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A water-soluble tetrazolium salt useful for colorimetric cell viability assay
Abstract
The application of a tetrazolium salt, 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H tetrazolium, monosodium salt (WST-8), to cell viability assays and in vitro drug sensitivity tests is described. With a higher sensitivity as a chromogenic indicator for cell viability compared with conventional tetrazolium salts, WST-8 produced results of cell viability and IC50 values that were in good agreement, respectively, with the MTT method and [3H]thymidine uptake method.
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Cited by
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Tamura, T., Ueda, T., Goto, T., Tsukidate, T., Shapira, Y., Nishikawa, Y., Fujisawa, A., & Hamachi, I. (2018) · Nature Communications · doi:10.1038/s41467-018-04343-0 · Cited by 242
Metadata
- Authors
- Tominaga, H., Ishiyama, M., Ohseto, F., Sasamoto, K., Hamamoto, T., Suzuki, K., & Watanabe, M.
- Venue
- Analytical Communications
- Publisher
- Royal Society of Chemistry (RSC)
- Published
- 1999
- Volume
- 36
- Issue
- 2
- Pages
- 47-50
- ISSN
- 1359-7337 (ISSN-L)
- 1364-5536
- OpenAlex
- W2053281570
- Cited by
- 716
- References
- 0
- FWCI
- 2.15
- Type
- journal article
- Language
- en
- Open access
- closed
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- Tominaga, H., Ishiyama, M., Ohseto, F., Sasamoto, K., Hamamoto, T., Suzuki, K., & Watanabe, M. (1999). A water-soluble tetrazolium salt useful for colorimetric cell viability assay. Analytical Communications. https://doi.org/10.1039/a809656b
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