CoChat
Sign Up
Cited by 45Alzheimer's disease research and treatmentsNeuroinflammation and Neurodegeneration MechanismsAutophagy in Disease and Therapy

Monitoring phagocytic uptake of amyloid β into glial cell lysosomes in real time

Open access
Prakash, Priya, Jethava, Krupal P., Korte, Nils, Izquierdo, Pablo, Favuzzi, Emilia, Rose, Indigo V. L., Guttenplan, Kevin A., Manchanda, Palak +6 more·2021·Chemical Science
DOI 10.1039/d1sc03486cPMC PMC8372545

Published version of a preprint

Open this paper in CoChat — free accountOpen PDF

Abstract

Glial cell phagocytosis of pH-dependent amyloid-β, Aβ pH , in live and fixed cultures, brain tissue sections, retina, cortex and in live animals useful for studying function in health and disease.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1039/d1sc03486c

Cites

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • The aggregated states of the Aβ1-42 peptides have been thoroughly characterized using atomic force microscopy and western blotting in previous papers.

    “The aggregated states of the Aβ1–42 peptides have been thoroughly characterized using atomic force microscopy and western blotting in our previous papers.100,47”

    From Amyloid-β induces lipid droplet-mediated microglial dysfunction via the enzyme DGAT2 in Alzheimer’s disease (2025) · cites this paper as [47]

    The Results section (page unavailable) reports AFM characterization of Aβ1–42 oligomers, and the Methods section says the AFM analysis followed previously published protocols. The passages do not establish that previous papers thoroughly characterized the peptides’ aggregated states using both AFM and western blotting; western blotting is not documented in the retrieved passages. (LLM, conf=0.85)

    Experimental — accuracy not yet measured

  • AβpH has been thoroughly characterized in vitro in primary cell culture and in vivo in a previous study.

    “AβpH is a pH-sensitive fluorophore conjugate of Aβ1–42 peptide that is generated within our laboratory and has been thoroughly characterized in vitro in primary cell culture as well as in vivo experiments in our previous study.47”

    From Amyloid-β induces lipid droplet-mediated microglial dysfunction via the enzyme DGAT2 in Alzheimer’s disease (2025) · cites this paper as [47]

