Cell-type-specific and projection-specific brain-wide reconstruction of single neurons
Abstract
We developed a dual-adeno-associated-virus expression system that enables strong and sparse labeling of individual neurons with cell-type and projection specificity. We demonstrated its utility for whole-brain reconstruction of midbrain dopamine neurons and striatum-projecting cortical neurons. We further extended the labeling method for rapid reconstruction in cleared thick brain sections and simultaneous dual-color labeling. This labeling system may facilitate the process of generating mesoscale single-neuron projectomes of mammalian brains.
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By combining fMOST and the dual-AAV sparse labeling system, their strategy ensures that they are precisely tracing genetically defined individual serotonin neurons and their projections across the whole brain.
“By combining fMOST (Gong et al., 2016) and the dual-AAV sparse labeling system (Lin et al., 2018), our strategy ensures that we are precisely tracing genetically defined individual serotonin neurons and their projections across the whole brain.”
From Single-cell transcriptomes and whole-brain projections of serotonin neurons in the mouse dorsal and median raphe nuclei (2019) · cites this paper as Lin et al., 2018
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References 34 · Cited by 132
- Authors
- Lin, R., Wang, R., Yuan, J., Feng, Q., Zhou, Y., Zeng, S., Ren, M., Jiang, S., Ni, H., Zhou, C., Gong, H., & Luo, M.
- Affiliations
- Peking University (CN)
- Wuhan National Laboratory for Optoelectronics (CN)
- National Institute of Biological Sciences, Beijing (CN)
- Corresponding author
- Gong, Hui
- Luo, Minmin
- Venue
- Nature Methods
- Published
- 2018-11-19
- Volume
- 15
- Issue
- 12
- Pages
- 1033-1036
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- OpenAlex
- W2900358948
- Cited by
- 132
- References
- 34
- Citations by year
- 2026: 16 · 2025: 18 · 2024: 12 · 2023: 9 · 2022: 17 · 2021: 22 · 2020: 22 · 2019: 15 · 2012: 1
- FWCI
- 6.71
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Methods (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Virus-based gene therapy research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Cell Image Analysis Techniques
- Keywords
- single-neuron reconstruction, midbrain dopaminergic neurons, brain clearing, mouse brain
- MeSH terms
- Dependovirus, Animals, Brain, Brain Mapping, Cells, Cultured, Cerebral Cortex, Genetic Vectors, Mice, Inbred C57BL, Neural Pathways, Gene Transfer Techniques, Mice, Dopaminergic Neurons
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