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Cited by 91Berberine and alkaloids researchTraditional Chinese Medicine AnalysisAgricultural and Biological Research

Optimized microwave assisted extraction (MAE) of alkaloids and polyphenols from Berberis roots using multiple-component analysis

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Belwal, Tarun, Pandey, Aseesh, Bhatt, Indra D., Rawal, Ranbeer S.·2020·Scientific Reports
DOI 10.1038/s41598-020-57585-8PMC PMC6976575
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Abstract

Berberis, one of the major sources of berberine and polyphenols, is widely accepted genus for its medicinal properties. The inclusion of these phytochemicals in different health formulations has widened its scope in pharmaceuticals and nutraceuticals. In the present study, multi-component analysis (MCA) has been used to extract these nutraceutical compounds from Berberis jaeschkeana roots under microwave-assisted extraction (MAE) conditions. To determine the optimum extraction condition, different factors, including, microwave power, sample to solvent ratio, irradiation time, solvent pH and solvent concentration were tested under 42 experiments. The MCA includes, Plackett-Burmen and Central Composite Design and analyzes model fitness, regression coefficient (β), analysis of variance (ANOVA) and 3D response curve. The results showed significant model fitness and involvement of linear, quadratic and interactive effect of different factors. Under optimized MAE condition, [i.e. 1 g of sample extracted through 70 mL of a solution (100% methanol pH 2.0), provided microwave power of 598 W for 2 min of irradiation time], the berberine and palmatine contents were recorded as 46.38 mg g−1 and 20.54 mg g−1 respectively. Under optimized condition, the yield of alkaloids were found closer to the models’ predicted value. Similarly, total phenolic content and antioxidant activities were also found closer to the models’ predicted value. To test the suitability of the optimized MAE condition for other species i.e.,Berberis asiatica, extraction of alkaloids and polyphenolics was conducted and recorded higher yield to the previous records. Moreover, under optimum extraction condition, six and seven polyphenolic compounds from B. jaeschkeana and B. asiatica were quantified respectively. The proposed MAE optimization design using MCA contributes towards faster and greener extraction of alkaloids and polyphenolics with higher yield. Moreover these greener approaches could sustainably utilize species during extract preparation and harnessing its nutraceutical and pharmaceutical potential. This study design could also be replicated on other valuable species or compounds for effective extraction of nutraceutical components and sustainable utilization of natural products.

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  • For MAE-assisted extractions in hydroethanolic systems, it is essential to evaluate the effect of pH, extraction time, and voltage of the equipment used during extraction.

    “For MAE-assisted extractions in hydroethanolic systems, it is essential to evaluate the effect of pH, extraction time, and voltage of the equipment used during extraction [36,37]”

    From Optimized microwave-assisted azadirachtin extraction using response surface methodology (2024) · cites this paper as [37]

    The Multiple-component analysis and MAE methods sections show that the study evaluated solvent pH, irradiation time, and microwave power. However, it used methanol rather than a hydroethanolic solvent and varied microwave power, not equipment voltage. The paper therefore supports evaluating related extraction conditions, but not the claim as stated. (LLM, conf=0.88)

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Cited by

Sorted by citations

Authors
Belwal, T., Pandey, A., Bhatt, I. D., & Rawal, R. S.
Affiliations
G.B. Pant Institute of Himalayan Environment and Development (IN)
Corresponding author
  • Belwal, Tarun
  • Pandey, Aseesh
  • Bhatt, Indra D.
ORCID
Venue
Scientific Reports
Publisher
Springer Science and Business Media LLC
Published
2020-01-22
Volume
10
Issue
1
Pages
917-917
ISSN
2045-2322 (ISSN-L)
DOI
10.1038/s41598-020-57585-8
PMC
PMC6976575
OpenAlex
W3013214374
Cited by
91
References
0
Citations by year
2026: 9 · 2025: 18 · 2024: 16 · 2023: 17 · 2022: 17 · 2021: 14
FWCI
7.19
Type
journal article
Language
en
Open access
Licence
Locations
  • Scientific Reports (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Funders
G.B. Pant National Institute of Himalayan Environment and Sustainable Development
Topics
  • Health Sciences › Medicine › Pharmacology › Berberine and alkaloids research
  • Health Sciences › Medicine › Complementary and alternative medicine › Traditional Chinese Medicine Analysis
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Agricultural and Biological Research
Keywords
microwave-assisted extraction, Berberis, berberine, palmatine, alkaloids, polyphenols, total phenolic content, antioxidant activity, extraction optimization, Berberis asiatica, nutraceuticals, green extraction
MeSH terms
Alkaloids, Antioxidants, Berberine, Dose-Response Relationship, Radiation, Microwaves, Radiation Dosage, Plant Roots, Berberis, Solid Phase Extraction, Polyphenols, Phytochemicals

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