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Cited by 5DNA Repair MechanismsFungal and yeast genetics researchGenomics and Chromatin Dynamics

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Fission yeast Srr1 and Skb1 promote isochromosome formation at the centromere

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Mongia, Piyusha, Toyofuku, Naoko, Pan, Ziyi, Xu, Ran, Kinoshita, Yakumo, Oki, Keitaro, Takahashi, Hiroki, Ogura, Yoshitoshi, Hayashi, Tetsuya, Nakagawa, Takuro·2023·Communications Biology
DOI 10.1038/s42003-023-04925-9PMC PMC10219947

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Abstract

Rad51 maintains genome integrity, whereas Rad52 causes non-canonical homologous recombination leading to gross chromosomal rearrangements (GCRs). Here we find that fission yeast Srr1/Ber1 and Skb1/PRMT5 promote GCRs at centromeres. Genetic and physical analyses show that srr1 and skb1 mutations reduce isochromosome formation mediated by centromere inverted repeats. srr1 increases DNA damage sensitivity in rad51 cells but does not abolish checkpoint response, suggesting that Srr1 promotes Rad51-independent DNA repair. srr1 and rad52 additively, while skb1 and rad52 epistatically reduce GCRs. Unlike srr1 or rad52 , skb1 does not increase damage sensitivity. Skb1 regulates cell morphology and cell cycle with Slf1 and Pom1, respectively, but neither Slf1 nor Pom1 causes GCRs. Mutating conserved residues in the arginine methyltransferase domain of Skb1 greatly reduces GCRs. These results suggest that, through arginine methylation, Skb1 forms aberrant DNA structures leading to Rad52-dependent GCRs. This study has uncovered roles for Srr1 and Skb1 in GCRs at centromeres.

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Metadata

Authors
Mongia, P., Toyofuku, N., Pan, Z., Xu, R., Kinoshita, Y., Oki, K., Takahashi, H., Ogura, Y., Hayashi, T., & Nakagawa, T.
Affiliations
  • Forefront Research Center, Graduate School of Science, The University of Osaka (JP)
  • The University of Osaka (JP)
  • Chiba University (JP)
  • Kurume University (JP)
  • Kyushu University (JP)
Corresponding author
Nakagawa, Takuro
ORCID
Venue
Communications Biology
Publisher
Springer Science and Business Media LLC
Published
2023-05-26
Volume
6
Issue
1
Pages
551-551
ISSN
2399-3642 (ISSN-L)
DOI
10.1038/s42003-023-04925-9
PMC
PMC10219947
OpenAlex
W4378376520
Cited by
5
References
1
Citations by year
2026: 1 · 2025: 3 · 2023: 1
FWCI
0.44
Type
journal article
Language
en
Open access
Licence
Locations
  • Communications Biology (publishedVersion, cc-by)
  • Research Square (acceptedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Institutional Repositories DataBase (IRDB) (submittedVersion, cc-by)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • OUKA (Osaka University Knowledge Archive) (Osaka University) (submittedVersion)
Funders
  • MEXT | Japan Society for the Promotion of Science (JP26114711, 18K06060, 21H02402, JP23570212, 221S0002)
  • Uehara Memorial Foundation (202120462)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
Keywords
centromere, gross chromosomal rearrangements, RAD52, RAD51, arginine methylation, DNA damage sensitivity, DNA repair, genome stability, Schizosaccharomyces pombe
MeSH terms
Centromere, Humans, Methyltransferases, Protein-Arginine N-Methyltransferases, Protein Kinases, Schizosaccharomyces, RNA-Binding Proteins, Isochromosomes, Schizosaccharomyces pombe Proteins, Homologous Recombination

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