Development of substrates for the culture of human pluripotent stem cells
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This review provides the latest progress of culture substrates for human pluripotent stem cells.
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Single glycoproteins such as laminin, fibronectin, and vitronectin promote integrin-mediated adhesion.
“including the use of single glycoproteins such as laminin, fibronectin, and vitronectin, which promote integrin-mediated adhesion (Hayashi and Furue, 2016; Aisenbrey and Murphy, 2020; Kawase and Nakatsuji, 2023).”
From Challenges and solutions in transitioning to animal-free standards: a comprehensive analysis of components in human cell-based developmental neurotoxicity assays (2026) · cites this paper as Kawase and Nakatsuji, 2023
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Feeder-independent culture of human embryonic stem cells
Ludwig, T. E., Bergendahl, V., Levenstein, M. E., Yu, J., Probasco, M. D., & Thomson, J. A. (2006) · Nature Methods · doi:10.1038/nmeth902 · Cited by 681 · see citing works
Matrigel: From discovery and ECM mimicry to assays and models for cancer research
Benton, G., Arnaoutova, I., George, J., Kleinman, H. K., & Koblinski, J. (2014) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2014.06.005 · Cited by 477 · see citing works
Miyazaki, T., Futaki, S., Suemori, H., Taniguchi, Y., Yamada, M., Kawasaki, M., Hayashi, M., Kumagai, H., Nakatsuji, N., Sekiguchi, K., et al. (2012) · Nature Communications · doi:10.1038/ncomms2231 · Cited by 368 · see citing works
Self-citationScalable Stirred-Suspension Bioreactor Culture of Human Pluripotent Stem Cells
Kehoe, D. E., Jing, D., Lock, L. T., & Tzanakakis, E. S. (2010) · Tissue Engineering Part A · doi:10.1089/ten.tea.2009.0454 · Cited by 264 · see citing works
Thermoreversible Hyaluronic Acid‐PNIPAAm Hydrogel Systems for 3D Stem Cell Culture
Ekerdt, B. L., Fuentes, C. M., Lei, Y., Adil, M. M., Ramasubramanian, A., Segalman, R. A., & Schaffer, D. V. (2018) · Advanced Healthcare Materials · doi:10.1002/adhm.201800225 · Cited by 117 · see citing works
Abecasis, B., Aguiar, T., Arnault, É., Costa, R., Gomes-Alves, P., Aspegren, A., Serra, M., & Alves, P. M. (2017) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2017.01.004 · Cited by 100 · see citing works
Etezadi, F., Le, M. N. T., Shahsavarani, H., Alipour, A., Moazzezy, N., Samani, S., Amanzadeh, A., Pahlavan, S., Bonakdar, S., Shokrgozar, M. A., et al. (2022) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.2c00162 · Cited by 33 · see citing works
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Research works printed but not linked: 112
- M. J. Evans and M. H. Kaufman, Establishment in culture of pluripotential cells from mouse embryos, Nature, 1981, 292, 154-156.
- G. R. Martin, Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by tera- tocarcinoma stem cells, Proc. Natl. Acad. Sci. U. S. A., 1981, 78, 7634-7638.
- J. A. Thomson, J. Itskovitz-Eldor, S. S. Shapiro, M. A. Waknitz, J. J. Swiergiel, V. S. Marshall and J. M. Jones, Embryonic stem cell lines derived from human blastocysts, Science, 1988, 282, 1145-1147.
- A. G. Smith, J. K. Heath, D. D. Donaldson, G. G. Wong, J. Moreau, M. Stahl and D. Rogers, Inhibition of pluripo- tential embryonic stem cell differentiation by purified polypeptides, Nature, 1988, 336, 688-690.
- K. Takahashi and S. Yamanaka, Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cul- tures by defined factors, Cell, 2006, 126, 663-676.
