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Cited by 26Virology and Viral DiseasesViral Infections and VectorsViral Infections and Outbreaks Research

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Establishment of an RNA polymerase II-driven reverse genetics system for Nipah virus strains from Malaysia and Bangladesh

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Griffin, Bryan D., Leung, Anders, Chan, Mable, Warner, Bryce M., Ranadheera, Charlene, Tierney, Kevin, Audet, Jonathan, Frost, Kathy L., Safronetz, David, Embury-Hyatt, Carissa, Booth, Stephanie A., Kobasa, Darwyn·2019·Scientific Reports
DOI 10.1038/s41598-019-47549-yPMC PMC6671980
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Abstract

Nipah virus (NiV) has emerged as a highly lethal zoonotic paramyxovirus that is capable of causing a febrile encephalitis and/or respiratory disease in humans for which no vaccines or licensed treatments are currently available. There are two genetically and geographically distinct lineages of NiV: NiV-Malaysia (NiV-M), the strain that caused the initial outbreak in Malaysia, and NiV-Bangladesh (NiV-B), the strain that has been implicated in subsequent outbreaks in India and Bangladesh. NiV-B appears to be both more lethal and have a greater propensity for person-to-person transmission than NiV-M. Here we describe the generation and characterization of stable RNA polymerase II-driven infectious cDNA clones of NiV-M and NiV-B. In vitro , reverse genetics-derived NiV-M and NiV-B were indistinguishable from a wildtype isolate of NiV-M, and both viruses were pathogenic in the Syrian hamster model of NiV infection. We also describe recombinant NiV-M and NiV-B with enhanced green fluorescent protein (EGFP) inserted between the G and L genes that enable rapid and sensitive detection of NiV infection in vitro . This panel of molecular clones will enable studies to investigate the virologic determinants of henipavirus pathogenesis, including the pathogenic differences between NiV-M and NiV-B, and the high-throughput screening of candidate therapeutics.

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Metadata

Authors
Griffin, B. D., Leung, A., Chan, M., Warner, B. M., Ranadheera, C., Tierney, K., Audet, J., Frost, K. L., Safronetz, D., Embury-Hyatt, C., Booth, S. A., & Kobasa, D.
Affiliations
Public Health Agency of Canada (CA)
Corresponding author
Kobasa, Darwyn
ORCID
Venue
Scientific Reports
Publisher
Springer Science and Business Media LLC
Published
2019-08-01
Volume
9
Issue
1
Pages
11171-11171
ISSN
2045-2322 (ISSN-L)
DOI
10.1038/s41598-019-47549-y
PMC
PMC6671980
OpenAlex
W2965062769
Cited by
26
References
1
Citations by year
2026: 3 · 2025: 4 · 2024: 3 · 2023: 5 · 2021: 4 · 2020: 4 · 2019: 3
FWCI
2.09
Type
journal article
Language
en
Open access
Licence
Locations
  • Scientific Reports (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Funders
Public Health Agency of Canada
Topics
  • Health Sciences › Medicine › Epidemiology › Virology and Viral Diseases
  • Health Sciences › Medicine › Infectious Diseases › Viral Infections and Vectors
  • Health Sciences › Medicine › Infectious Diseases › Viral Infections and Outbreaks Research
Keywords
Nipah virus, infectious clone, Syrian hamster model, zoonotic paramyxovirus, human-to-human transmission
MeSH terms
Animals, Bangladesh, Disease Outbreaks, Humans, Malaysia, Mesocricetus, RNA Polymerase II, Nipah Virus, Henipavirus Infections, Reverse Genetics

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