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

The citation lists haven’t been fetched yet

References 62 · Cited by 45

Research works printed but not linked: 59

  1. Zuchero J. B. Barres B. A. Development 2015 142 3805 3809 26577203 PMC4712885
  2. Li Q. Barres B. A. Nat. Rev. Immunol. 2018 18 225 242 29151590
  3. Nimmerjahn A. Kirchhoff F. Helmchen F. Science 2005 308 1314 1318 15831717
  4. Bachstetter A. D. Van Eldik L. J. Schmitt F. A. Neltner J. H. Ighodaro E. T. Webster S. J. Patel E. Abner E. L. Kryscio R. J. Nelson P. T. Acta Neuropathol. Commun. 2015 3 32 26001591 PMC4489160
  5. Liddelow S. A. Guttenplan K. A. Clarke L. E. Bennett F. C. Bohlen C. J. Schirmer L. Bennett M. L. Münch A. E. Chung W.-S. Peterson T. C. Wilton D. K. Frouin A. Napier B. A. Panicker N. Kumar M. Buckwalter M. S. Rowitch D. H. Dawson V. L. Dawson T. M. Stevens B. Barres B. A. Nature 2017 541 481 487 28099414 PMC5404890
  6. Lloyd A. F. Miron V. E. Nat. Rev. Neurol. 2019 15 447 458 31256193
  7. Hanke M. L. Kielian T. Clin. Sci. 2011 121 367 387 PMC4231819 21745188
  8. Okun E. Mattson M. P. V Arumugam T. NeuroMol. Med. 2010 12 164 178 PMC2878892 19844812
  9. Husemann J. Loike J. D. Anankov R. Febbraio M. Silverstein S. C. Glia 2002 40 195 205 12379907
  10. Hong S. Stevens B. Dev. Cell 2016 38 126 128 27459063
  11. Canton J. J. Leukoc. Biol. 2014 96 729 738 24868088
  12. Wakida N. M. Cruz G. M. S. Ro C. C. Moncada E. G. Khatibzadeh N. Flanagan L. A. Berns M. W. PLoS One 2018 13 4 e0196153 29708987 PMC5927416
  13. Chung W.-S. Clarke L. E. Wang G. X. Stafford B. K. Sher A. Chakraborty C. Joung J. Foo L. C. Thompson A. Chen C. Smith S. J. Barres B. A. Nature 2013 504 394 400 24270812 PMC3969024
  14. Doherty J. Logan M. A. Tasdemir O. E. Freeman M. R. J. Neurosci. 2009 29 4768 4781 19369546 PMC2674269
  15. Fuentes-Medel Y. Logan M. A. Ashley J. Ataman B. Budnik V. Freeman M. R. PLoS Biol. 2009 7 8 e1000184 19707574 PMC2724735
  16. Morizawa Y. M. Hirayama Y. Ohno N. Shibata S. Shigetomi E. Sui Y. Nabekura J. Sato K. Okajima F. Takebayashi H. Okano H. Koizumi S. Nat. Commun. 2017 8 28 28642575 PMC5481424
  17. Nortley R. Korte N. Izquierdo P. Hirunpattarasilp C. Mishra A. Jaunmuktane Z. Kyrargyri V. Pfeiffer T. Khennouf L. Madry C. Gong H. Richard-Loendt A. Huang W. Saito T. Saido T. C. Brandner S. Sethi H. Attwell D. Science 2019 365 eaav9518 31221773 PMC6658218
  18. Lei M. Xu H. Li Z. Wang Z. O'Malley T. T. Zhang D. Walsh D. M. Xu P. Selkoe D. J. Li S. Neurobiol. Dis. 2016 85 111 121 26525100 PMC4778388
  19. Sato C. Barthélemy N. R. Mawuenyega K. G. Patterson B. W. Gordon B. A. Jockel-Balsarotti J. Sullivan M. Crisp M. J. Kasten T. Kirmess K. M. Kanaan N. M. Yarasheski K. E. Baker-Nigh A. Benzinger T. L. S. Miller T. M. Karch C. M. Bateman R. J. Neuron 2018 97 1284 1298.e7 29566794 PMC6137722
  20. Long J. M. Holtzman D. M. Cell 2019 179 312 339 31564456 PMC6778042
  21. Heneka M. T. Carson M. J. El Khoury J. Landreth G. E. Brosseron F. Feinstein D. L. Jacobs A. H. Wyss-Coray T. Vitorica J. Ransohoff R. M. Herrup K. Frautschy S. A. Finsen B. Brown G. C. Verkhratsky A. Yamanaka K. Koistinaho J. Latz E. Halle A. Petzold G. C. Town T. Morgan D. Shinohara M. L. Perry V. H. Holmes C. Bazan N. G. Brooks D. J. Hunot S. Joseph B. Deigendesch N. Garaschuk O. Boddeke E. Dinarello C. A. Breitner J. C. Cole G. M. Golenbock D. T. Kummer M. P. Lancet Neurol. 2015 14 388 405 25792098 PMC5909703
  22. O'Brien R. J. Wong P. C. Annu. Rev. Neurosci. 2011 34 185 204 21456963 PMC3174086
  23. Selkoe D. J. Neuron 1991 6 4 487 498 1673054
  24. Krabbe G. Halle A. Matyash V. Rinnenthal J. L. Eom G. D. Bernhardt U. Miller K. R. Prokop S. Kettenmann H. Heppner F. L. PLoS One 2013 8 e60921 23577177 PMC3620049
  25. Floden A. M. Combs C. K. J. Alzheimers. Dis. 2011 25 279 293 21403390 PMC3295838
  26. Sarlus H. Heneka M. T. J. Clin. Invest. 2017 127 3240 3249 28862638 PMC5669553
  27. Wes P. D. Holtman I. R. Boddeke E. W. G. M. Möller T. Eggen B. J. L. Glia 2016 64 197 213 26040959
  28. Jones R. S. Minogue A. M. Connor T. J. Lynch M. A. J. Neuroimmune Pharmacol. 2013 8 301 311 23238794
  29. Koenigsknecht J. Landreth G. J. Neurosci. 2004 24 9838 9846 15525768 PMC6730228
  30. Ribes S. Ebert S. Czesnik D. Regen T. Zeug A. Bukowski S. Mildner A. Eiffert H. Hanisch U.-K. Hammerschmidt S. Nau R. Infect. Immun. 2009 77 557 564 18981243 PMC2612236
  31. Hassan S. Eldeeb K. Millns P. J. Bennett A. J. Alexander S. P. H. Kendall D. A. Br. J. Pharmacol. 2014 171 2426 2439 24641282 PMC3997281
  32. Paresce D. M. Ghosh R. N. Maxfield F. R. Neuron 1996 17 553 565 8816718
