Chemotaxis-driven assembly of endothelial barrier in a tumor-on-a-chip platform
Open accessAbstract
Chemotaxis-driven assembly of an endothelial barrier around tumor cells within a microfluidics device.
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At concentrations of 10 and 100 μg/mL, DOX destroyed the endothelial barrier, decreased the size of spheroids, and caused a darkening of the MCF7 spheroids.
“At concentrations of 10 and 100 μg/mL, DOX destroyed the endothelial barrier, decreased the size of spheroids, and caused a darkening of the MCF7 spheroids”
From The Tumor-on-Chip: Recent Advances in the Development of Microfluidic Systems to Recapitulate the Physiology of Solid Tumors (2019) · cites this paper as [50]
The Results section reports that MCF7 spheroids exposed to 10 and 100 μg/mL doxorubicin became smaller and darker, and that the endothelial barrier disappeared after doxorubicin exposure. However, the Discussion specifies barrier loss at high doses (>10 μg/mL), so the paper does not establish barrier destruction at 10 μg/mL. (LLM, conf=0.88)
Experimental — accuracy not yet measuredHUVECs chemotactically migrated from the bulk of the hydrogel towards its perimeter and spontaneously developed an endothelial barrier around the GelMA construct.
“Over time, the HUVECs chemotactically migrated from the bulk of the hydrogel towards its perimeter and spontaneously developed an endothelial barrier around the GelMA construct”
From The Tumor-on-Chip: Recent Advances in the Development of Microfluidic Systems to Recapitulate the Physiology of Solid Tumors (2019) · cites this paper as [50]
Starting from homogeneity, HUVEC density varied in different areas within the constructs over time.
“As a result, starting from homogeneity, the HUVEC density varied in different areas within the constructs over time”
From The Tumor-on-Chip: Recent Advances in the Development of Microfluidic Systems to Recapitulate the Physiology of Solid Tumors (2019) · cites this paper as [50]
The size of tumor spheroids, as determined by projected area, increased over time.
“The size of the tumor spheroids, as determined by their projected area, also increased over time”
From The Tumor-on-Chip: Recent Advances in the Development of Microfluidic Systems to Recapitulate the Physiology of Solid Tumors (2019) · cites this paper as [50]
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Cites
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Trujillo-de Santiago, G., Flores-Garza, B. G., Tavares-Negrete, J. A., Lara-Mayorga, I. M., González-Gamboa, I., Zhang, Y. S., Rojas-Martínez, A., Ortiz-López, R., & Álvarez, M. M. (2019) · Materials · doi:10.3390/ma12182945 · Cited by 184 · see citing works
“[50] developed a tumorous microtissue encapsulated by an endothelial barrier composed of HUVECs.”
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Self-citationCancer-on-a-chip systems at the frontier of nanomedicine
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3D Bioprinting of Tumor Models for Cancer Research
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- Authors
- Aung, A., Theprungsirikul, J., Lim, H. L., & Varghese, S.
- Affiliations
- University of California San Diego (US)
- Corresponding author
- Varghese, Shyni
- Venue
- Lab on a Chip
- Publisher
- Royal Society of Chemistry (RSC)
- Published
- 2016
- Volume
- 16
- Issue
- 10
- Pages
- 1886-1898
- ISSN
- 1473-0189 (ISSN-L)
- 1473-0197
- PMC
- PMC4862883
- OpenAlex
- W2340148718
- Cited by
- 50
- References
- 3
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 9 · 2023: 4 · 2022: 2 · 2021: 5 · 2020: 6 · 2019: 6 · 2018: 7 · 2017: 7 · 2016: 1
- FWCI
- 3.37
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Lab on a Chip (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institute of Arthritis and Musculoskeletal and Skin Diseases (R01 AR063184-02)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
- Keywords
- tumor-on-a-chip, chemotaxis, microfluidics, gelatin methacryloyl hydrogel, tumor spheroids, endothelial cell migration, morphogen gradients, drug screening, doxorubicin
- MeSH terms
- Cadherin 5, Cell Movement, Chemotaxis, Dose-Response Relationship, Drug, Doxorubicin, Drug Screening Assays, Antitumor, Gelatin, Humans, Methacrylates, Antigens, CD, Cadherins, Spheroids, Cellular, Coculture Techniques, Cell Culture Techniques, Endothelial Cells, Microfluidics, Lab-On-A-Chip Devices, Tumor Microenvironment, Human Umbilical Vein Endothelial Cells, MCF-7 Cells
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