Probiotic Bifidobacterium longum alters gut luminal metabolism through modification of the gut microbial community
Open accessAbstract
Probiotics are well known as health-promoting agents that modulate intestinal microbiota. However, the molecular mechanisms underlying this effect remain unclear. Using gnotobiotic mice harboring 15 strains of predominant human gut-derived microbiota (HGM), we investigated the effects of Bifidobacterium longum BB536 (BB536-HGM) supplementation on the gut luminal metabolism. Nuclear magnetic resonance (NMR)-based metabolomics showed significantly increased fecal levels of pimelate, a precursor of biotin and butyrate in the BB536-HGM group. In addition, the bioassay revealed significantly elevated fecal levels of biotin in the BB536-HGM group. Metatranscriptomic analysis of fecal microbiota followed by an in vitro bioassay indicated that the elevated biotin level was due to an alteration in metabolism related to biotin synthesis by Bacteroides caccae in this mouse model. Furthermore, the proportion of Eubacterium rectale, a butyrate producer, was significantly higher in the BB536-HGM group than in the group without B. longum BB536 supplementation. Our findings help to elucidate the molecular basis underlying the effect of B. longum BB536 on the gut luminal metabolism through its interactions with the microbial community.
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In vitro and in vivo studies have shown that modulating bifidobacterial levels through probiotic or prebiotic supplementation can change the overall composition and metabolism of the gut microbiota.
“In vitro and in vivo studies have shown that modulating bifidobacterial levels through probiotic or prebiotic supplementation can change the overall composition and metabolism of the gut microbiota (49–53).”
From Bifidobacteria and Their Health-Promoting Effects (2017) · cites this paper as [53]
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References 57 · Cited by 169
- Authors
- Sugahara, H., Odamaki, T., Fukuda, S., Kato, T., Xiao, J.-Z., Abe, F., Kikuchi, J., & Ohno, H.
- Affiliations
- RIKEN Center for Integrative Medical Sciences (JP)
- Morinaga Milk Industry Co., Ltd. (Japan) (JP)
- RIKEN Center for Sustainable Resource Science (JP)
- Corresponding author
- Sugahara, Hirosuke
- ORCID
- Venue
- Scientific Reports
- Published
- 2015-08-28
- Volume
- 5
- Issue
- 1
- Pages
- 13548-13548
- ISSN
- 2045-2322 (ISSN-L)
- PMC
- PMC4552000
- OpenAlex
- W2408795218
- Cited by
- 169
- References
- 57
- Citations by year
- 2026: 8 · 2025: 15 · 2024: 13 · 2023: 10 · 2022: 17 · 2021: 23 · 2020: 14 · 2019: 19 · 2018: 15 · 2017: 20 · 2016: 13 · 2015: 1
- FWCI
- 7.10
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Scientific Reports (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- Morinaga Milk Industry
- University of Tokyo
- Japan Society for the Promotion of Science (25513012, 26117725)
- RIKEN
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
- Keywords
- Bifidobacterium longum BB536, probiotics, gut microbiota, pimelate, biotin biosynthesis, Bacteroides caccae, butyrate, Eubacterium rectale, fecal metabolites, gnotobiotic mice
- MeSH terms
- Animals, Bifidobacterium, Biotin, Feces, Gene Expression Regulation, Germ-Free Life, Humans, Magnetic Resonance Spectroscopy, RNA, Messenger, Solubility, Species Specificity, Water, Probiotics, Gastrointestinal Tract, Mice, Metabolomics, Metabolome, Microbiota
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