The DOI matches its Crossref record; 6 of the 6 references it cites were found in a registry or an index.
Cryo-EM structures of complex I from mouse heart mitochondria in two biochemically defined states
Open accessAbstract
Complex I (NADH:ubiquinone oxidoreductase) uses the reducing potential of NADH to drive protons across the energy-transducing inner membrane and power oxidative phosphorylation in mammalian mitochondria. Recent cryo-EM analyses have produced near-complete models of all 45 subunits in the bovine, ovine and porcine complexes and have identified two states relevant to complex I in ischemia–reperfusion injury. Here, we describe the 3.3-Å structure of complex I from mouse heart mitochondria, a biomedically relevant model system, in the ‘active’ state. We reveal a nucleotide bound in subunit NDUFA10, a nucleoside kinase homolog, and define mechanistically critical elements in the mammalian enzyme. By comparisons with a 3.9-Å structure of the ‘deactive’ state and with known bacterial structures, we identify differences in helical geometry in the membrane domain that occur upon activation or that alter the positions of catalytically important charged residues. Our results demonstrate the capability of cryo-EM analyses to challenge and develop mechanistic models for mammalian complex I. Cryo-EM analyses of mitochondrial complex I (NADH:ubiquinone oxidoreductase) isolated from mouse heart allow comparisons between the active and deactive states and provide new mechanistic insights into this important complex.
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Crystal structure of the entire respiratory complex I
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Structure of the Hydrophilic Domain of Respiratory Complex I from Thermus thermophilus
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Cardioprotection by S-nitrosation of a cysteine switch on mitochondrial complex I
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Structure of the membrane domain of respiratory complex I
Efremov, R. G., & Sazanov, L. A. (2011) · Nature · doi:10.1038/nature10330 · Cited by 370
Subunit NDUFV3 is present in two distinct isoforms in mammalian complex I
Bridges, H. R., Mohammed, K., Harbour, M. E., & Hirst, J. (2017) · Biochimica et Biophysica Acta (BBA) - Bioenergetics · doi:10.1016/j.bbabio.2016.12.001 · Cited by 38
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Self-citationStructure of mycobacterial respiratory complex I
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Metadata
- Authors
- Agip, A.-N. A., Blaza, J. N., Bridges, H. R., Viscomi, C., Rawson, S., Muench, S. P., & Hirst, J.
- Venue
- Nature Structural & Molecular Biology
- Published
- 2018-06-18
- Volume
- 25
- Issue
- 7
- Pages
- 548-556
- ISSN
- 1545-9985 (ISSN-L)
- 1545-9993
- OpenAlex
- W2806917274
- Cited by
- 228
- References
- 6
- FWCI
- 10.12
- Type
- journal article
- Language
- en
- Open access
- green
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- Agip, A.-N. A., Blaza, J. N., Bridges, H. R., Viscomi, C., Rawson, S., Muench, S. P., & Hirst, J. (2018). Cryo-EM structures of complex I from mouse heart mitochondria in two biochemically defined states. Nature Structural & Molecular Biology. https://doi.org/10.1038/s41594-018-0073-1
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