  33. Lavis L. D. Rutkoski T. J. Raines R. T. Anal. Chem. 2007 79 6775 6782 17672523 PMC2868592
  34. Ma L. Ouyang Q. Werthmann G. C. Thompson H. M. Morrow E. M. Front. Cell Dev. Biol. 2017 5 71 28871281 PMC5566985
  35. Mauvezin C. Neufeld T. P. Autophagy 2015 11 1437 1438 26156798 PMC4590655
  36. Prakash P. Lantz T. C. Jethava K. P. Chopra G. Methods Protoc. 2019 2 48 PMC6632169 31181684
  37. Stine W. B. Jungbauer L. Yu C. Ladu M. J. Methods Mol. Biol. 2011 670 13 32 20967580 PMC3752843
  38. Stine W. B. Dahlgren K. N. Krafft G. A. LaDu M. J. J. Biol. Chem. 2003 278 11612 11622 12499373
  39. Bohlen C. J. Bennett F. C. Tucker A. F. Collins H. Y. Mulinyawe S. B. Barres B. A. Neuron 2017 94 759 773.e8 28521131 PMC5523817
  40. Liu S. Liu Y. Hao W. Wolf L. Kiliaan A. J. Penke B. Rube C. E. Walter J. Heneka M. T. Hartmann T. Menger M. D. Fassbender K. J. Immunol. 2012 188 1098 1107 22198949
  41. Liu Y. Walter S. Stagi M. Cherny D. Letiembre M. Schulz-Schaeffer W. Heine H. Penke B. Neumann H. Fassbender K. Brain 2005 128 1778 1789 15857927
  42. Zhao Y. Wu X. Li X. Jiang L.-L. Gui X. Liu Y. Sun Y. Zhu B. Piña-Crespo J. C. Zhang M. Zhang N. Chen X. Bu G. An Z. Huang T. Y. Xu H. Neuron 2018 97 1023 1031.e7 29518356 PMC5889092
  43. Parhizkar S. Arzberger T. Brendel M. Kleinberger G. Deussing M. Focke C. Nuscher B. Xiong M. Ghasemigharagoz A. Katzmarski N. Krasemann S. Lichtenthaler S. F. Müller S. A. Colombo A. Monasor L. S. Tahirovic S. Herms J. Willem M. Pettkus N. Butovsky O. Bartenstein P. Edbauer D. Rominger A. Ertürk A. Grathwohl S. A. Neher J. J. Holtzman D. M. Meyer-Luehmann M. Haass C. Nat. Neurosci. 2019 22 191 204 30617257 PMC6417433
  44. Kleinberger G. Yamanishi Y. Suarez-Calvet M. Czirr E. Lohmann E. Cuyvers E. Struyfs H. Pettkus N. Wenninger-Weinzierl A. Mazaheri F. Tahirovic S. Lleo A. Alcolea D. Fortea J. Willem M. Lammich S. Molinuevo J. L. Sanchez-Valle R. Antonell A. Ramirez A. Heneka M. T. Sleegers K. van der Zee J. Martin J.-J. Engelborghs S. Demirtas-Tatlidede A. Zetterberg H. Van Broeckhoven C. Gurvit H. Wyss-Coray T. Hardy J. Colonna M. Haass C. Sci. Transl. Med. 2014 6 243ra86 24990881
  45. Siddiqui T. A. Lively S. Vincent C. Schlichter L. C. J. Neuroinflammation 2012 9 770 PMC3551664 23158496
  46. Tasdemir-Yilmaz O. E. Freeman M. R. Genes Dev. 2014 28 20 33 24361692 PMC3894410
  47. Nuutila J. Lilius E.-M. Cytometry, Part A 2005 65 93 102 15825183
  48. Chow C.-W. Downey G. P. Grinstein S. Curr. Protoc. Cell Biol. 2004 22 15.7.1 15.7.33 18228442
  49. Butovsky O. Jedrychowski M. P. Moore C. S. Cialic R. Lanser A. J. Gabriely G. Koeglsperger T. Dake B. Wu P. M. Doykan C. E. Fanek Z. Liu L. Chen Z. Rothstein J. D. Ransohoff R. M. Gygi S. P. Antel J. P. Weiner H. L. Nat. Neurosci. 2014 17 131 143 24316888 PMC4066672
  50. Xiao Q. Yan P. Ma X. Liu H. Perez R. Zhu A. Gonzales E. Burchett J. M. Schuler D. R. Cirrito J. R. Diwan A. Lee J.-M. J. Neurosci. 2014 34 9607 9620 25031402 PMC4099542
  51. Fernández-Albarral J. A. Salobrar-García E. Martínez-Páramo R. Ramírez A. I. de Hoz R. Ramírez J. M. Salazar J. J. J. Optom. 2019 12 198 207 30377086 PMC6612028
  52. Gomez-Arboledas A. Davila J. C. Sanchez-Mejias E. Navarro V. Nuñez-Diaz C. Sanchez-Varo R. Sanchez-Mico M. V. Trujillo-Estrada L. Fernandez-Valenzuela J. J. Vizuete M. Comella J. X. Galea E. Vitorica J. Gutierrez A. Glia 2018 66 637 653 29178139 PMC5814816
  53. Ruan C. Sun L. Kroshilina A. Beckers L. De Jager P. Bradshaw E. M. Hasson S. A. Yang G. Elyaman W. Brain. Behav. Immun. 2020 83 180 191 31604143
  54. Streit W. J. Sammons N. W. Kuhns A. J. Sparks D. L. Glia 2004 45 208 212 14730714
  55. Streit W. J. Braak H. Xue Q. S. Bechmann I. Acta Neuropathol 2009 118 475 485 19513731 PMC2737117
  56. Guneykaya D. Ivanov A. Hernandez D. P. Haage V. Wojtas B. Meyer N. Maricos M. Jordan P. Buonfiglioli A. Gielniewski B. Ochocka N. Cömert C. Friedrich C. Artiles L. S. Kaminska B. Mertins P. Beule D. Kettenmann H. Wolf S. A. Cell Rep 2018 24 2773 2783.e6 30184509
  57. Mildner A. Schlevogt B. Kierdorf K. Böttcher C. Erny D. Kummer M. P. Quinn M. Brück W. Bechmann I. Heneka M. T. Priller J. Prinz M. J. Neurosci. 2011 31 11159 11171 21813677 PMC6623351
  58. Grathwohl S. A. Kälin R. E. Bolmont T. Prokop S. Winkelmann G. Kaeser S. A. Odenthal J. Radde R. Eldh T. Gandy S. Aguzzi A. Staufenbiel M. Mathews P. M. Wolburg H. Heppner F. L. Jucker M. Nat. Neurosci. 2009 12 1361 1363 19838177 PMC4721582
  59. Foo L. C. Allen N. J. Bushong E. A. Ventura P. B. Chung W.-S. Zhou L. Cahoy J. D. Daneman R. Zong H. Ellisman M. H. Barres B. A. Neuron 2011 71 799 811 21903074 PMC3172573

