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Cited by 167Microbial Metabolic Engineering and BioproductionAlgal biology and biofuel productionPhotosynthetic Processes and Mechanisms

Modular engineering for efficient photosynthetic biosynthesis of 1-butanol from CO 2 in cyanobacteria

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Liu, Xufeng, Miao, Rui, Lindberg, Pia, Lindblad, Peter·2019·Energy & Environmental Science
DOI 10.1039/c9ee01214a
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Abstract

Systematic modular engineering of a photosynthetic microorganism resulted in cells with significant levels and rates of 1-butanol production directly from CO 2 .

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  • Achieving the highest reported cyanobacterial 1-butanol titre of 4.8 g/L in Synechocystis sp. PCC 6803 required coordinated expression of CoA-dependent pathway genes, rewiring carbon flux toward acetyl-CoA, and repressing competing pathways; no single intervention was sufficient.

    “Liu et al. demonstrated in Synechocystis sp. PCC 6803 that achieving the highest reported cyanobacterial 1-butanol titre of 4.8 g/L required simultaneous attention to all three of these dimensions: coordinated expression of the CoA-dependent pathway genes was optimised through systematic tuning of promoters and RBS sequences; carbon flux was rewired toward the acetyl-CoA precursor through mod”

    From Cyanobacteria as a Photosynthetic Chassis for Metabolic Pathway Engineering with Heterologous Gene Expression (2026) · cites this paper as [144]

    The Conclusions and Long-term cultivation sections report that sequential, combined engineering of Synechocystis PCC 6803 reached 4.8 g/L, described as the highest cyanobacterial 1-butanol production reported at the time. The 5′-region optimization and carbon-flux sections support expression tuning, increased acetyl-CoA supply, and blocking competing routes. But the paper describes testing promoters and translational elements, not systematically tuning RBS sequences; it also identifies 4.8 g/L as a cumulative titer during long-term cultivation, a qualification the claim omits. (LLM, conf=0.87)

    Experimental — accuracy not yet measured

  • 1-butanol production reached titers of 300 mg/L, and bioethanol titers of up to 5.5 g/L were reported.

    “While 1-butanol production reached titers of 300 mg/L, bioethanol titers of up to 5.5 g/L were reported [77–79].”

    From The potential of biofuels from first to fourth generation (2023) · cites this paper as [79]

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References 63 · Cited by 167

Research works printed but not linked: 63

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Authors
Liu, X., Miao, R., Lindberg, P., & Lindblad, P.
Affiliations
Uppsala University (SE)
Corresponding author
Lindblad, Peter
ORCID
Venue
Energy & Environmental Science
Publisher
Royal Society of Chemistry (RSC)
Published
2019
Volume
12
Issue
9
Pages
2765-2777
ISSN
  • 1754-5692 (ISSN-L)
  • 1754-5706
DOI
10.1039/c9ee01214a
OpenAlex
W2956457960
Cited by
167
References
63
Citations by year
2026: 18 · 2025: 14 · 2024: 13 · 2023: 27 · 2022: 30 · 2021: 39 · 2020: 25 · 2019: 1
FWCI
7.86
Type
journal article
Language
en
Open access
Licence
Locations
  • Energy & Environmental Science (publishedVersion, cc-by-nc)
  • KTH Publication Database DiVA (KTH Royal Institute of Technology) (submittedVersion, other-oa)
Funders
Horizon 2020 Framework Programme (640720)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
  • Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
Keywords
1-butanol production, cyanobacteria, CO2 fixation, microbial cell factories, biofuel production

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