- K. Takahashi, K. Tanabe, M. Ohnuki, M. Narita, T. Ichisaka, K. Tomoda and S. Yamanaka, Induction of pluripotent stem cells from adult human fibroblasts by defined factors, Cell, 2007, 131, 861-872.
- J. Yu, M. A. Vodyanik, K. Smuga-Otto, J. Antosiewicz- Bourget, J. L. Frane, S. Tian, J. Nie, G. A. Jonsdottir, V. Ruotti, R. Stewart, I. I. Slukvin and J. A. Thomson, Induced pluripotent stem cell lines derived from human somatic cells, Science, 2007, 318, 1917-1920.
- A. Higuchi, Q. D. Ling, Y. A. Ko, Y. Chang and A. Umezawa, Biomaterials for the feeder-free culture of human embryonic stem cells and induced pluripotent stem cells, Chem. Rev., 2011, 111, 3021-3035.
- A. Higuchi, Q. D. Ling, S. Kumar, M. Munusamy, A. A. Alarfajj, A. Umezawa and G. J. Wu, Design of poly- meric materials for culturing human pluripotent stem cells: Progress toward feeder-free and xeno-free culturing, Prog. Polym. Sci., 2014, 39, 1348-1374.
- S. Schmidt, A. Lilienkampf and M. Bradley, New sub- strates for stem cell control, Philos. Trans. R. Soc., B, 2018, 373, 20170223.
- A. Polanco, B. Kuang and S. Yoon, Bioprocess Technologies that Preserve the Quality of iPSCs, Trends Biotechnol., 2020, 38, 1128-1140.
- S. E. Tannenbaum and B. E. Reubinoff, Advances in hPSC expansion towards therapeutic entities: A review, Cell Proliferation, 2022, 55, e13247.
- C. Xu, M. S. Inokuma, J. Denham, K. Golds, P. Kundu, J. D. Gold and M. K. Carpenter, Feeder-free growth of undifferentiated human embryonic stem cells, Nat. Biotechnol., 2001, 19, 971-974.
- M. Amit, C. Shariki, V. Margulets and J. Itskovitz-Eldor, Feeder layer-and serum-free culture of human embryonic stem cells, Biol. Reprod., 2004, 70, 837-845.
- D. James, A. J. Levine, D. Besser and A. Hemmati- Brivanlou, TGFbeta/activin/nodal signaling is necessary for the maintenance of pluripotency in human embryonic stem cells, Development, 2005, 132, 1273-1282.
- I. Klimanskaya, Y. Chung, L. Meisner, J. Johnson, M. D. West and R. Lanza, Human embryonic stem cells derived without feeder cells, Lancet, 2005, 365, 1636-1641.
- G. Wang, H. Zhang, Y. Zhao, J. Li, J. Cai, P. Wang, S. Meng, J. Feng, C. Miao, M. Ding, D. Li and H. Deng, Noggin and bFGF cooperate to maintain the pluripotency of human embryonic stem cells in the absence of feeder layers, Biochem. Biophys. Res. Commun., 2005, 330, 934- 942.
- R. H. Xu, R. M. Peck, D. S. Li, X. Feng, T. Ludwig and J. A. Thomson, Basic FGF and suppression of BMP signal- ing sustain undifferentiated proliferation of human ES cells, Nat. Methods, 2005, 2, 185-190.
- M. K. Furue, J. Na, J. P. Jackson, T. Okamoto, M. Jones, D. Baker, R. Hata, H. D. Moore, J. D. Sato and P. W. Andrews, Heparin promotes the growth of human embryonic stem cells in a defined serum-free medium, Proc. Natl. Acad. Sci. U. S. A., 2008, 105, 13409-13414.
- Y. Li, S. Powell, E. Brunette, J. Lebkowski and R. Mandalam, Expansion of human embryonic stem cells in defined serum-free medium devoid of animal-derived products, Biotechnol. Bioeng., 2005, 91, 688-698.
