Identification of radiation-specific responses from gene expression profile
Open accessAbstract
The responses to ionizing radiation (IR) in tumors are dependent on cellular context. We investigated radiation-related expression patterns in Jurkat T cells with nonsense mutation in p53 using cDNA microarray. Expression of 2400 genes in gamma-irradiated cells was distinct from other stimulations like anti-CD3, phetohemagglutinin (PHA) and concanavalin A (ConA) in unsupervised clustering analysis. Among them, 384 genes were selected for their IR-specific changes to make 'RadChip'. In spite of p53 status, every type of cells showed similar patterns in expression of these genes upon gamma-radiation. Moreover, radiation-induced responses were clearly separated from the responses to other genotoxic stress like UV radiation, cisplatin and doxorubicin. We focused on two IR-related genes, phospholipase Cgamma2 (PLCG2) and cytosolic epoxide hydrolase (EPHX2), which were increased at 12 h after gamma-radiation in RT-PCR. TPCK could suppress the induction of these two genes in either of Jurkat T cells and PBMCs, which might suggest the transcriptional regulation of PLCG2 and EPHX2 by NF-kappaB upon gamma-radiation. From these results, we could identify the IR-specific genes from expression profiling, which can be used as radiation biomarkers to screen radiation exposure as well as probing the mechanism of cellular responses to ionizing radiation.
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PLCγ2 was noted as a potential biomarker of radiation exposure through its upregulation after ionizing radiation.
“PLCγ2 as was also noted as a potential biomarker of radiation exposure through its upregulation after ionizing radiation (158).”
From The role of PLCγ2 in immunological disorders, cancer, and neurodegeneration (2021) · cites this paper as [158]
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The role of PLCγ2 in immunological disorders, cancer, and neurodegeneration
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- Authors
- Park, W.-Y., Hwang, C.-I., Im, C.-N., Kang, M.-J., Woo, J.-H., Kim, J.-H., Kim, Y. S., Kim, J.-H., Kim, H., Kim, K.-A., Yu, H.-J., Lee, S.-J., Lee, Y.-S., & Seo, J.-S.
- Affiliations
- Seoul National University (KR)
- Macrogen (South Korea) (KR)
- Corresponding author
- Seo, Jeong-Sun
- ORCID
- Venue
- Oncogene
- Published
- 2002-12-04
- Volume
- 21
- Issue
- 55
- Pages
- 8521-8528
- ISSN
- 0950-9232 (ISSN-L)
- 1476-5594
- OpenAlex
- W2167004083
- Cited by
- 113
- References
- 0
- Citations by year
- 2026: 1 · 2024: 1 · 2022: 2 · 2021: 3 · 2020: 2 · 2018: 2 · 2017: 1 · 2016: 6 · 2015: 8 · 2014: 2 · 2013: 4 · 2012: 2
- FWCI
- 8.28
- Type
- journal article
- Language
- en
- Open access
- Licence
- Locations
- Oncogene (publishedVersion)
- PubMed (publishedVersion)
- YUHSpace (Yonsei University Medical Library) (submittedVersion, cc-by-nc-nd)
- Topics
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Effects of Radiation Exposure
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gene expression and cancer classification
- Keywords
- ionizing radiation response, gene expression profiling, gamma irradiation, Jurkat cells, PLCγ2, EPHX2, NF-κB, TPCK, radiation biomarkers, genotoxic stress, T cell activation
- MeSH terms
- Cell Division, Concanavalin A, Humans, Phytohemagglutinins, Radiation, Ionizing, T-Lymphocytes, Signal Transduction, Gene Expression Regulation, Neoplastic, Apoptosis, CD3 Complex, Jurkat Cells, Oligonucleotide Array Sequence Analysis, Gene Expression Profiling
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