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Inducing the cell cycle arrest and apoptosis of oral KB carcinoma cells by hydroxychavicol: roles of glutathione and reactive oxygen species
Open accessAbstract
Hydroxychavicol (HC; 10 – 50 μ M), a betel leaf component, was found to suppress the 2% H 2 O 2‐induced lucigenin chemiluminescence for 53 – 75%. HC (0.02 – 2 μ M) was also able to trap superoxide radicals generated by a xanthine/xanthine oxidase system with 38 – 94% of inhibition. Hydroxyl radicals‐induced PUC18 plasmid DNA breaks was prevented by HC (1.6 – 16 μ M). A 24‐h exposure of KB cells to HC (0.5, 1 m M) resulted in 54 – 74% cell death as analysed by a 3‐(4,5‐dimethyl‐thiazol‐2‐yl)‐2,5‐diphenyl‐tetrazolium bromide (MTT) assay. HC (10, 50 μ M) further suppressed the growth of KB cells (15 and 76%, respectively). Long‐term colony formation of KB cells was inhibited by 51% with 10 μ M HC. Pretreatment of KB cells with 100 μ M HC inhibited the attachment of KB cells to type I collagen and fibronectin by 59 and 29%, respectively. Exposure of KB cells to 0.1 m M HC for 24 h resulted in cell cycle arrest at late S and G2/M phase. Increasing the HC concentration to 0.25 and 0.5 m M led to apoptosis as revealed by detection of sub‐G 0/G 1 peaks with a concomitant decrease in the number of cells residing in late S and G 2/M phase. Inducing the apoptosis of KB cells by HC was accompanied by marked depletion in reduced form of GSH (>0.2 m M) and the increasing of reactive oxygen species production (>0.1 m M) as analysed by CMF‐ and DCF‐single cell fluorescence flow cytometry. These results indicate that HC exerts antioxidant property at low concentration. HC also inhibits the growth, adhesion and cell cycle progression of KB cells, whereas its induction of KB cell apoptosis (HC>0.1 m M) was accompanied by cellular redox changes. British Journal of Pharmacology (2002) 135, 619–630; doi:10.1038/sj.bjp.0704492
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“Hydroxychavicol is the major phenolic component, isolated from the aqueous extract of P. betle L., leaf has been reported to possess antinitrosation, antimutagenic, anticarcinogenic activities [10].”
From In vitro antifungal activity of hydroxychavicol isolated from Piper betle L (2010) · cites this paper as [10]
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Betel quid chewing, cigarette smoking and alcohol consumption related to oral cancer in Taiwan
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Rapid Communication: Oxidative Stress Induces Apoptosis in Embryonic Cortical Neurons
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In vitro antifungal activity of hydroxychavicol isolated from Piper betle L
Ali, I., Khan, F. G., Suri, K. A., Gupta, B. D., Satti, N. K., Dutt, P., Afrin, F., Qazi, G. N., & Khan, I. A. (2010) · Annals of Clinical Microbiology and Antimicrobials · doi:10.1186/1476-0711-9-7 · Cited by 176
Sharma, S., Khan, I. A., Ali, I., Ali, F., Kumar, M., Kumar, A., Johri, R. K., Abdullah, S. T., Bani, S., Pandey, A., et al. (2009) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.00045-08 · Cited by 131
Chang, M. C., Uang, B. J., Tsai, C. Y., Wu, H. L., Lin, B. R., Lee, C. S., Chen, Y. J., Chang, C. H., Tsai, Y. L., Kao, C. J., et al. (2007) · British Journal of Pharmacology · doi:10.1038/sj.bjp.0707367 · Cited by 58
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Metadata
- Authors
- Chang, M. C., Uang, B. J., Wu, H. L., Lee, J. J., Hahn, L. J., & Jeng, J. H.
- Affiliations
- Chang Gung University (TW)
- National Tsing Hua University (TW)
- National Taiwan University (TW)
- Corresponding author
- Jeng, J H
- Venue
- British Journal of Pharmacology
- Publisher
- Wiley
- Published
- 2002-02
- Volume
- 135
- Issue
- 3
- Pages
- 619-630
- ISSN
- 0007-1188 (ISSN-L)
- 1476-5381
- PMC
- PMC1573166
- OpenAlex
- W2038661189
- Cited by
- 99
- References
- 73
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 4 · 2023: 10 · 2022: 3 · 2021: 4 · 2020: 1 · 2019: 2 · 2018: 4 · 2017: 2 · 2016: 2 · 2015: 1 · 2014: 7 · 2013: 4 · 2012: 9
- FWCI
- 5.33
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- British Journal of Pharmacology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Toxicology › Bioactive Compounds and Antitumor Agents
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cancer and biochemical research
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Medicinal Plant Research
- Keywords
- hydroxychavicol, apoptosis, cell cycle arrest, glutathione, reactive oxygen species, antioxidant activity, superoxide radical scavenging, cell adhesion, G2/M cell cycle arrest
- MeSH terms
- Antineoplastic Agents, Areca, Cell Adhesion, Cell Cycle, Collagen, Dose-Response Relationship, Drug, Eugenol, Fibronectins, Glutathione, Growth Inhibitors, Humans, KB Cells, Anticarcinogenic Agents, Apoptosis, Reactive Oxygen Species, Oxidative Stress, Cell Size
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- Chang, M. C., Uang, B. J., Wu, H. L., Lee, J. J., Hahn, L. J., & Jeng, J. H. (2002). Inducing the cell cycle arrest and apoptosis of oral KB carcinoma cells by hydroxychavicol: roles of glutathione and reactive oxygen species. British Journal of Pharmacology. https://doi.org/10.1038/sj.bjp.0704492
