Quantitative analysis of global protein stability rates in tissues
Open accessPublished version of a preprint
Abstract
Protein degradation is an essential mechanism for maintaining proteostasis in response to internal and external perturbations. Disruption of this process is implicated in many human diseases. We present a new technique, QUAD (Quantification of Azidohomoalanine Degradation), to analyze the global degradation rates in tissues using a non-canonical amino acid and mass spectrometry. QUAD analysis reveals that protein stability varied within tissues, but discernible trends in the data suggest that cellular environment is a major factor dictating stability. Within a tissue, different organelles and protein functions were enriched with different stability patterns. QUAD analysis demonstrated that protein stability is enhanced with age in the brain but not in the liver. Overall, QUAD allows the first global quantitation of protein stability rates in tissues, which will allow new insights and hypotheses in basic and translational research.
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References 81 · Cited by 19
- Authors
- McClatchy, D. B., Martínez-Bartolomé, S., Gao, Y., Lavallée-Adam, M., & Yates, J. R.
- Affiliations
- Scripps Research Institute (US)
- University of Illinois Chicago (US)
- Corresponding author
- Yates, John R.
- Venue
- Scientific Reports
- Published
- 2020-09-29
- Volume
- 10
- Issue
- 1
- Pages
- 15983-15983
- ISSN
- 2045-2322 (ISSN-L)
- OpenAlex
- W3091497311
- Cited by
- 19
- References
- 81
- Citations by year
- 2025: 5 · 2024: 2 · 2023: 6 · 2022: 3 · 2021: 3
- FWCI
- 0.97
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Scientific Reports (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (P41 GM103533)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ubiquitin and proteasome pathways
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
- Keywords
- protein stability, protein degradation, proteostasis, Quad, azidohomoalanine, brain aging, liver aging
- MeSH terms
- Proteostasis, Age Factors, Alanine, Animals, Brain, Liver, Male, Organ Specificity, Mass Spectrometry, Proteome, Mice, Protein Stability, Click Chemistry, Proteolysis
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