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Cited by 136Neuroinflammation and Neurodegeneration MechanismsAutophagy in Disease and TherapyLipid metabolism and biosynthesis

Lipid-laden cells differentially distributed in the aging brain are functionally active and correspond to distinct phenotypes

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Shimabukuro, Marilia Kimie, Langhi, Larissa Gutman Paranhos, Cordeiro, Ingrid, Brito, José M., Batista, Claudia Maria de Castro, Mattson, Mark P., de Mello Coelho, Valeria·2016·Scientific Reports
DOI 10.1038/srep23795PMC PMC4814830
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Abstract

We characterized cerebral Oil Red O-positive lipid-laden cells (LLC) of aging mice evaluating their distribution, morphology, density, functional activities and inflammatory phenotype. We identified LLC in meningeal, cortical and neurogenic brain regions. The density of cerebral LLC increased with age. LLC presenting small lipid droplets were visualized adjacent to blood vessels or deeper in the brain cortical and striatal parenchyma of aging mice. LLC with larger droplets were asymmetrically distributed in the cerebral ventricle walls, mainly located in the lateral wall. We also found that LLC in the subventricular region co-expressed beclin-1 or LC3, markers for autophagosome or autophagolysosome formation and perilipin (PLIN), a lipid droplet-associated protein, suggesting lipophagic activity. Some cerebral LLC exhibited β galactosidase activity indicating a senescence phenotype. Moreover, we detected production of the pro-inflammatory cytokine TNF-α in cortical PLIN+ LLC. Some cortical NeuN+ neurons, GFAP+ glia limitans astrocytes, Iba-1+ microglia and S100β+ ependymal cells expressed PLIN in the aging brain. Our findings suggest that cerebral LLC exhibit distinct cellular phenotypes and may participate in the age-associated neuroinflammatory processes.

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  • Lipid droplets were induced in LPS-treated mouse hippocampal slice cultures and in the N9 microglia cell line.

    “Lipid droplets were also induced in LPS-treated mouse hippocampal slice cultures and in the N9 microglia cell line15,16.”

    From Lipid-droplet-accumulating microglia represent a dysfunctional and proinflammatory state in the aging brain (2020) · cites this paper as [15]

    The Results section describes lipid-laden cells in brain tissue from aging mice, and the Methods describe staining brain sections. Searches for LPS-treated hippocampal slice cultures and the N9 microglia cell line found no passage addressing either experiment in our copy. (LLM, conf=0.74)

    Experimental — accuracy not yet measured

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Authors
Shimabukuro, M. K., Langhi, L. G. P., Cordeiro, I., Brito, J. M., Batista, C. M. D. C., Mattson, M. P., & de Mello Coelho, V.
Affiliations
  • Universidade Federal do Rio de Janeiro (BR)
  • National Institutes of Health (US)
ORCID
Venue
Scientific Reports
Publisher
Springer Science and Business Media LLC
Published
2016-03-31
Volume
6
Issue
1
Pages
23795-23795
ISSN
2045-2322 (ISSN-L)
DOI
10.1038/srep23795
PMC
PMC4814830
OpenAlex
W2321285367
Cited by
136
References
61
Citations by year
2026: 11 · 2025: 14 · 2024: 19 · 2023: 22 · 2022: 15 · 2021: 24 · 2020: 15 · 2019: 8 · 2018: 6 · 2017: 2
FWCI
2.92
Type
journal article
Language
en
Open access
Licence
Locations
  • Scientific Reports (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Funders
  • Conselho Nacional de Desenvolvimento Científico e Tecnológico
  • Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro
  • National Institutes of Health
  • National Institute on Aging
Topics
  • Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
  • Health Sciences › Medicine › Epidemiology › Autophagy in Disease and Therapy
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Keywords
brain aging, lipid droplets, perilipin, lipophagy, autophagy, Beclin-1, LC3, cellular senescence, TNF-α, neuroinflammation, meningeal cells, subventricular zone, ependymal cells, microglia, astrocytes, striatum, brain ventricles
MeSH terms
Perilipin-1, Beclin-1, Aging, Animals, Astrocytes, Autophagy, beta-Galactosidase, Calcium-Binding Proteins, Cell Count, Cerebral Cortex, Female, Glial Fibrillary Acidic Protein, Histocytochemistry, Meninges, Mice, Inbred BALB C, Microfilament Proteins, Microtubule-Associated Proteins, Neurons, Phenotype, Tumor Necrosis Factor-alpha, Gene Expression, Microglia, Mice, Neurogenesis, S100 Calcium Binding Protein beta Subunit, Lipid Droplets

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