CoChat
Sign Up
Cited by 22Trypanosoma species research and implicationsResearch on Leishmaniasis StudiesInvertebrate Immune Response Mechanisms

Gene expression profiling in a mouse model for African trypanosomiasis

Open access
Kierstein, S, Noyes, H, Naessens, J, Nakamura, Y, Pritchard, C, Gibson, J, Kemp, S, Brass, A·2006·Genes & Immunity
DOI 10.1038/sj.gene.6364345PMC PMC1991335
Open this paper in CoChat — free account

Abstract

This study aimed to provide the foundation for an integrative approach to the identification of the mechanisms underlying the response to infection with Trypanosoma congolense, and to identify pathways that have previously been overlooked. We undertook a large-scale gene expression analysis study comparing susceptible A/J and more tolerant C57BL/6 mice. In an initial time course experiment, we monitored the development of parasitaemia and anaemia in every individual. Based on the kinetics of disease progression, we extracted total RNA from liver at days 0, 4, 7, 10 and 17 post infection and performed a microarray analysis. We identified 64 genes that were differentially expressed in the two strains in non-infected animals, of which nine genes remained largely unaffected by the disease. Gene expression profiling at stages of low, peak, clearance and recurrence of parasitaemia suggest that susceptibility is associated with high expression of genes coding for chemokines (e.g. Ccl24, Ccl27 and Cxcl13), complement components (C1q and C3) and interferon receptor alpha (Ifnar1). Additionally, susceptible A/J mice expressed higher levels of some potassium channel genes. In contrast, messenger RNA levels of a few immune response, metabolism and protease genes (e.g. Prss7 and Mmp13) were higher in the tolerant C57BL/6 strain as compared to A/J.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1038/sj.gene.6364345

Cites

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

No references could be resolved yet.

