Identification of pro-inflammatory CD205+ macrophages in livers of hepatitis B virus transgenic mice and patients with chronic hepatitis B
Open accessAbstract
Hepatic macrophages play a central role in disease pathogenesis during hepatitis B virus (HBV) infection. Our previous study found that CD205+ macrophages in the liver of hepatitis B surface antigen transgenic (HBs-Tg) mice increased significantly compared with those in wild-type mice, and these increased CD205+ macrophages were involved in CpG-oligodeoxynucleotide-induced liver injury in HBs-Tg mice. Here, we analysed the phenotype and function of CD205+ macrophages derived from the liver of HBs-Tg mice and patients with chronic hepatitis B (CHB). We found that HBs-Tg mice-derived hepatic macrophages produced larger amounts of pro-inflammatory cytokines, including IL-6, IL-12, TNF-α, and of the anti-inflammatory cytokine IL-10 after stimulation with CpG-oligodeoxynucleotides or commensal bacteria DNA than B6 mice-derived hepatic macrophages. Furthermore, hepatic CD205+ macrophages from HBs-Tg mice showed an activated phenotype and expressed higher levels of inflammatory cytokine genes, chemokine genes, and phagocytosis-related genes than hepatic CD205− macrophages. In addition, CD205+ macrophages displayed an inflammatory phenotype and were increased in the liver of patients with CHB compared with those in healthy controls. Our data suggest that hepatic CD205+ macrophages are a unique pro-inflammatory subset observed during HBV infection. Thus, development of intervention targeting these cells is warranted for immunotherapy of HBV-induced liver diseases.
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Human CD205+ macrophages exhibit an activated phenotype (elevated HLA-DR, CD14, and CD16) and produce higher levels of inflammatory cytokines such as TNF-α and IL-12, likely contributing to sustained inflammation and liver injury in HBV infection.
“These human CD205+ macrophages exhibit an activated phenotype (elevated HLA-DR, CD14, and CD16) and produce higher levels of inflammatory cytokines such as TNF-α and IL-12, underscoring their likely contribution to sustained inflammation and liver injury in the clinical setting of HBV infection (53).”
From Macrophage duality in liver diseases: heterogeneity, plasticity, and the quest for precision therapeutics (2026) · cites this paper as [53]
The pro-inflammatory CD205+ macrophage subset is expanded in the livers of patients with chronic hepatitis B.
“Importantly, this pro-inflammatory CD205+ macrophage subset is also expanded in the livers of patients with chronic hepatitis B (CHB).”
From Macrophage duality in liver diseases: heterogeneity, plasticity, and the quest for precision therapeutics (2026) · cites this paper as [53]
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- Authors
- Yong, L., Li, M., Gao, Y., Deng, Y., Liu, W., Huang, D., Ren, C., Liu, M., Shen, J., & Hou, X.
- Affiliations
- Anhui Medical University (CN)
- Corresponding author
- Hou, Xin
- Venue
- Scientific Reports
- Published
- 2017-04-24
- Volume
- 7
- Issue
- 1
- Pages
- 46765-46765
- ISSN
- 2045-2322 (ISSN-L)
- PMC
- PMC5402278
- OpenAlex
- W2608749031
- Cited by
- 12
- References
- 36
- Citations by year
- 2026: 2 · 2024: 1 · 2022: 1 · 2021: 2 · 2020: 2 · 2019: 1 · 2018: 3
- FWCI
- 1.29
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Scientific Reports (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (81402474, 81571540)
- Natural Science Foundation of Anhui Province
- Topics
- Health Sciences › Medicine › Epidemiology › Hepatitis B Virus Studies
- Health Sciences › Medicine › Hepatology › Liver physiology and pathology
- Life Sciences › Immunology and Microbiology › Immunology › Immune cells in cancer
- Keywords
- hepatitis B virus, chronic hepatitis B, hepatic macrophages, inflammatory macrophages, CpG oligodeoxynucleotides, cytokine production, interleukin-6, IL-12, TNF-α, IL-10, macrophage activation, chemokines, phagocytosis, liver inflammation
- MeSH terms
- Animals, Female, Hepatitis B virus, Humans, Liver, Macrophages, Male, Mice, Inbred C57BL, Mice, Transgenic, Middle Aged, Oligodeoxyribonucleotides, Receptors, Cell Surface, Antigens, CD, Minor Histocompatibility Antigens, Gene Expression, Cytokines, Inflammation Mediators, Hepatitis B, Chronic, Lectins, C-Type
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