Azole-resistant Aspergillus fumigatus harboring TR34/L98H, TR46/Y121F/T289A and TR53 mutations related to flower fields in Colombia
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Resistance to triazoles in Aspergillus fumigatus has been reported in azole-naive patients in Europe, Asia, Australia and North America. This resistance has been linked to fungicide-driven mutations in the cyp51A gene and its promoter region. We investigated the presence of environmental azole-resistant A. fumigatus strains related to the use of azole fungicides in Colombia. Soil samples were collected from flower beds, flower fields and public gardens from the outskirts, suburbs and city centre of Bogotá. Out of the 86 soil samples taken, 17 (19.8%) grew A. fumigatus of whom eight (9.3%) contained 40 strains able to grow on azole-containing itraconazole and/or voriconazole supplemented media. All but one triazole-resistant strains were isolated from soil samples collected from flower fields and flower beds (39/40). Importantly, the majority had the TR 46 /Y121F/T289A, TR 34 /L98H, and TR 53 molecular resistance mechanisms and one azole resistant strain had a wild-type cyp51A gene. Soil samples from flower fields and beds contained 4 azole fungicides (penconazole, difenoconazole, tetraconazole and tebuconazole) above the limit of detection. Our findings underline the need for extensive investigations to determine azole-resistant A. fumigatus prevalence in both clinical and environmental samples in other regions of Latin America.
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Duplications in the cyp51A gene promoter coupled with TR34/L98H and TR46/Y121F/T289A substitutions are becoming widespread in the environment associated with azole use in agriculture and are also isolated from hospital patients without previous antifungal exposure.
“Alarmingly, duplications in the cyp51A gene promoter coupled with specific amino acid substitutions, TR34/L98H and TR46/Y121F/T289A, are becoming widespread in the environment associated with azole use in agriculture, while also isolated from hospital patients without previous exposure to antifungal drugs19–22.”
From Molecular mechanisms governing antifungal drug resistance (2023) · cites this paper as [22]
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References 35 · Cited by 141
- Authors
- Alvarez-Moreno, C., Lavergne, R.-A., Hagen, F., Morio, F., Meis, J. F., & Le Pape, P.
- Affiliations
- Centre Hospitalier Universitaire de Nantes (FR)
- Canisius-Wilhelmina Ziekenhuis (NL)
- Radboud University Nijmegen (NL)
- Venue
- Scientific Reports
- Published
- 2017-03-30
- Volume
- 7
- Issue
- 1
- Pages
- 45631-45631
- ISSN
- 2045-2322 (ISSN-L)
- PMC
- PMC5372364
- OpenAlex
- W2599192510
- Cited by
- 141
- References
- 35
- Citations by year
- 2026: 17 · 2025: 14 · 2024: 10 · 2023: 12 · 2022: 5 · 2021: 23 · 2020: 16 · 2019: 19 · 2018: 14 · 2017: 11
- FWCI
- 12.63
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Scientific Reports (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Data Archiving and Networked Services (DANS) (submittedVersion, other-oa)
- HAL (submittedVersion, cc-by)
- Radboud Repository (Radboud University) (submittedVersion)
- Radboud Repository (Radboud University) (submittedVersion, other-oa)
- Greater South Information System
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Agricultural and Biological Sciences › Ecology, Evolution, Behavior and Systematics › Fungal Plant Pathogen Control
- Physical Sciences › Environmental Science › Pollution › Pharmaceutical and Antibiotic Environmental Impacts
- Keywords
- Aspergillus fumigatus, triazole resistance, cyp51A mutations, TR34/L98H, azole fungicides, soil fungi, flower fields, environmental resistance, itraconazole, voriconazole, penconazole, difenoconazole, tetraconazole, tebuconazole, Colombia, Bogotá
- MeSH terms
- Aspergillus fumigatus, Azoles, Colombia, Fungicides, Industrial, Genes, Fungal, Genotype, Soil Microbiology, Drug Resistance, Fungal
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