Research works printed but not linked: 38

  1. [1]Torr SJ Hargrove JW Vale GA Towards a rational policy for dealing with tsetse Trends Parasitol 2005 21 537 541 16140579
  2. [2]Mansfield JM Paulnock DM Regulation of innate and acquired immunity in African trypanosomiasis Parasite Immunol 2005 27 361 371 16179030
  3. [3]Omamo SW d’Ieteren GD Managing animal trypanosomosis in Africa: issues and options Rev Sci Tech 2003 22 989 1002 15005555
  4. [4]Nyame AK Kawar ZS Cummings RD Antigenic glycans in parasitic infections: implications for vaccines and diagnostics Arch Biochem Biophys 2004 426 182 200 15158669
  5. [5]Hill EW O’Gorman GM Agaba M Gibson JP Hanotte O Kemp SJ Understanding bovine trypanosomiasis and trypanotolerance: the promise of functional genomics Vet Immunol Immunopathol 2005 105 247 258 15808304
  6. [6]Maichomo MW Ndungu JM Ngare PM Ole-Mapenay IM The performance of Orma Boran and Maasai Zebu cross-breeds in a trypanosomosis endemic area of Nguruman, south western Kenya Onderstepoort J Vet Res 2005 72 87 93 15991707
  7. [7]Williams DJ Taylor K Newson J Gichuki B Naessens J The role of anti-variable surface glycoprotein antibody responses in bovine trypanotolerance Parasite Immunol 1996 18 209 218 9223176
  8. [8]Clapcott SJ Teale AJ Kemp SJ Evidence for genomic imprinting of the major QTL controlling susceptibility to trypanosomiasis in mice Parasite Immunol 2000 22 259 263 10792765
  9. [9]Iraqi F Clapcott SJ Kumari P Haley CS Kemp SJ Teale AJ Fine mapping of trypanosomiasis resistance loci in murine advanced intercross lines Mamm Genome 2000 11 645 648 10920233
  10. [10]Baetselier PD Namangala B Noel W Brys L Pays E Beschin A Alternative versus classical macrophage activation during experimental African trypanosomosis Int J Parasitol 2001 31 575 587 11334945
  11. [11]Buza J Sileghem M Gwakisa P Naessens J CD5+ B lymphocytes are the main source of antibodies reactive with non-parasite antigens in Trypanosoma congolense-infected cattle Immunology 1997 92 226 233 9415031 PMC1364063
  12. [12]Duleu S Vincendeau P Courtois P Semballa S Lagroye I Daulouede S Mouse strain susceptibility to trypanosome infection: an arginase-dependent effect J Immunol 2004 172 6298 6303 15128819
  13. [13]Hertz CJ Mansfield JM IFN-gamma-dependent nitric oxide production is not linked to resistance in experimental African trypanosomiasis Cell Immunol 1999 192 24 32 10066343
  14. [14]Nakamura Y Naessens J Takata M Taniguchi T Sekikawa K Gibson J Susceptibility of heat shock protein 70.1-deficient C57BL/6 J, wild-type C57BL/6 J and A/J mice to Trypanosoma congolense infection Parasitol Res 2003 90 171 174 12756556
  15. [15]Otesile EB Lee M Tabel H Plasma levels of proteins of the alternative complement pathway in inbred mice that differ in resistance to Trypanosoma congolense infections J Parasitol 1991 77 958 964 1838113
  16. [16]Tabel H Kaushik RS Uzonna J Experimental African trypanosomiasis: differences in cytokine and nitric oxide production by macrophages from resistant and susceptible mice Pathobiology 1999 67 273 276 10725801
  17. [17]Uzonna JE Kaushik RS Gordon JR Tabel H Immunoregulation in experimental murine Trypanosoma congolense infection: anti-IL-10 antibodies reverse trypanosome-mediated suppression of lymphocyte proliferation in vitro and moderately prolong the lifespan of genetically susceptible BALB/c mice Parasite Immunol 1998 20 293 302 9651931
  18. [18]Uzonna JE Kaushik RS Gordon JR Tabel H Cytokines and antibody responses during Trypanosoma congolense infections in two inbred mouse strains that differ in resistance Parasite Immunol 1999 21 57 71 10101716
  19. [19]Miller CG Justus DE Jayaraman S Kotwal GJ Severe and prolonged inflammatory response to localized cowpox virus infection in footpads of C5-deficient mice: investigation of the role of host complement in poxvirus pathogenesis Cell Immunol 1995 162 326 332 7743560
  20. [20]Ooi YM Colten HR Genetic defect in secretion of complement C5 in mice Nature 1979 282 207 208 492335
  21. [21]Joseph SB Bradley MN Castrillo A Bruhn KW Mak PA Pei L LXR-dependent gene expression is important for macrophage survival and the innate immune response Cell 2004 119 299 309 15479645
  22. [22]Kay JG Murray RZ Pagan JK Stow JL Cytokine secretion via cholesterol-rich lipid raft-associated SNAREs at the phagocytic cup J Biol Chem 2006 281 11949 11954 16513632
  23. [23]Manley PN Ancsin JB Kisilevsky R Rapid recycling of cholesterol: the joint biologic role of C-reactive protein and serum amyloid A Med Hypotheses 2006 66 784 792 16337748
  24. [24]Hancock JF Lipid rafts: contentious only from simplistic standpoints Nat Rev Mol Cell Biol 2006 7 456 462 16625153 PMC2782566
  25. [25]Machleder D Ivandic B Welch C Castellani L Reue K Lusis AJ Complex genetic control of HDL levels in mice in response to an atherogenic diet. Coordinate regulation of HDL levels and bile acid metabolism J Clin Invest 1997 99 1406 1419 9077551 PMC507957
  26. [26]Schwarz M Davis DL Vick BR Russell DW Genetic analysis of intestinal cholesterol absorption in inbred mice J Lipid Res 2001 42 1801 1811 11714849
  27. [27]Suto J Takahashi Y Sekikawa K Quantitative trait locus analysis of plasma cholesterol and triglyceride levels in C57BL/6J × RR F2 mice Biochem Genet 2004 42 347 363 15524312
  28. [28]Wang X Le Roy I Nicodeme E Li R Wagner R Petros C Using advanced intercross lines for high-resolution mapping of HDL cholesterol quantitative trait loci Genome Res 2003 13 1654 1664 12805272 PMC403739
  29. [29]Wittenburg H Lyons MA Li R Kurtz U Wang X Mossner J QTL mapping for genetic determinants of lipoprotein cholesterol levels in independent and combined crosses of inbred mouse strains J Lipid Res 2006 47 1780 1790 16685081
  30. [30]Naessens J Kitani H Nakamura Y Yagi Y Sekikawa K Iraqi F TNF-alpha mediates the development of anaemia in a murine Trypanosoma brucei rhodesiense infection, but not the anaemia associated with a murine Trypanosoma congolense infection Clin Exp Immunol 2005 139 405 410 15730385 PMC1809320
  31. [31]Authie E Pobel T Serum haemolytic complement activity and C3 levels in bovine trypanosomosis under natural conditions of challenge - early indications of individual susceptibility to disease Vet Parasitol 1990 35 43 59 2343531
  32. [32]Shi M Pan W Tabel H Experimental African trypanosomiasis: IFN-gamma mediates early mortality Eur J Immunol 2003 33 108 118 12594839
  33. [33]Lakhdar-Ghazal F Blonski C Willson M Michels P Perie J Glycolysis and proteases as targets for the design of new anti-trypanosome drugs Curr Top Med Chem 2002 2 439 456 11966466
  34. [34]Nantulya VM Musoke AJ Rurangirwa FR Barbet AF Ngaira JM Katende JM Immune depression in African trypanosomiasis: the role of antigenic competition Clin Exp Immunol 1982 47 234 242 7075022 PMC1536523
  35. [35]Lanham SM Separation of trypanosomes from the blood of infected rats and mice by anion-exchangers Nature 1968 218 1273 1274 5656665
  36. [36]Chomczynski P Sacchi N Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction Anal Biochem 1987 162 156 159 2440339
  37. [37]Hancock D Wilson M Velarde G Morrison N Hayes A Hulme H maxdLoad2 and maxdBrowse: standards-compliant tools for microarray experimental annotation, data management and dissemination BMC Bioinformatics 2005 6 264 16269077 PMC1298287
  38. [38]Fang Y Brass A Hoyle DC Hayes A Bashein A Oliver SG A model-based analysis of microarray experimental error and normalisation Nucleic Acids Res 2003 31 e96 12907748 PMC169988