- Y. Liu, Z. Song, Y. Zhao, H. Qin, J. Cai, H. Zhang, T. Yu, S. Jiang, G. Wang, M. Ding and H. Deng, A novel chemi- cal-defined medium with bFGF and N2B27 supplements supports undifferentiated growth in human embryonic stem cells, Biochem. Biophys. Res. Commun., 2006, 346, 131-139.
- J. Lu, R. Hou, C. J. Booth, S. H. Yang and M. Snyder, Defined culture conditions of human embryonic stem cells, Proc. Natl. Acad. Sci. U. S. A., 2006, 103, 5688-5693.
- L. Vallier, M. Alexander and R. A. Pedersen, Activin/Nodal and FGF pathways cooperate to maintain pluripotency of human embryonic stem cells, J. Cell Sci., 2005, 118, 4495- 4509.
- S. Yao, S. Chen, J. Clark, E. Hao, G. M. Beattie, A. Hayek and S. Ding, Long-term self-renewal and directed differen- tiation of human embryonic stem cells in chemically defined conditions, Proc. Natl. Acad. Sci. U. S. A., 2006, 103, 6907-6912.
- L. Wang, T. C. Schuiz, E. S. Sherrer, D. S. Dauphin, S. Shin, A. M. Nelson, C. B. Ware, M. Zhan, C. Z. Song, X. Chen, S. N. Brimble, A. McLean, M. J. Galeano, E. W. Uhl, K. A. D'Amour, J. D. Chesnut, M. S. Rao, C. A. Blau and A. J. Robins, Self-renewal of human embryonic stem cells requires insulin-like growth factor-1 receptor and ERBB2 receptor signaling, Blood, 2007, 110, 4111-4119.
- T. E. Ludwig, M. E. Levenstein, J. M. Jones, W. T. Berggren, E. R. Mitchen, J. L. Frane, L. J. Crandall, C. A. Daigh, K. R. Conard, M. S. Piekarczyk, R. A. Llanas and J. A. Thomson, Derivation of human embryonic stem cells in defined conditions, Nat. Biotechnol., 2006, 24, 185-187.
- International Stem Cell Initiative Consortium; V. Akopian, P. W. Andrews, S. Beil, N. Benvenisty, J. Brehm, M. Christie, A. Ford, V. Fox, P. J. Gokhale, L. Healy, F. Holm, O. Hovatta, B. B. Knowles, T. E. Ludwig, R. D. McKay, T. Miyazaki, N. Nakatsuji, S. K. Oh, M. F. Pera, J. Rossant, G. N. Stacey and H. Suemori, Comparison of defined culture systems for feeder cell free propagation of human embryonic stem cells, In Vitro Cell. Dev. Biol.: Anim., 2010, 46, 247-258.
- G. Chen, D. R. Gulbranson, Z. Hou, J. M. Bolin, V. Ruotti, M. D. Probasco, K. Smuga-Otto, S. E. Howden, N. R. Diol, N. E. Propson, R. Wagner, G. O. Lee, J. Antosiewicz- Bourget, J. M. Teng and J. A. Thomson, Chemically defined conditions for human iPSC derivation and culture, Nat. Methods, 2011, 8, 424-429.
- K. Watanabe, M. Ueno, D. Kamiya, A. Nishiyama, M. Matsumura, T. Wataya, J. B. Takahashi, S. Nishikawa, K. Muguruma and Y. Sasai, A ROCK inhibitor permits sur- vival of dissociated human embryonic stem cells, Nat. Biotechnol., 2007, 25, 681-686.
- S. I. Vernardis, K. Terzoudis, N. Panoskaltsis and A. Mantalaris, Human embryonic and induced pluripo- tent stem cells maintain phenotype but alter their metab- olism after exposure to ROCK inhibitor, Sci. Rep., 2017, 7, 42138.
- S. Kaur, I. Kaur, P. Rawal, D. M. Tripathi and A. Vasudevan, Non-matrigel scaffolds for organoid cul- tures, Cancer Lett., 2021, 504, 58-66.