Authors
Prakash, P., Jethava, K. P., Korte, N., Izquierdo, P., Favuzzi, E., Rose, I. V. L., Guttenplan, K. A., Manchanda, P., Dutta, S., Rochet, J.-C., Fishell, G., Liddelow, S. A., Attwell, D., & Chopra, G.
Affiliations
  • Purdue University West Lafayette (US)
  • University College London (GB)
  • Harvard University (US)
  • New York University (US)
  • Stanford Medicine (US)
Corresponding author
Chopra, Gaurav
ORCID
Venue
Chemical Science
Publisher
Royal Society of Chemistry (RSC)
Published
2021
Volume
12
Issue
32
Pages
10901-10918
ISSN
  • 2041-6520 (ISSN-L)
  • 2041-6539
DOI
10.1039/d1sc03486c
PMC
PMC8372545
OpenAlex
W3184506354
Cited by
45
References
62
Citations by year
2026: 5 · 2025: 13 · 2024: 5 · 2023: 14 · 2022: 5 · 2021: 3
FWCI
4.24
Type
journal article
Language
en
Open access
Licence
Locations
  • Chemical Science (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Minerva Access (University of Melbourne) (submittedVersion, cc-by)
Funders
  • Purdue University
  • Purdue Research Foundation
  • Ralph W. and Grace M. Showalter Research Trust Fund
  • National Institutes of Health (P30 CA023168, R03NS108229)
  • National Center for Advancing Translational Sciences
  • U.S. Department of Defense (W81XWH2010665)
  • Indiana Alzheimer Disease Center, School of Medicine, Indiana University
  • Eli Lilly and Company
  • Indiana Clinical and Translational Sciences Institute (UL1TR002529)
  • Wellcome Trust (099222, 219366)
  • Biotechnology and Biological Sciences Research Council
  • European Molecular Biology Organization (ALTF 444-2018)
  • Stanford University
  • School of Medicine, New York University
  • Blas Frangione Foundation
  • Cure Alzheimer's Fund
Topics
  • Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
  • Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
  • Health Sciences › Medicine › Epidemiology › Autophagy in Disease and Therapy
Keywords
amyloid-beta, phagocytosis, glial cells, lysosomes, real-time imaging, Alzheimer's disease, amyloid-beta clearance

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review