Protein-engineered scaffolds for in vitro 3D culture of primary adult intestinal organoids
Open accessAbstract
A family of recombinant, protein-engineered mimics of the extracellular matrix were used to identify the microenvironmental factors that impact the 3D culture efficiency of primary adult intestinal organoids.
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Inhibition of MMP activity reduced organoid-forming efficiency in the stiffer engineered hydrogel matrices, suggesting that secretion of degradative enzymes in adult intestinal organoids may be a response to overly stiff conditions.
“Inhibition of MMP activity reduced organoid-forming efficiency in the stiffer engineered hydrogel matrices, suggesting that secretion of degradative enzymes in adult intestinal organoids may be a response to overly stiff conditions191.”
From Towards organoid culture without Matrigel (2021) · cites this paper as [191]
A very soft matrix with an elastic modulus of 180 Pa promoted intestinal organoid-forming efficiencies comparable to those observed in collagen I-based matrices.
“In another follow-up study, a very soft matrix with an elastic modulus of 180 Pa promoted intestinal organoid-forming efficiencies comparable to those observed in collagen I-based matrices.”
From Towards organoid culture without Matrigel (2021) · cites this paper as [191]
MMP activity was significantly higher in the stiffer matrices.
“Interestingly, MMP activity was significantly higher in the stiffer matrices.”
From Towards organoid culture without Matrigel (2021) · cites this paper as [191]
Organoid-forming efficiency was higher when the engineered ECM proteins contained 3.2 mM RGD peptide, compared to no RGD.
“Organoid-forming efficiency was higher when the engineered ECM proteins contained 3.2 mM RGD peptide, compared to no RGD.”
From Towards organoid culture without Matrigel (2021) · cites this paper as [191]
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References 56 · Cited by 66
- Authors
- DiMarco, R. L., Dewi, R. E., Bernal, G., Kuo, C., & Heilshorn, S. C.
- Affiliations
- Stanford University (US)
- Stanford Medicine (US)
- Corresponding author
- Heilshorn, Sarah C.
- Venue
- Biomaterials Science
- Publisher
- Royal Society of Chemistry (RSC)
- Published
- 2015
- Volume
- 3
- Issue
- 10
- Pages
- 1376-1385
- ISSN
- 2047-4830 (ISSN-L)
- 2047-4849
- PMC
- PMC9063856
- OpenAlex
- W2158683031
- Cited by
- 66
- References
- 56
- Citations by year
- 2026: 2 · 2025: 11 · 2024: 5 · 2023: 9 · 2022: 4 · 2021: 16 · 2020: 3 · 2019: 4 · 2018: 4 · 2017: 6 · 2016: 2
- FWCI
- 4.03
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Biomaterials Science (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Science Foundation (DEG-1147470)
- National Institutes of Health (1DP2-OD006477-01, 1R21-EB018407-01, 1U19AI116484-01, R01 DK085720-01)
- Stanford University (BioX Graduate Fellowship)
- Topics
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Keywords
- intestinal organoids, extracellular matrix scaffolds, air-liquid interface culture, submerged culture, stiffness, type I collagen, matrix remodeling, biomechanical cues, biochemical cues, organoid formation, intestinal tissue engineering, microenvironmental cues
- MeSH terms
- Animals, Cell Adhesion, Cell Differentiation, Extracellular Matrix, Intestines, Mice, Inbred C57BL, Organ Culture Techniques, Organoids, Tissue Engineering, Collagen Type I, Mice
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