Process Integrated Biosensors for Real‐Time Monitoring of Antibodies for Automated Affinity Purification
Abstract
A sensor technology for in-line detection of monoclonal antibodies (mAbs) in affinity capture steps is demonstrated. Continuous mAb monitoring during multiple consecutive purification cycles can facilitate automatization and continuous processing.
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LSPR biosensors have been utilized for in-line monitoring of the Protein A capture step, enabling high-sensitivity detection of column breakthrough by measuring product concentrations as low as 2 μg/mL in the effluent.
“LSPR biosensors have been utilized for in‐line monitoring of the Protein A capture step, enabling the high‐sensitivity detection of column breakthrough by measuring product concentrations as low as 2 µg/mL in the effluent, allowing for early identification of column leakage [96].”
From Current PAT Landscape in the Downstream Processing of Biopharmaceuticals (2025) · cites this paper as [96]
LSPR biosensing detects biomolecular interactions by monitoring shifts in the LSPR band.
“LSPR biosensing detects biomolecular interactions by monitoring shifts in this band.”
From Current PAT Landscape in the Downstream Processing of Biopharmaceuticals (2025) · cites this paper as [96]
LSPR biosensors are less sensitive to changes in temperature, sample composition and vibrations, and unlike spectroscopic techniques, they do not require advanced data analysis to extract product concentrations.
“LSPR biosensors are less sensitive to changes in temperature, sample composition and vibrations, and unlike spectroscopic techniques, they do not require advanced data analysis to extract product concentrations [96].”
From Current PAT Landscape in the Downstream Processing of Biopharmaceuticals (2025) · cites this paper as [96]
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Research works printed but not linked: 26
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Current PAT Landscape in the Downstream Processing of Biopharmaceuticals
Sathiyapriyan, P., Mukherjee, S., Vogel, T., Essen, L., Boerema, D., Vey, M., & Kalina, U. (2025) · Analytical Science Advances · doi:10.1002/ansa.70013 · Cited by 45 · see citing works
- Authors
- Tran, T., Martinsson, E., Gustavsson, R., Tronarp, O., Nilsson, M., Hansson, K. R., Lundström, I., Mandenius, C.-F., & Aili, D.
- Affiliations
- Laboratory of Molecular Materials, Division of Biophysics and Bioengineering, Department of Physics, Chemistry and Biology, Linköping University, Linköping 581 83, Sweden
- ArgusEye AB, Spannmålsgatan 55, Linköping 583 36, Sweden
- Biotechnology, Division of Biophysics and Bioengineering, Department of Physics, Chemistry and Biology, Linköping University, Linköping 581 83, Sweden
- Wolfram MathCore AB, Teknikringen 1E, Linköping 583 30, Sweden
- BioInvent International AB, Ideon Science Park, Lund 223 70, Sweden
- Sensor and Actuator Systems, Department of Physics, Chemistry and Biology, Linköping University, Linköping 581 83, Sweden
- ORCID
- Aili, Daniel
- Venue
- Analytical Methods
- Publisher
- Royal Society of Chemistry (RSC)
- Published
- 2022
- Volume
- 14
- Issue
- 44
- Pages
- 4555-4562
- ISSN
- 1759-9660
- 1759-9679
- Cited by
- 10
- References
- 0
- Language
- en
- Funders
- H2020 Marie Skłodowska-Curie Actions (841373)
- VINNOVA (2016-04120, 2019-00130)
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