Cited by 46Plasma Applications and DiagnosticsRNA Interference and Gene DeliveryMicrobial Inactivation Methods

Intracellular ROS mediates gas plasma-facilitated cellular transfection in 2D and 3D cultures

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Xu, Dehui, Wang, Biqing, Xu, Yujing, Chen, Zeyu, Cui, Qinjie, Yang, Yanjie, Chen, Hailan, Kong, Michael G.·2016·Scientific Reports

Abstract

This study reports the potential of cold atmospheric plasma (CAP) as a versatile tool for delivering oligonucleotides into mammalian cells. Compared to lipofection and electroporation methods, plasma transfection showed a better uptake efficiency and less cell death in the transfection of oligonucleotides. We demonstrated that the level of extracellular aqueous reactive oxygen species (ROS) produced by gas plasma is correlated with the uptake efficiency and that this is achieved through an increase of intracellular ROS levels and the resulting increase in cell membrane permeability. This finding was supported by the use of ROS scavengers, which reduced CAP-based uptake efficiency. In addition, we found that cold atmospheric plasma could transfer oligonucleotides such as siRNA and miRNA into cells even in 3D cultures, thus suggesting the potential for unique applications of CAP beyond those provided by standard transfection techniques. Together, our results suggest that cold plasma might provide an efficient technique for the delivery of siRNA and miRNA in 2D and 3D culture models.

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Crossref · 10.1038/srep27872

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  • In a recent study, gas plasma-facilitated cellular transfection reached siRNA extinction efficiency of about 20% and plasmid transfection efficiency of less than 5% in a 3D culture system, compared with more than 80% and more than 40% in the present system.

    “Moreover, in a recent study (36) demonstrating the benefits of a novel gas plasma-facilitated cellular transfection method, siRNA extinction efficiency of about 20% (as compared to more than 80% in our system) and plasmid transfection efficiency of less than 5% (as compared to more than 40% in our system) were reached in a 3D culture system.”

    From Direct transfection of clonal organoids in Matrigel microbeads: a promising approach toward organoid-based genetic screens (2018) · cites this paper as [36]

    The Results (page unavailable) report gas plasma-facilitated delivery of siRNA in 3D cultures and relatively poor plasmid transfection in both 2D and 3D cultures. They do not state about 20% siRNA extinction or less than 5% plasmid transfection; the Methods describe measuring siRNA uptake, not siRNA extinction. The figures cited in the Results do not supply those percentages in our text copy. (LLM, conf=0.83)

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Authors
Xu, D., Wang, B., Xu, Y., Chen, Z., Cui, Q., Yang, Y., Chen, H., & Kong, M. G.
Affiliations
  • State Key Laboratory of Electrical Insulation and Power Equipment (CN)
  • First Affiliated Hospital of Xi'an Jiaotong University (CN)
  • Old Dominion University (US)
Corresponding author
  • Xu, Dehui
  • Kong, Michael G.
Venue
Scientific Reports
Published
2016-06-14
Volume
6
Issue
1
Pages
27872-27872
ISSN
2045-2322 (ISSN-L)
OpenAlex
W2436939943
Cited by
46
References
0
Citations by year
2026: 1 · 2025: 3 · 2024: 3 · 2023: 7 · 2022: 4 · 2021: 8 · 2020: 1 · 2019: 3 · 2018: 5 · 2017: 10 · 2016: 1
FWCI
5.50
Type
journal article
Language
en
Open access
Locations
  • Scientific Reports (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • ODU Digital Commons (Old Dominion University) (submittedVersion)
Funders
National Natural Science Foundation of China (51307135, 51221005)
Topics
  • Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Plasma Applications and Diagnostics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Microbial Inactivation Methods
Keywords
cold atmospheric plasma, reactive oxygen species, intracellular ROS, oligonucleotide delivery, siRNA, microRNA, cell membrane permeability, 3D cell culture, ROS scavengers, transfection efficiency
MeSH terms
Cell Membrane Permeability, DNA, Humans, Transfection, Reactive Oxygen Species, Electroporation, Cell Culture Techniques, RNA, Small Interfering, MicroRNAs, Intracellular Space, Cell Line, Tumor, Plasma Gases

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