Cited by

Sorted by citations

Authors
Kierstein, S., Noyes, H., Naessens, J., Nakamura, Y., Pritchard, C., Gibson, J., Kemp, S., & Brass, A.
Affiliations
  • International Livestock Research Institute (KE)
  • University of Liverpool (GB)
  • Japan International Research Center for Agricultural Sciences (JP)
  • Mary Lyon Centre at MRC Harwell (GB)
  • University of Manchester (GB)
Corresponding author
Kierstein, S
ORCID
Venue
Genes & Immunity
Publisher
Springer Science and Business Media LLC
Published
2006-10-26
Volume
7
Issue
8
Pages
667-679
ISSN
  • 1466-4879 (ISSN-L)
  • 1476-5470
DOI
10.1038/sj.gene.6364345
PMC
PMC1991335
OpenAlex
W2100517828
Cited by
22
References
0
Citations by year
2023: 1 · 2022: 1 · 2021: 1 · 2020: 1 · 2019: 2 · 2018: 2 · 2014: 1 · 2013: 1
FWCI
2.21
Type
journal article
Language
en
Open access
Licence
Locations
  • Genes and Immunity (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
  • EPubDev Repository (submittedVersion)
  • CGSPace A Repository of Agricultural Research Outputs (Consultative Group for International Agricultural Research) (submittedVersion)
  • Research Explorer (The University of Manchester) (submittedVersion)
  • RUNE (Research UNE) (submittedVersion)
Funders
  • Wellcome Trust (066764)
  • Deutsche Forschungsgemeinschaft
  • Medical Research Council
Topics
  • Health Sciences › Medicine › Epidemiology › Trypanosoma species research and implications
  • Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Research on Leishmaniasis Studies
  • Life Sciences › Immunology and Microbiology › Immunology › Invertebrate Immune Response Mechanisms
Keywords
sleeping sickness, Trypanosoma congolense, gene expression profiling, host susceptibility, A/J mice, C57BL/6 mice, parasitemia, anemia, chemokines, complement system, potassium channels, liver transcriptome, host-parasite interactions
MeSH terms
Animals, Gene Expression Regulation, Liver, Mice, Inbred C57BL, Time Factors, Trypanosoma congolense, Trypanosomiasis, African, Cluster Analysis, Reverse Transcriptase Polymerase Chain Reaction, Gene Expression Profiling, Microarray Analysis, Mice

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review