Magnetic-enhanced fluorescence sensing of tumor miRNA by combination of MNPs@PDA with duplex specific nuclease
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A simple and sensitive fluorescence biosensor has been developed for detection of miRNA-141 by combining the magnetic nanoparticles coated with poly-dopamine (MNPs@PDA) with duplex specific nuclease (DSN).
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Upon target-probe hybridization, increased probe rigidity leads to partial detachment from the PDA surface, resulting in fluorescence recovery.
“Upon target–probe hybridization, increased probe rigidity leads to partial detachment from the PDA surface, resulting in fluorescence recovery.”
From Polydopamine-Based Biomaterials in Oral Medicine: Applications, Mechanisms, and Translational Challenges (2026) · cites this paper as [88]
The Results and discussion section (Feasibility study of the method; page not provided) says target hybridization releases the DNA probe from MNPs@PDA and recovers fluorescence a little. The Introduction also says the resulting duplex has weak affinity for MNPs@PDA. Neither passage attributes the release to increased probe rigidity. (LLM, conf=0.86)
Experimental — accuracy not yet measuredThe π-π absorption band of PDA extends into the near-infrared region, enabling formation of efficient quenching pairs with fluorescent dyes such as Cy5 and Alexa 647, with a Förster radius of approximately 3.2 nm.
“In fluorescence-based sensing pathways, the π–π absorption band of PDA extends into the near-infrared region, enabling the formation of efficient quenching pairs with commonly used fluorescent dyes, such as Cy5 and Alexa 647, with a Förster radius of approximately 3.2 nm.”
From Polydopamine-Based Biomaterials in Oral Medicine: Applications, Mechanisms, and Translational Challenges (2026) · cites this paper as [88]
The Introduction (page unavailable) says PDA nanoparticles weaken fluorescence through an inner-filter effect, and the Feasibility study (page unavailable) reports a weak signal from FAM-labeled DNA bound to MNPs@PDA. The copy does not describe a near-infrared π–π absorption band, quenching pairs with Cy5 or Alexa 647, or a Förster radius of approximately 3.2 nm. (LLM, conf=0.87)
Experimental — accuracy not yet measuredFluorescence recovery efficiency exhibits a linear correlation with target concentration over the range of 10 fM to 1 nM, with a correlation coefficient R2 exceeding 0.99.
“Quantitative analysis demonstrates that the fluorescence recovery efficiency exhibits a linear correlation with target concentration over the range of 10 fM to 1 nM, with a correlation coefficient R2 exceeding 0.99.88”
From Polydopamine-Based Biomaterials in Oral Medicine: Applications, Mechanisms, and Translational Challenges (2026) · cites this paper as [88]
The Detection of miRNA section reports a linear relationship between fluorescence signal and target concentration with R² values of 0.9906 and 0.9933. It does not support the claimed 10 fM to 1 nM range: the reported ranges are 10 pM to 5 nM without magnetic separation and 5 pM to 5 nM with it. No page number is available in our copy. (LLM, conf=0.90)
Experimental — accuracy not yet measuredIn vitro tumor cell studies and selected animal tumor models support NIR-mediated photothermal therapy, drug loading, controlled release, and chemo-photothermal synergistic effects for PDA-based oral tumor therapy.
“In vitro tumor cell studies and selected animal tumor models support NIR-mediated photothermal therapy, drug loading, controlled release, and chemo-photothermal synergistic effects.”
From Polydopamine-Based Biomaterials in Oral Medicine: Applications, Mechanisms, and Translational Challenges (2026) · cites this paper as [88]
The Conclusions (page unavailable) report miRNA detection in human cells and describe MNPs@PDA only as promising candidates for controlled targeted drug delivery. Searches for NIR photothermal therapy, drug loading, controlled release, and animal tumor models found no passage reporting the claimed therapeutic studies or effects. (LLM, conf=0.74)
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Sorted by citations
Yang, Z., Song, W., Lv, L., Zhai, S., Liu, Y., Cui, H., Li, X., Gao, F., & Chu, S. (2026) · International Journal of Nanomedicine · doi:10.2147/ijn.s611922
“88 Taken together, the roles of PDA films at sensing interfaces can be conceptually summarized as dual functions of molecular anchoring and signal transduction.”
- Authors
- Sun, Y., Wang, C., Tang, L., Zhang, Y., & Zhang, G.-J.
- Affiliations
- Hubei University of Chinese Medicine (CN)
- School of Laboratory Medicine
- Corresponding author
- Zhang, Yulin
- Venue
- RSC Advances
- Publisher
- Royal Society of Chemistry (RSC)
- Published
- 2021
- Volume
- 11
- Issue
- 5
- Pages
- 2968-2975
- ISSN
- 2046-2069 (ISSN-L)
- PMC
- PMC8693806
- OpenAlex
- W3121070752
- Cited by
- 22
- References
- 0
- Citations by year
- 2026: 3 · 2025: 1 · 2024: 4 · 2023: 4 · 2022: 7 · 2021: 3
- FWCI
- 1.41
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- RSC Advances (publishedVersion, cc-by-nc)
- PubMed Central (publishedVersion, cc-by-nc)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (21974035)
- Hubei Provincial Department of Education (Q20162007)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced biosensing and bioanalysis techniques
- Physical Sciences › Engineering › Biomedical Engineering › Biosensors and Analytical Detection
- Physical Sciences › Chemistry › Spectroscopy › Molecular Sensors and Ion Detection
- Keywords
- magnetic nanoparticles, polydopamine, duplex-specific nuclease, label-free detection
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