Photo-cleavable nucleotides for primer free enzyme mediated DNA synthesis.
Abstract
The synthesis, characterization and potential application of eight 3'-O modified 2'-deoxyribonucleoside triphosphates (dNTPs) are discussed. These nucleotide analogues are modified by capping the 3'-OH with a photolabile protecting group which can temporarily cease DNA strand growth and can smoothly reinitiate the growth by the photodecomposition of the protecting group and setting the 3'-OH of dNTPs free to propagate. The synthesis of 3'-O-(2-nitrobenzyl)-2'-deoxyribonucleoside triphosphates (NB-dNTPs) and 3'-O-(4,5-dimethoxy-2-nitrobenzyl)-2'-deoxyribonucleoside triphosphates (DMNB-dNTPs) is discussed in detail with structural confirmation using NMR. The UV-cleaving studies are monitored and quantified using LCMS and (1)H NMR spectral traces. The synthesised nucleotides are employed for terminating and reinitiating template-less DNA synthesis, using primer independent Terminal Deoxynucleotidyl Transferase (TdT) enzyme. The use of this photolabile nucleotide in one step stop-start DNA synthesis is a novel strategy towards the precise assembly of dNTPs with the potential to reinforce present technologies.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The citation lists haven’t been fetched yet
References 15 · Cited by 50
- Authors
- Mathews, A. S., Yang, H., & Montemagno, C.
- Affiliations
- University of Alberta (CA)
- Corresponding author
- Montemagno, Carlo
- Venue
- Organic & Biomolecular Chemistry
- Publisher
- Royal Society of Chemistry (RSC)
- Published
- 2016
- Volume
- 14
- Issue
- 35
- Pages
- 8278-8288
- ISSN
- 1477-0520 (ISSN-L)
- 1477-0539
- OpenAlex
- W2518147233
- Cited by
- 50
- References
- 15
- Citations by year
- 2026: 4 · 2025: 10 · 2024: 10 · 2023: 5 · 2022: 6 · 2021: 6 · 2020: 3 · 2019: 2 · 2018: 3 · 2017: 1
- FWCI
- 1.04
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Organic & Biomolecular Chemistry (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Alberta Innovates - Technology Futures
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced biosensing and bioanalysis techniques
- Physical Sciences › Materials Science › Materials Chemistry › Photochromic and Fluorescence Chemistry
- Keywords
- photolabile protecting groups, terminal deoxynucleotidyl transferase, nucleotide synthesis
- MeSH terms
- DNA, DNA-Directed DNA Polymerase, DNA Replication, Magnetic Resonance Spectroscopy, Mass Spectrometry, Substrate Specificity, Electrophoresis, Gel, Two-Dimensional, DNA Primers, DNA Cleavage, Photochemical Processes
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
