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A DNA Mini-Barcoding System for Authentication of Processed Fish Products
Open accessAbstract
Species substitution is a form of seafood fraud for the purpose of economic gain. DNA barcoding utilizes species-specific DNA sequence information for specimen identification. Previous work has established the usability of short DNA sequences—mini-barcodes—for identification of specimens harboring degraded DNA. This study aims at establishing a DNA mini-barcoding system for all fish species commonly used in processed fish products in North America. Six mini-barcode primer pairs targeting short (127–314 bp) fragments of the cytochrome c oxidase I (CO1) DNA barcode region were developed by examining over 8,000 DNA barcodes from species in the U.S. Food and Drug Administration (FDA) Seafood List. The mini-barcode primer pairs were then tested against 44 processed fish products representing a range of species and product types. Of the 44 products, 41 (93.2%) could be identified at the species or genus level. The greatest mini-barcoding success rate found with an individual primer pair was 88.6% compared to 20.5% success rate achieved by the full-length DNA barcode primers. Overall, this study presents a mini-barcoding system that can be used to identify a wide range of fish species in commercial products and may be utilized in high throughput DNA sequencing for authentication of heavily processed fish products.
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Kimura, M. (1980) · Journal of Molecular Evolution · doi:10.1007/bf01731581 · Cited by 29,778
Cited by
Scaling up: A guide to high‐throughput genomic approaches for biodiversity analysis
Porter, T. M., & Hajibabaei, M. (2018) · Molecular Ecology · doi:10.1111/mec.14478 · Cited by 305
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Metadata
- Authors
- Shokralla, S., Hellberg, R. S., Handy, S. M., King, I., & Hajibabaei, M.
- Venue
- Scientific Reports
- Published
- 2015-10-30
- Volume
- 5
- Issue
- 1
- Pages
- 15894-15894
- ISSN
- 2045-2322 (ISSN-L)
- OpenAlex
- W2193447811
- Cited by
- 214
- References
- 1
- FWCI
- 5.40
- Type
- journal article
- Language
- en
- Open access
- gold
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- Shokralla, S., Hellberg, R. S., Handy, S. M., King, I., & Hajibabaei, M. (2015). A DNA Mini-Barcoding System for Authentication of Processed Fish Products. Scientific Reports. https://doi.org/10.1038/srep15894
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