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Constitutive phosphorylation of Akt/PKB protein in acute myeloid leukemia: its significance as a prognostic variable

Min, Y H, Eom, J I, Cheong, J W, Maeng, H O, Kim, J Y, Jeung, H K, Lee, S T, Lee, M H, Hahn, J S, Ko, Y W·2003·Leukemia
DOI 10.1038/sj.leu.2402874
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Figure 1 Western blot analysis demonstrated the constitutive phosphorylation of Ser473 and Thr308 residues in the total Akt/PKB-positive AML cases (lanes 3-10), but not in the CD34-positive normal bone marrow cells (lanes 1-2) or the total Akt/PKB-negative AML cases (lane 11-12). HL60 cell lines were loaded to provide a standard curve.
Figure 1 Western blot analysis demonstrated the constitutive phosphorylation of Ser473 and Thr308 residues in the total Akt/PKB-positive AML cases (lanes 3-10), but not in the CD34-positive normal bone marrow cells (lanes 1-2) or the total Akt/PKB-negative AML cases (lane 11-12). HL60 cell lines were loaded to provide a standard curve.CC-BY-NC-ND · From the PDF
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Figure 2 PI-3K-dependent Akt/PKB phosphorylation in AML cells. (a) Both pSer473-positive and negative cells were immunoprecipitated with the antibody against p85 subunit of PI-3K. (b) Blocking the PI-3K, by culturing AML cells for 30 min in the presence of LY294002, significantly abrogated the phosphorylation of Ser473 and Thr308 in AML cells.
Figure 2 PI-3K-dependent Akt/PKB phosphorylation in AML cells. (a) Both pSer473-positive and negative cells were immunoprecipitated with the antibody against p85 subunit of PI-3K. (b) Blocking the PI-3K, by culturing AML cells for 30 min in the presence of LY294002, significantly abrogated the phosphorylation of Ser473 and Thr308 in AML cells.CC-BY-NC-ND · From the PDF
Figure 3 (a) Kaplan-Meier overall survival curve by the constitutive expression of total Akt/PKB expression in AML patients. (b) Kaplan-Meier overall survival curve by constitutive expression of pSer473 in AML patients. Statistical analysis performed by the log-rank test, corresponding P values indicated in the figure.
Figure 3 (a) Kaplan-Meier overall survival curve by the constitutive expression of total Akt/PKB expression in AML patients. (b) Kaplan-Meier overall survival curve by constitutive expression of pSer473 in AML patients. Statistical analysis performed by the log-rank test, corresponding P values indicated in the figure.CC-BY-NC-ND · From the PDF

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Research works printed but not linked: 8

  1. Jordan CT . Unique molecular and cellular features of acute myelogenous leukemia stem cells. Leukemia 2002; 16: 559–562. · DOI
  2. Tse KF, Mukherjee G, Small D . Constitutive activation of FLT3 stimulates multiple intracellular signal transducers and results in transformation. Leukemia 2000; 14: 1766–1776. · DOI
  3. Lee JT Jr, McCubrey JA . The Raf/MEK/ERK signal transduction cascade as a target for chemotherapeutic intervention in leukemia. Leukemia 2002; 16: 486–507. · DOI
  4. Alessi DR, James SR, Downes CP, Holmes AB, Gaffney PR, Reese CB et al. Characterization of a 3-phosphoinositide-dependent protein kinase which phosphorylates and activates protein kinase Bα. Curr Biol 1997; 7: 261–269. · DOI
  5. Hsu J, Shi Y, Krajewski S, Renner S, Fisher M, Reed JC et al. The Akt kinase is activated in multiple myeloma tumor cells. Blood 2001; 98: 2853–2855. · DOI
  6. Nicholson KM, Anderson NG . The protein kinase B/Akt signall-ing pathway in human malignancy. Cell Signal 2002; 14: 381–395. · DOI
  7. Brognard J, Clark AS, Ni Y, Dennis PA . Akt/protein kinase B is constitutively active in non-small cell lung cancer cells and promotes cellular survival and resistance to chemotherapy and radiation. Cancer Res 2001; 61: 3986–3997.
  8. Nakatani K, Thompson DA, Barthel A, Sakaue H, Liu W, Weigel RJ et al. Up-regulation of Akt3 in estrogen receptor-deficient breast cancers and androgen-independent prostate cancer lines. J Biol Chem 1999; 274: 21528–21532. · DOI

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Metadata

Authors
Min, Y. H., Eom, J. I., Cheong, J. W., Maeng, H. O., Kim, J. Y., Jeung, H. K., Lee, S. T., Lee, M. H., Hahn, J. S., & Ko, Y. W.
Venue
Leukemia
Publisher
Springer Science and Business Media LLC
Published
2003-05-01
Volume
17
Issue
5
Pages
995-997
ISSN
  • 0887-6924
  • 1476-5551
DOI
10.1038/sj.leu.2402874
Cited by
192
References
0
Language
en
Licence

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