- M. T. Kozlowski, C. J. Crook and H. T. Ku, Towards organoid culture without Matrigel, Commun. Biol., 2021, 4, 1387.
- H. K. Kleinman, M. L. McGarvey, J. R. Hassell, V. L. Star, F. B. Cannon, G. W. Laurie and G. R. Martin, Basement membrane complexes with biological activity, Biochemistry, 1986, 25, 312-318. Corning® Matrigel® Matrix Frequently Asked Questions.
- C. S. Hughes, L. M. Postovit and G. A. Lajoie, Matrigel: a complex protein mixture required for optimal growth of cell culture, Proteomics, 2010, 10, 1886-1890.
- N. C. Talbot and T. J. Caperna, Proteome array identifi- cation of bioactive soluble proteins/peptides in Matrigel: relevance to stem cell responses, Cytotechnology, 2015, 67, 873-883.
- S. Vukicevic, H. K. Kleinman, F. P. Luyten, A. B. Roberts, N. S. Roche and A. H. Reddi, Identification of multiple active growth factors in basement membrane Matrigel suggests caution in interpretation of cellular activity related to extracellular matrix components, Exp. Cell Res., 1992, 202, 1-8.
- J. A. Carlson Scholz, R. Garg, S. R. Compton, H. G. Allore, C. J. Zeiss and E. M. Uchio, Poliomyelitis in MuLV- infected ICR-SCID mice after injection of basement mem- brane matrix contaminated with lactate dehydrogenase- elevating virus, Comp. Med., 2011, 61, 404-411.
- M. J. Martin, A. Muotri, F. Gage and A. Varki, Human embryonic stem cells express an immunogenic nonhu- man sialic acid, Nat. Med., 2005, 11, 228-232.
- R. O. Hynes, Integrins: bidirectional, allosteric signaling machines, Cell, 2002, 110, 673-687.
- T. Miyazaki, S. Futaki, K. Hasegawa, M. Kawasaki, N. Sanzen, M. Hayashi, E. Kawase, K. Sekiguchi, N. Nakatsuji and H. Suemori, Recombinant human laminin isoforms can support the undifferentiated growth of human embryonic stem cells, Biochem. Biophys. Res. Commun., 2008, 375, 27-32.
- L. G. Villa-Diaz, J. K. Kim, A. Laperle, S. P. Palecek and P. H. Krebsbach, Inhibition of Focal Adhesion Kinase Signaling by Integrin α6β1 Supports Human Pluripotent Stem Cell Self-Renewal, Stem Cells, 2016, 34, 1753-1764.
- R. Nishiuchi, J. Takagi, M. Hayashi, H. Ido, Y. Yagi, N. Sanzen, T. Tsuji, M. Yamada and K. Sekiguchi, Ligand- binding specificities of laminin-binding integrins: a com- prehensive survey of laminin-integrin interactions using recombinant α3β1, α6β1, α7β1 and α6β4 integrins, Matrix Biol., 2006, 25, 189-197.
- J. H. Miner and P. D. Yurchenco, Laminin functions in tissue morphogenesis, Annu. Rev. Cell Dev. Biol., 2004, 20, 255-284.
- S. Rodin, A. Domogatskaya, S. Ström, E. M. Hansson, K. R. Chien, J. Inzunza, O. Hovatta and K. Tryggvason, Long-term self-renewal of human pluripotent stem cells on human recombinant laminin-511, Nat. Biotechnol., 2010, 28, 611-615.
- S. Rodin, L. Antonsson, C. Niaudet, O. E. Simonson, E. Salmela, E. M. Hansson, A. Domogatskaya, Z. Xiao, P. Damdimopoulou, M. Sheikhi, J. Inzunza, A. S. Nilsson, D. Baker, R. Kuiper, Y. Sun, E. Blennow, M. Nordenskjöld, K. H. Grinnemo, J. Kere, C. Betsholtz, O. Hovatta and K. Tryggvason, Clonal culturing of human embryonic stem cells on laminin-521/E-cadherin matrix in defined and xeno-free environment, Nat. Commun., 2014, 5, 3195.
