A noninvasive oral rinse assay to monitor engraftment, neutrophil tissue delivery and susceptibility to infection following HSCT in pediatric patients
Abstract
The time interval between neutrophil tissue delivery and blood confirmed engraftment following hematopoietic stem cell transplantation (HSCT) may serve as an indicator of patient susceptibility to infection. Using an oral rinse protocol, we studied neutrophil tissue delivery kinetics and its relationship to clinical parameters post-HSCT in 29 pediatric patients. Oral neutrophil counts were compared to circulating neutrophil levels, oral mucositis scores and infection-related febrile episodes after engraftment. Blood engraftment (BE) is currently defined by a blood neutrophil count of > or =0.5 x 10(9)/l. We defined oral engraftment (OE) as the day neutrophils returned in the mouth post-HSCT (> or =0.25 x 10(4)/ml oral neutrophils in the rinse sample). We found that neutrophils reappeared 6.3+/-3.9 s.d. days earlier in the mouth than in the circulation enabling us to identify successful engraftment almost 1 week sooner than using blood count values alone. Furthermore, the time-span between OE and BE was inversely related to the number of infection-related febrile episodes post-BE. We conclude that monitoring the timing of neutrophil tissue delivery through a rapid oral rinse may yield important insights into the biology of neutrophil recovery during and after engraftment and the factors associated with neutrophil tissue recruitment.
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Research suggests that the oral cavity may provide an early opportunity to non-invasively monitor the innate immune system.
“Research suggests that the oral cavity may provide an early opportunity to non-invasively monitor the innate immune system [13-15].”
From Oral neutrophils are an independent marker of the systemic inflammatory response after cardiac bypass (2014) · cites this paper as [15]
The recruitment of neutrophils by the oral biofilm may serve as a non-invasive measure of in vivo systemic neutrophil activation and chemotaxis.
“The recruitment of neutrophils by the oral biofilm may serve as a non-invasive measure of in vivo systemic neutrophil activation and chemotaxis [13-15].”
From Oral neutrophils are an independent marker of the systemic inflammatory response after cardiac bypass (2014) · cites this paper as [15]
The timing of neutrophil recovery to the oral cavity following HSCT has predictive value for future infections post-engraftment in a paediatric population and is predictive of prognosis in an adult HSCT population.
“The timing of neutrophil recovery to the oral cavity following HSCT was recently found to have predictive value for future infections post-engraftment in a paediatric population [15] and be predictive of prognosis in an adult HSCT population [34].”
From Oral neutrophils are an independent marker of the systemic inflammatory response after cardiac bypass (2014) · cites this paper as [15]
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References 11 · Cited by 34
- Authors
- Cheretakis, C., Dror, Y., & Glogauer, M.
- Affiliations
- University of Toronto (CA)
- Hospital for Sick Children (CA)
- Corresponding author
- Glogauer, M
- ORCID
- Venue
- Bone Marrow Transplantation
- Published
- 2005-06-06
- Volume
- 36
- Issue
- 3
- Pages
- 227-232
- ISSN
- 0268-3369 (ISSN-L)
- 1476-5365
- OpenAlex
- W2075826806
- Cited by
- 34
- References
- 11
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 1 · 2023: 2 · 2020: 4 · 2019: 2 · 2017: 1 · 2016: 2 · 2015: 1 · 2014: 2 · 2013: 1 · 2012: 3
- FWCI
- 1.18
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Bone Marrow Transplantation (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Hospital for Sick Children
- Canadian Institutes of Health Research
- Topics
- Health Sciences › Medicine › Hematology › Hematopoietic Stem Cell Transplantation
- Health Sciences › Medicine › Oncology › Neutropenia and Cancer Infections
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Oral health in cancer treatment
- Keywords
- hematopoietic stem cell transplantation, neutrophil recovery, infection susceptibility, febrile neutropenia, oral mucositis, pediatric patients
- MeSH terms
- Adolescent, Adult, Child, Disease Susceptibility, Female, Humans, Infections, Kinetics, Leukemia, Leukocyte Count, Male, Microscopy, Fluorescence, Mouth Mucosa, Mouthwashes, Neutrophils, Time Factors, Hematopoietic Stem Cell Transplantation
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