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High prevalence of multiple antibiotic resistance in clinical E. coli isolates from Bangladesh and prediction of molecular resistance determinants using WGS of an XDR isolate

Jain, Preeti, Bepari, Asim Kumar, Sen, Prosengit Kumer, Rafe, Tanzir, Imtiaz, Rashed, Hossain, Maqsud, Reza, Hasan Mahmud·2021·Scientific Reports
DOI 10.1038/s41598-021-02251-wPMC PMC8613203
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Abstract

Multi-drug-resistance (MDR) is a severe public health concern worldwide, and its containment is more challenging in developing countries due to poor antimicrobial resistance (AMR) surveillance and irrational use of antibiotics. The current study investigated 100 clinical E. coli isolates and revealed that 98% of them were MDR. PCR analysis using 25 selected isolates showed the predominance of metallo-β-lactamase gene bla NDM (80%) and ESBL genes bla OXA (48%) and bla CTX-M-15 (32%). The AmpC gene was detected in 68% of the isolates, while 32% was tet C positive. Notably, 34% of the isolates were resistant to carbapenem. Whole genome sequence (WGS) analysis of an extensively drug-resistant (XDR) isolate (L16) revealed the presence of the notorious sequence type 131 responsible for multi-drug-resistant infections, multiple antibiotic resistance genes (ARGs), virulence genes, and mobile genetic elements that pose risks to environmental transmission. Our results indicate that MDR is alarmingly increasing in Bangladesh that critically limits the treatment option against infections and contributes to further aggravation to the prevailing situation of MDR worldwide. The findings of this study will be valuable in designing sustainable strategies to contain MDR in the region.

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  • Multidrug-resistant bacteria have previously been associated with severe human illnesses like cholera, diarrhea, and other water-borne diseases in the Bangladeshi population.

    “Multidrug-resistant bacteria have previously been associated with severe human illnesses like cholera, diarrhea, and other water-borne diseases in the Bangladeshi population18,71–74.”

    From Impact of untreated tannery wastewater in the evolution of multidrug-resistant bacteria in Bangladesh (2024) · cites this paper as [73]

    The Introduction (page unavailable) identifies E. coli as a cause of enteric infections in Bangladesh and links its clinical threat to antibiotic resistance. The Discussion (page unavailable) reports multidrug-resistant E. coli in clinical settings and drinking water. The paper does not specifically associate resistant bacteria with cholera or establish that its clinical isolates caused the listed water-borne diseases. (LLM, conf=0.86)

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Metadata

Authors
Jain, P., Bepari, A. K., Sen, P. K., Rafe, T., Imtiaz, R., Hossain, M., & Reza, H. M.
Venue
Scientific Reports
Publisher
Springer Science and Business Media LLC
Published
2021-11-24
Volume
11
Issue
1
ISSN
2045-2322
DOI
10.1038/s41598-021-02251-w
PMC
PMC8613203
Cited by
69
References
1
Language
en
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