- H. Ido, A. Nakamura, R. Kobayashi, S. Ito, S. Li, S. Futaki and K. Sekiguchi, The requirement of the glutamic acid residue at the third position from the carboxyl termini of the laminin gamma chains in integrin binding by lami- nins, J. Biol. Chem., 2007, 282, 11144-11154.
- T. Miyazaki, T. Isobe, N. Nakatsuji and H. Suemori, Efficient Adhesion Culture of Human Pluripotent Stem Cells Using Laminin Fragments in an Uncoated Manner, Sci. Rep., 2017, 7, 41165.
- H. Main, M. Hedenskog, G. Acharya, O. Hovatta and F. Lanner, Karolinska Institutet Human Embryonic Stem Cell Bank, Stem Cell Res., 2020, 45, 101810.
- E. Kawase, K. Takada, R. Nakatani, S. Yamazaki and H. Suemori, Generation of clinical-grade human embryo- nic stem cell line KthES11 according to Japanese regu- lations, Stem Cell Res., 2021, 54, 102383.
- T. Souralova, D. Rehakova, M. Jeseta, L. Tesarova, J. Beranek, P. Ventruba, A. Hampl and I. Koutna, The Manufacture of Xeno-and Feeder-Free Clinical-Grade Human Embryonic Stem Cell Lines: First Step for Cell Therapy, Int. J. Mol. Sci., 2022, 23, 12500.
- K. Takada, R. Nakatani, E. Moribe, S. Yamazaki-Fujigaki, M. Fujii, M. Furuta, H. Suemori and E. Kawase, Efficient derivation and banking of clinical-grade human embryo- nic stem cell lines in accordance with Japanese regu- lations, Regen. Ther., 2022, 21, 553-559.
- I. Schvartz, D. Seger and S. Shaltiel, Vitronectin, Int. J. Biochem. Cell Biol., 1999, 31, 539-544.
- S. R. Braam, L. Zeinstra, S. Litjens, D. Ward-van Oostwaard, S. van den Brink, L. van Laake, F. Lebrin, P. Kats, R. Hochstenbach, R. Passier, A. Sonnenberg and C. L. Mummery, Recombinant vitronectin is a functionally defined substrate that supports human embryonic stem cell self-renewal via alphavbeta5 integrin, Stem Cells, 2008, 26, 2257-2265.
- M. Takeichi, Morphogenetic roles of classic cadherins, Curr. Opin. Cell Biol., 1995, 7, 619-627.
- A. M. Eastham, H. Spencer, F. Soncin, S. Ritson, C. L. Merry, P. L. Stern and C. M. Ward, Epithelial-mesenchymal tran- sition events during human embryonic stem cell differen- tiation, Cancer Res., 2007, 67, 11254-11262.
- D. Li, J. Zhou, L. Wang, M. E. Shin, P. Su, X. Lei, H. Kuang, W. Guo, H. Yang, L. Cheng, T. S. Tanaka, D. E. Leckband, A. B. Reynolds, E. Duan and F. Wang, Integrated biochemical and mechanical signals regulate multifaceted human embryonic stem cell functions, J. Cell Biol., 2010, 191, 631-644.
- M. Nagaoka, K. Si-Tayeb, T. Akaike and S. A. Duncan, Culture of human pluripotent stem cells using completely defined conditions on a recombinant E-cadherin substra- tum, BMC Dev. Biol., 2010, 10, 60.
- Z. Melkoumian, J. L. Weber, D. M. Weber, A. G. Fadeev, Y. Zhou, P. Dolley-Sonneville, J. Yang, L. Qiu, C. A. Priest, C. Shogbon, A. W. Martin, J. Nelson, P. West, J. P. Beltzer, S. Pal and R. Brandenberger, Synthetic peptide-acrylate surfaces for long-term self-renewal and cardiomyocyte differentiation of human embryonic stem cells, Nat. Biotechnol., 2010, 28, 606-610.
