The emerging process of Top Down mass spectrometry for protein analysis: biomarkers, protein-therapeutics, and achieving high throughput
Open accessAbstract
Top Down mass spectrometry (MS) has emerged as an alternative to common Bottom Up strategies for protein analysis. In the Top Down approach, intact proteins are fragmented directly in the mass spectrometer to achieve both protein identification and characterization, even capturing information on combinatorial post-translational modifications. Just in the past two years, Top Down MS has seen incremental advances in instrumentation and dedicated software, and has also experienced a major boost from refined separations of whole proteins in complex mixtures that have both high recovery and reproducibility. Combined with steadily advancing commercial MS instrumentation and data processing, a high-throughput workflow covering intact proteins and polypeptides up to 70 kDa is directly visible in the near future.
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References 52 · Cited by 99
- Authors
- Kellie, J. F., Tran, J. C., Lee, J. E., Ahlf, D. R., Thomas, H. M., Ntai, I., Catherman, A. D., Durbin, K. R., Zamdborg, L., Vellaichamy, A., Thomas, P. M., & Kelleher, N. L.
- Affiliations
- University of Illinois Urbana-Champaign (US)
- Corresponding author
- Kelleher, Neil L
- ORCID
- Venue
- Molecular BioSystems
- Publisher
- Oxford University Press (OUP)
- Published
- 2010-03-29
- Volume
- 6
- Issue
- 9
- Pages
- 1532-1539
- ISSN
- 1742-2051 (ISSN-L)
- 1742-206X
- PMC
- PMC3115741
- OpenAlex
- W2119425965
- Cited by
- 99
- References
- 52
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 1 · 2023: 4 · 2022: 3 · 2021: 5 · 2020: 3 · 2019: 2 · 2018: 4 · 2017: 11 · 2016: 4 · 2015: 9 · 2014: 14 · 2013: 14 · 2012: 10
- FWCI
- 5.28
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Molecular BioSystems (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- David and Lucile Packard Foundation
- National Institute of General Medical Sciences (R01GM067193, GM 067193)
- Topics
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Physical Sciences › Chemistry › Spectroscopy › Mass Spectrometry Techniques and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
- Keywords
- top-down mass spectrometry, protein analysis, biomarkers, therapeutic proteins, post-translational modification, high-throughput proteomics, protein separation, polypeptides
- MeSH terms
- HeLa Cells, Humans, Proteins, Mass Spectrometry, Biomarkers, Proteomics
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