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Cited by 121Pharmaceutical and Antibiotic Environmental ImpactsMicrobial bioremediation and biosurfactantsEnvironmental Chemistry and Analysis

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Features of diclofenac biodegradation by Rhodococcus ruber IEGM 346

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Ivshina, Irina B., Tyumina, Elena A., Kuzmina, Maria V., Vikhareva, Elena V.·2019·Scientific Reports
DOI 10.1038/s41598-019-45732-9PMC PMC6591480
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Abstract

This study investigated the ability of rhodococci to biodegrade diclofenac (DCF), one of the polycyclic non-steroidal anti-inflammatory drugs (NSAIDs) most frequently detected in the environment. Rhodococcus ruber strain IEGM 346 capable of complete DCF biodegradation (50 µg/L) over 6 days was selected. It is distinguished by the ability to degrade DCF at high (50 mg/L) concentrations unlike other known biodegraders. The DCF decomposition process was accelerated by adding glucose and due to short-term cell adaptation to 5 µg/L DCF. The most typical responses to DCF exposure observed were the changed ζ-potential of bacterial cells; increased cell hydrophobicity and total cell lipid content; multi-cellular conglomerates formed; and the changed surface-to-volume ratio. The obtained findings are considered as mechanisms of rhodococcal adaptation and hence their increased resistance to toxic effects of this pharmaceutical pollutant. The proposed pathways of bacterial DCF metabolisation were described. The data confirming the C-N bond cleavage and aromatic ring opening in the DCF structure were obtained.

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  • Rhodococcus has significant potential for treating various organic and inorganic compounds, including PAHs, phenolic compounds, fungicides, pharmaceuticals, phthalates, steroids, dyes, and heavy metals.

    “Rhodococcus, a genus of actinomycetes, has significant potential for treating various organic and inorganic compounds, including PAHs, phenolic compounds, fungicides, pharmaceuticals, phthalates, steroids, dyes, and heavy metals [32,163,299].”

    From Harnessing bacterial consortia for effective bioremediation: targeted removal of heavy metals, hydrocarbons, and persistent pollutants (2025) · cites this paper as [163]

    The Introduction describes Rhodococcus as having high bioremediation potential and the ability to biodegrade pharmaceuticals; the Discussion points to pharmaceutical sewage treatment. Our copy does not establish the claimed treatment potential for the full list of compounds, particularly dyes and heavy metals. (LLM, conf=0.86)

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Metadata

Authors
Ivshina, I. B., Tyumina, E. A., Kuzmina, M. V., & Vikhareva, E. V.
Affiliations
  • Perm State University (RU)
  • Perm State Pharmaceutical Academy (RU)
ORCID
Venue
Scientific Reports
Publisher
Springer Science and Business Media LLC
Published
2019-06-24
Volume
9
Issue
1
Pages
9159-9159
ISSN
2045-2322 (ISSN-L)
DOI
10.1038/s41598-019-45732-9
PMC
PMC6591480
OpenAlex
W2953581678
Cited by
121
References
1
Citations by year
2026: 22 · 2025: 17 · 2024: 13 · 2023: 26 · 2022: 18 · 2021: 12 · 2020: 12 · 2019: 1
FWCI
3.16
Type
journal article
Language
en
Open access
Licence
Locations
  • Scientific Reports (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Funders
  • Russian Foundation for Basic Research (17-44-590567)
  • The State assignment
Topics
  • Physical Sciences › Environmental Science › Pollution › Pharmaceutical and Antibiotic Environmental Impacts
  • Physical Sciences › Environmental Science › Pollution › Microbial bioremediation and biosurfactants
  • Physical Sciences › Environmental Science › Environmental Chemistry › Environmental Chemistry and Analysis
Keywords
diclofenac, Rhodococcus ruber, biodegradation, nonsteroidal anti-inflammatory drugs, pharmaceutical pollutants, bacterial adaptation, cell surface hydrophobicity, zeta potential, cell aggregation, C–N bond cleavage, aromatic ring opening, biodegradation pathways, glucose induction
MeSH terms
Biodegradation, Environmental, Diclofenac, Dose-Response Relationship, Drug, Rhodococcus, Water Pollutants, Chemical, Drug Resistance, Bacterial, Hydrophobic and Hydrophilic Interactions

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