- S. Jin, H. Yao, J. L. Weber, Z. K. Melkoumian and K. Ye, A synthetic, xeno-free peptide surface for expansion and directed differentiation of human induced pluripotent stem cells, PLoS One, 2012, 7, e50880.
- E. Kawase, Efficient Expansion of Dissociated Human Pluripotent Stem Cells Using a Synthetic Substrate, Methods Mol. Biol., 2016, 1307, 61-69.
- B. A. Tucker, K. R. Anfinson, R. F. Mullins, E. M. Stone and M. J. Young, Use of a synthetic xeno-free culture sub- strate for induced pluripotent stem cell induction and retinal differentiation, Stem Cells Transl. Med., 2013, 2, 16-24.
- Y. Li, A. Gautam, J. Yang, L. Qiu, Z. Melkoumian, J. Weber, L. Telukuntla, R. Srivastava, E. M. Whiteley and R. Brandenberger, Differentiation of oligodendrocyte pro- genitor cells from human embryonic stem cells on vitro- nectin-derived synthetic peptide acrylate surface, Stem Cells Dev., 2013, 22, 1497-1505.
- P. Y. Lin, S. H. Hung, Y. C. Yang, L. C. Liao, Y. C. Hsieh, H. J. Yen, H. E. Lu, M. S. Lee, I. M. Chu and S. M. Hwang, A synthetic peptide-acrylate surface for production of insulin-producing cells from human embryonic stem cells, Stem Cells Dev., 2014, 23, 372-379.
- A. Higuchi, S. H. Kao, Q. D. Ling, Y. M. Chen, H. F. Li, A. A. Alarfaj, M. A. Munusamy, K. Murugan, S. C. Chang, H. C. Lee, S. T. Hsu, S. S. Kumar and A. Umezawa, Long- term xeno-free culture of human pluripotent stem cells on hydrogels with optimal elasticity, Sci. Rep., 2015, 5, 18136.
- Y. M. Chen, L. H. Chen, M. P. Li, H. F. Li, A. Higuchi, S. S. Kumar, Q. D. Ling, A. A. Alarfaj, M. A. Munusamy, Y. Chang, G. Benelli, K. Murugan and A. Umezawa, Xeno- free culture of human pluripotent stem cells on oligopep- tide-grafted hydrogels with various molecular designs, Sci. Rep., 2017, 7, 45146.
- J. R. Klim, L. Li, P. J. Wrighton, M. S. Piekarczyk and L. L. Kiessling, A defined glycosaminoglycan-binding sub- stratum for human pluripotent stem cells, Nat. Methods, 2010, 7, 989-994.
- Y. Deng, X. Zhang, X. Zhao, Q. Li, Z. Ye, Z. Li, Y. Liu, Y. Zhou, H. Ma, G. Pan, D. Pei, J. Fang and S. Wei, Long- term self-renewal of human pluripotent stem cells on peptide-decorated poly(OEGMA-co-HEMA) brushes under fully defined conditions, Acta Biomater., 2013, 9, 8840- 8850.
- H. J. Park, K. Yang, M. J. Kim, J. Jang, M. Lee, D. W. Kim, H. Lee and S. W. Cho, Bio-inspired oligovitronectin- grafted surface for enhanced self-renewal and long-term maintenance of human pluripotent stem cells under feeder-free conditions, Biomaterials, 2015, 50, 127-139.
- P. Zhou, F. Wu, T. Zhou, X. Cai, S. Zhang, X. Zhang, Q. Li, Y. Li, Y. Zheng, M. Wang, F. Lan, G. Pan, D. Pei and S. Wei, Simple and versatile synthetic polydopamine- based surface supports reprogramming of human somatic cells and long-term self-renewal of human pluripotent stem cells under defined conditions, Biomaterials, 2016, 87, 1-17.
