, Nikolaos
- Hirst, Elizabeth
- Pellegata, Alessandro Filippo
- Snijders, Ambrosius P.
- Collinson, Lucy
- Thapar, Nikhil
- Thomas, Geraint M. H.
- Eaton, Simon
- Bonfanti, Paola
- De Coppi, Paolo
- Li, Vivian S. W.
- Venue
- Nature Medicine
- Published
- 2020-09-07
- Volume
- 26
- Issue
- 10
- Pages
- 1593-1601
- ISSN
- 1078-8956 (ISSN-L)
- 1546-170X
- PMC
- PMC7116539
- OpenAlex
- W3083160231
- Cited by
- 143
- References
- 66
- Citations by year
- 2026: 15 · 2025: 13 · 2024: 21 · 2023: 37 · 2022: 34 · 2021: 18 · 2020: 5
- FWCI
- 21.48
- Type
- journal article
- Language
- en
- Open access
- green
- doi.org
- Locations
- Nature Medicine (publishedVersion)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Cineca Institutional Research Information System (Tor Vergata University) (submittedVersion)
- UCL Discovery (University College London) (submittedVersion)
- Virtual Community of Pathological Anatomy (University of Castilla La Mancha) (submittedVersion)
- Funders
- EC | Horizon 2020 Framework Programme (668294)
- Cancer Research UK (FC001105)
- RCUK | Medical Research Council (MRC (FC001105)
- Wellcome Trust (FC001105)
- Francis Crick Institute
- Topics
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Physical Sciences › Materials Science › Biomaterials › Electrospun Nanofibers in Biomedical Applications
- Keywords
- intestinal failure, patient-derived organoids, biological scaffolds, autologous tissue transfer, nanotopography, pediatric patients, organoid expansion, graft reconstruction, kidney capsule transplantation, Raman spectroscopy, proteomics
- MeSH terms
- Proof of Concept Study, Animals, Cell Differentiation, Cells, Cultured, Child, Extracellular Matrix, Female, Humans, Intestinal Diseases, Intestinal Mucosa, Jejunum, Mice, Transgenic, Organoids, Swine, Mice, SCID, Mice, Inbred NOD, Enterocytes, Tissue Engineering, Cell Proliferation, Mice, Tissue Scaffolds, Precision Medicine, HEK293 Cells, Primary Cell Culture, Human Umbilical Vein Endothelial Cells
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
