The chromatin remodelling complex WSTF–SNF2h interacts with nuclear myosin 1 and has a role in RNA polymerase I transcription
Open accessAbstract
Nuclear actin and myosin 1 (NM1) are key regulators of gene transcription. Here, we show by biochemical fractionation of nuclear extracts, protein-protein interaction studies and chromatin immunoprecipitation assays that NM1 is part of a multiprotein complex that contains WICH, a chromatin remodelling complex containing WSTF (Williams syndrome transcription factor) and SNF2h. NM1, WSTF and SNF2h were found to be associated with RNA polymerase I (Pol I) and ribosomal RNA genes (rDNA). RNA interference-mediated knockdown of NM1 and WSTF reduced pre-rRNA synthesis in vivo, and antibodies to WSTF inhibited Pol I transcription on pre-assembled chromatin templates but not on naked DNA. The results indicate that NM1 cooperates with WICH to facilitate transcription on chromatin.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Baz1b was chosen as the best candidate for MommeD10 because it contains a bromodomain and has been shown to form chromatin remodeling complexes and associate with replication foci and promoters.
“The linked interval for MommeD10 contains 52 genes and Baz1b was chosen as the best candidate because it contains a bromodomain (a domain commonly associated with chromatin remodelers) and has recently been shown to form at least two chromatin remodeling complexes and associate with replication foci and promoters [23-25].”
From A genome-wide screen for modifiers of transgene variegation identifies genes with critical roles in development (2008) · cites this paper as [25]
Both MommeD5 and MommeD10 genes encode proteins involved in histone modification and chromatin remodeling.
“This is consistent with the fact that both genes encode proteins involved in histone modification and chromatin remodeling [21,23-25,30-33].”
From A genome-wide screen for modifiers of transgene variegation identifies genes with critical roles in development (2008) · cites this paper as [25]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
The citation lists haven’t been fetched yet
References 29 · Cited by 171
- Authors
- Percipalle, P., Fomproix, N., Cavellán, E., Voit, R., Reimer, G., Krüger, T., Thyberg, J., Scheer, U., Grummt, I., & Östlund Farrants, A.
- Affiliations
- Karolinska Institutet (SE)
- Stockholm University (SE)
- German Cancer Research Center (DE)
- University of Würzburg (DE)
- Corresponding author
- Percipalle, Piergiorgio
- Venue
- The EMBO Reports
- Published
- 2006-03-03
- Volume
- 7
- Issue
- 5
- Pages
- 525-530
- ISSN
- 1469-221X (ISSN-L)
- 1469-3178
- PMC
- PMC1479564
- OpenAlex
- W2039133783
- Cited by
- 171
- References
- 29
- Citations by year
- 2026: 3 · 2025: 2 · 2024: 6 · 2023: 1 · 2022: 7 · 2021: 5 · 2020: 9 · 2019: 4 · 2018: 8 · 2017: 7 · 2016: 18 · 2015: 8 · 2014: 6 · 2013: 9 · 2012: 11
- FWCI
- 5.43
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- EMBO Reports (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Deutsche Forschungsgemeinschaft
- Vetenskapsrådet
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Chromatin Remodeling and Cancer
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
- Keywords
- WSTF, SNF2H, chromatin remodeling, RNA polymerase I transcription, ribosomal DNA, pre-rRNA synthesis
- MeSH terms
- Bromodomain Containing Proteins, Adenosine Triphosphatases, Cell Nucleus, Chromosomal Proteins, Non-Histone, HeLa Cells, Humans, Nuclear Proteins, Protein Binding, RNA Polymerase I, Transcription Factors, Transcription, Genetic, Myosin Type I, Chromatin Assembly and Disassembly, Intracellular Signaling Peptides and Proteins
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