- P. Zhou, B. Yin, R. Zhang, Z. Xu, Y. Liu, Y. Yan, X. Zhang, S. Zhang, Y. Li, H. Liu, Y. A. Yuan and S. Wei, Molecular basis for RGD-containing peptides supporting adhesion and self-renewal of human pluripotent stem cells on syn- thetic surface, Colloids Surf., B, 2018, 171, 451-460.
- J. W. Lambshead, L. Meagher, J. Goodwin, T. Labonne, E. Ng, A. Elefanty, E. Stanley, C. M. O'Brien and A. L. Laslett, Long-Term Maintenance of Human Pluripotent Stem Cells on cRGDfK-Presenting Synthetic Surfaces, Sci. Rep., 2018, 8, 701.
- A. A. Alarfaj, A. H. Hirad, M. A. Munusamy, S. S. Kumar and A. Higuchi, Human embryonic stem cells cultured on hydrogels grafted with extracellular matrix protein-derived peptides with polyethylene glycol joint nanosegments, IET Nanobiotechnol., 2022, 16, 295-304.
- T. C. Sung, M. W. Lu, Z. Tian, H. H. Lee, J. Pan, Q. D. Ling and A. Higuchi, Poly(vinyl alcohol-co-itaconic acid) hydro- gels grafted with several designed peptides for human pluripotent stem cell culture and differentiation into car- diomyocytes, J. Mater. Chem. B, 2021, 9, 7662-7673.
- L. G. Villa-Diaz, H. Nandivada, J. Ding, N. C. Nogueira-de- Souza, P. H. Krebsbach, K. S. O'Shea, J. Lahann and G. D. Smith, Synthetic polymer coatings for long-term growth of human embryonic stem cells, Nat. Biotechnol., 2010, 28, 581-583.
- Y. Mei, K. Saha, S. R. Bogatyrev, J. Yang, A. L. Hook, Z. I. Kalcioglu, S. W. Cho, M. Mitalipova, N. Pyzocha, F. Rojas, K. J. Van Vliet, M. C. Davies, M. R. Alexander, R. Langer, R. Jaenisch and D. G. Anderson, Combinatorial development of biomaterials for clonal growth of human pluripotent stem cells, Nat. Mater., 2010, 9, 768-778.
- D. A. Brafman, C. W. Chang, A. Fernandez, K. Willert, S. Varghese and S. Chien, Long-term human pluripotent stem cell self-renewal on synthetic polymer surfaces, Biomaterials, 2010, 31, 9135-9144.
- E. F. Irwin, R. Gupta, D. C. Dashti and K. E. Healy, Engineered polymer-media interfaces for the long-term self-renewal of human embryonic stem cells, Biomaterials, 2011, 32, 6912-6919.
- C. W. Chang, Y. Hwang, D. Brafman, T. Hagan, C. Phung and S. Varghese, Engineering cell-material interfaces for long-term expansion of human pluripotent stem cells, Biomaterials, 2013, 34, 912-921.
- N. T. Kohen, L. E. Little and K. E. Healy, Characterization of Matrigel interfaces during defined human embryonic stem cell culture, Biointerphases, 2009, 4, 69-79.
- S. Musah, S. A. Morin, P. J. Wrighton, D. B. Zwick, S. Jin and L. L. Kiessling, Glycosaminoglycan-binding hydrogels enable mechanical control of human pluripotent stem cell self-renewal, ACS Nano, 2012, 6, 10168-10177.
- S. Paiva, P. Joanne, C. Migdal, E. L. Soler, Y. Hovhannisyan, A. Nicolas and O. Agbulut, Polyacrylamide Hydrogels with Rigidity-Independent Surface Chemistry Show Limited Long- Term Maintenance of Pluripotency of Human Induced Pluripotent Stem Cells on Soft Substrates, ACS Biomater. Sci. Eng., 2020, 6, 340-351.
