Impact of intestinal microbiota on intestinal luminal metabolome
Open accessAbstract
Low-molecular-weight metabolites produced by intestinal microbiota play a direct role in health and disease. In this study, we analyzed the colonic luminal metabolome using capillary electrophoresis mass spectrometry with time-of-flight (CE-TOFMS) -a novel technique for analyzing and differentially displaying metabolic profiles- in order to clarify the metabolite profiles in the intestinal lumen. CE-TOFMS identified 179 metabolites from the colonic luminal metabolome and 48 metabolites were present in significantly higher concentrations and/or incidence in the germ-free (GF) mice than in the Ex-GF mice (p < 0.05), 77 metabolites were present in significantly lower concentrations and/or incidence in the GF mice than in the Ex-GF mice (p < 0.05), and 56 metabolites showed no differences in the concentration or incidence between GF and Ex-GF mice. These indicate that intestinal microbiota highly influenced the colonic luminal metabolome and a comprehensive understanding of intestinal luminal metabolome is critical for clarifying host-intestinal bacterial interactions.
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The microbiome metabolizes substantial quantities of dietary arginine and may regulate arginine availability to the immune system to some extent.
“This indicates that the microbiome metabolizes substantial quantities of dietary arginine and may thus regulate the availability of arginine to the immune system to a certain extent (Mardinoglu et al., 2015; Matsumoto et al., 2012).”
From Understanding the Holobiont: How Microbial Metabolites Affect Human Health and Shape the Immune System (2017) · cites this paper as Matsumoto et al., 2012
Germ-free mice retain significantly higher amounts of arginine in their intestines than mice colonized by gut microbes.
“Germ-free mice retain significantly higher amounts of arginine in their intestines than mice colonized by gut microbes.”
From Understanding the Holobiont: How Microbial Metabolites Affect Human Health and Shape the Immune System (2017) · cites this paper as Matsumoto et al., 2012
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References 49 · Cited by 334
Research works printed but not linked: 2
- Mitsuoka T. & Hayakawa K. Die Faekalflora bei Menschen. I. Mitteilung: Die Zusammensetzung der Faekalflora der verschiedenen Altersgruppen. Zbl. Bakt. Hyg. I Abt. Orig. A 223, 333– 342 (1972). 4145840
- Matsumoto M., Sakamoto M. & Benno Y. Dynamics of fecal microbiota in hospitalized elderly fed probiotic LKM512 yogurt. Microbiol Immunol 53, 421– 432 (2009). 19659926
- Authors
- Matsumoto, M., Kibe, R., Ooga, T., Aiba, Y., Kurihara, S., Sawaki, E., Koga, Y., & Benno, Y.
- Affiliations
- RIKEN (JP)
- Human Metabolome Technologies, Inc. (Japan) (JP)
- Tokai University (JP)
- Kyoto University (JP)
- Corresponding author
- Matsumoto, Mitsuharu
- Venue
- Scientific Reports
- Published
- 2012-01-25
- Volume
- 2
- Issue
- 1
- Pages
- 233-233
- ISSN
- 2045-2322 (ISSN-L)
- PMC
- PMC3380406
- OpenAlex
- W1973217994
- Cited by
- 334
- References
- 49
- Citations by year
- 2026: 10 · 2025: 24 · 2024: 25 · 2023: 23 · 2022: 29 · 2021: 27 · 2020: 31 · 2019: 34 · 2018: 36 · 2017: 22 · 2016: 22 · 2015: 13 · 2014: 24 · 2013: 10 · 2012: 4
- FWCI
- 6.55
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Scientific Reports (publishedVersion, cc-by-nc-sa)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-sa)
- PubMed Central (publishedVersion, cc-by-nc-sa)
- PubMed (publishedVersion)
- Funders
- Bio-oriented Technology Research Advancement Institution
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
- Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
- Keywords
- gut microbiota, germ-free mice, host-microbe interactions, low molecular weight metabolites, capillary electrophoresis-mass spectrometry, time-of-flight mass spectrometry, metabolite profiling
- MeSH terms
- Animals, Bacteria, Female, Intestinal Mucosa, Intestines, Male, Mice, Inbred BALB C, Specific Pathogen-Free Organisms, Mass Spectrometry, Electrophoresis, Capillary, Mice, Host-Pathogen Interactions, Metagenome, Metabolomics
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