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Cited by 48Advanced Fluorescence Microscopy TechniquesBiochemical and Structural CharacterizationBiotin and Related Studies

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A fluorogenic chemically induced dimerization technology for controlling, imaging and sensing protein proximity

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Bottone, Sara, Joliot, Octave, Cakil, Zeyneb Vildan, El Hajji, Lina, Rakotoarison, Louise-Marie, Boncompain, Gaelle, Perez, Franck, Gautier, Arnaud·2023·Nature Methods
DOI 10.1038/s41592-023-01988-8

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Abstract

Molecular tools enabling the control and observation of the proximity of proteins are essential for studying the functional role of physical distance between two proteins. Here we present CATCHFIRE (chemically assisted tethering of chimera by fluorogenic-induced recognition), a chemically induced proximity technology with intrinsic fluorescence imaging and sensing capabilities. CATCHFIRE relies on genetic fusion to small dimerizing domains that interact upon addition of fluorogenic inducers of proximity that fluoresce upon formation of the ternary assembly, allowing real-time monitoring of the chemically induced proximity. CATCHFIRE is rapid and fully reversible and allows the control and tracking of protein localization, protein trafficking, organelle transport and cellular processes, opening new avenues for studying or controlling biological processes with high spatiotemporal resolution. Its fluorogenic nature allows the design of a new class of biosensors for the study of processes such as signal transduction and apoptosis.

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Metadata

Authors
Bottone, S., Joliot, O., Cakil, Z. V., El Hajji, L., Rakotoarison, L.-M., Boncompain, G., Perez, F., & Gautier, A.
Affiliations
  • Centre National de la Recherche Scientifique (FR)
  • Université Paris Sciences et Lettres (FR)
Corresponding author
  • Perez, Franck
  • Gautier, Arnaud
ORCID
Venue
Nature Methods
Publisher
Springer Science and Business Media LLC
Published
2023-08-28
Volume
20
Issue
10
Pages
1553-1562
ISSN
  • 1548-7091 (ISSN-L)
  • 1548-7105
DOI
10.1038/s41592-023-01988-8
OpenAlex
W4386216012
Cited by
48
References
4
Citations by year
2026: 18 · 2025: 17 · 2024: 9 · 2023: 4
FWCI
5.95
Type
journal article
Language
en
Open access
Licence
Locations
  • Nature Methods (publishedVersion)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • HAL (submittedVersion, cc-by-nc-nd)
Funders
  • Agence Nationale de la Recherche (ANR-19-CE13-0026, ANR-10-IDEX-0001, ANR‐19, ANR-19-CE13-0006, ANR-10-IDEX0001-02., ANR-10-IDEX, ANR-20-CE14-0017, ANR-11-LABX-0038, 11-LBX-0038, ANR10IDEX000102 PSL, ANR-19-CE13-0002, ANR-10, IDEX-0001-02)
  • Fondation pour la Recherche Médicale (ANR-10-IDEX-0001-02 PSL, EQU201903007925)
  • Institut Universitaire de France
  • Sorbonne Université
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Structural Characterization
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Biotin and Related Studies
Keywords
turn-on fluorescence, protein trafficking, organelle transport, protein localization, biosensors, signal transduction, apoptosis
MeSH terms
Fluorescent Dyes, Humans, Microscopy, Fluorescence, Proteins, Biosensing Techniques, Protein Transport, Protein Multimerization

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