Circulatory shear flow alters the viability and proliferation of circulating colon cancer cells
Open accessAbstract
During cancer metastasis, circulating tumor cells constantly experience hemodynamic shear stress in the circulation. Cellular responses to shear stress including cell viability and proliferation thus play critical roles in cancer metastasis. Here, we developed a microfluidic approach to establish a circulatory microenvironment and studied circulating human colon cancer HCT116 cells in response to a variety of magnitude of shear stress and circulating time. Our results showed that cell viability decreased with the increase of circulating time, but increased with the magnitude of wall shear stress. Proliferation of cells survived from circulation could be maintained when physiologically relevant wall shear stresses were applied. High wall shear stress (60.5 dyne/cm 2), however, led to decreased cell proliferation at long circulating time (1 h). We further showed that the expression levels of β-catenin and c-myc, proliferation regulators, were significantly enhanced by increasing wall shear stress. The presented study provides a new insight to the roles of circulatory shear stress in cellular responses of circulating tumor cells in a physiologically relevant model and thus will be of interest for the study of cancer cell mechanosensing and cancer metastasis.
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Techniques that overcome abnormal vasculature in the tumor, such as nanoparticle drug delivery and drugs normalizing vascular network, were shown to be able to greatly improve anti-cancer treatment.
“In recent years, techniques which can overcome the abnormal vasculature in the tumor, such as nanoparticle drug delivery and drugs normalizing vascular network, were shown to be able to greatly improve anti-cancer treatment [108-113].”
From Microfluidic modelling of the tumor microenvironment for anti-cancer drug development (2019) · cites this paper as [113]
The Abstract and Discussion describe how circulatory shear stress affects colon cancer cell viability and proliferation, not treatments that overcome abnormal tumor vasculature. Searches for nanoparticle drug delivery and vascular normalization found no passage addressing whether those techniques improve anti-cancer treatment; no page numbers are available in our copy. (LLM, conf=0.75)
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- Authors
- Fan, R., Emery, T., Zhang, Y., Xia, Y., Sun, J., & Wan, J.
- Affiliations
- Rochester Institute of Technology (US)
- University of Illinois Chicago (US)
- Columbia University (US)
- Corresponding author
- Sun, Jun
- Wan, Jiandi
- Venue
- Scientific Reports
- Published
- 2016-06-03
- Volume
- 6
- Issue
- 1
- Pages
- 27073-27073
- ISSN
- 2045-2322 (ISSN-L)
- PMC
- PMC4891768
- OpenAlex
- W2468954576
- Cited by
- 103
- References
- 37
- Citations by year
- 2026: 3 · 2025: 7 · 2024: 11 · 2023: 12 · 2022: 14 · 2021: 15 · 2020: 11 · 2019: 16 · 2018: 11 · 2017: 3
- FWCI
- 6.89
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Scientific Reports (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- eScholarship (California Digital Library) (submittedVersion, cc-by)
- Funders
- Swim Across America
- National Institute of Diabetes and Digestive and Kidney Diseases (R01DK105118)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Health Sciences › Medicine › Oncology › Cancer Cells and Metastasis
- Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Bio-sensing Technologies
- Keywords
- circulating tumor cells, colon cancer, wall shear stress, hemodynamic shear stress, cell viability, cell proliferation, metastasis, β-catenin, c-Myc, mechanosensing, HCT116 cells
- MeSH terms
- Biomechanical Phenomena, Cell Survival, Humans, Models, Biological, Neoplastic Cells, Circulating, Rheology, Stress, Mechanical, Gene Expression, Proto-Oncogene Proteins c-myc, Mechanotransduction, Cellular, HCT116 Cells, Microfluidic Analytical Techniques, Cell Proliferation, beta Catenin
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