- M. Serra, C. Brito, C. Correia and P. M. Alves, Process engineering of human pluripotent stem cells for clinical application, Trends Biotechnol., 2012, 30, 350-359.
- S. Tohyama, J. Fujita, C. Fujita, M. Yamaguchi, S. Kanaami, R. Ohno, K. Sakamoto, M. Kodama, J. Kurokawa, H. Kanazawa, T. Seki, Y. Kishino, M. Okada, K. Nakajima, S. Tanosaki, S. Someya, A. Hirano, S. Kawaguchi, E. Kobayashi and K. Fukuda, Efficient Large-Scale 2D Culture System for Human Induced Pluripotent Stem Cells and Differentiated Cardiomyocytes, Stem Cell Rep., 2017, 9, 1406-1414.
- A. T. Lam, J. Li, A. K. Chen, W. R. Birch, S. Reuveny and S. K. Oh, Improved Human Pluripotent Stem Cell Attachment and Spreading on Xeno-Free Laminin-521- Coated Microcarriers Results in Efficient Growth in Agitated Cultures, BioRes. Open Access, 2015, 4, 242-257.
- S. M. Badenes, T. G. Fernandes, C. S. Cordeiro, S. Boucher, D. Kuninger, M. C. Vemuri, M. M. Diogo and J. M. Cabral, Defined Essential 8™ Medium and Vitronectin Efficiently Support Scalable Xeno-Free Expansion of Human Induced Pluripotent Stem Cells in Stirred Microcarrier Culture Systems, PLoS One, 2016, 11, e0151264.
- Y. Fan, F. Zhang and E. S. Tzanakakis, Engineering Xeno- Free Microcarriers with Recombinant Vitronectin, Albumin and UV Irradiation for Human Pluripotent Stem Cell Bioprocessing, ACS Biomater. Sci. Eng., 2017, 3, 1510- 1518.
- Y. Fan, M. Hsiung, C. Cheng and E. S. Tzanakakis, Facile engineering of xeno-free microcarriers for the scalable cul- tivation of human pluripotent stem cells in stirred sus- pension, Tissue Eng., Part A, 2014, 20, 588-599.
- A. L. Rodrigues, C. A. V. Rodrigues, A. R. Gomes, S. F. Vieira, S. M. Badenes, M. M. Diogo and J. M. S. Cabral, Dissolvable Microcarriers Allow Scalable Expansion And Harvesting Of Human Induced Pluripotent Stem Cells Under Xeno-Free Conditions, Biotechnol. J., 2019, 14, e1800461.
- S. Derakhti, S. H. Safiabadi-Tali, G. Amoabediny and M. Sheikhpour, Attachment and detachment strategies in microcarrier-based cell culture technology: A comprehen- sive review, Mater. Sci. Eng., C, 2019, 103, 109782.
- P. Fattahi, A. Rahimian, M. Q. Slama, K. Gwon, A. M. Gonzalez-Suarez, J. Wolf, H. Baskaran, C. D. Duffy, G. Stybayeva, Q. P. Peterson and A. Revzin, Core-shell hydrogel microcapsules enable formation of human pluri- potent stem cell spheroids and their cultivation in a stirred bioreactor, Sci. Rep., 2021, 11, 7177.
- V. C. Chen, S. M. Couture, J. Ye, Z. Lin, G. Hua, H. I. Huang, J. Wu, D. Hsu, M. K. Carpenter and L. A. Couture, Scalable GMP compliant suspension culture system for human ES cells, Stem Cell Res., 2012, 8, 388-402.
- R. Krawetz, J. T. Taiani, S. Liu, G. Meng, X. Li, M. S. Kallos and D. E. Rancourt, Large-scale expansion of pluripotent human embryonic stem cells in stirred-suspension bio- reactors, Tissue Eng., Part C, 2010, 16, 573-582.
- R. Olmer, A. Haase, S. Merkert, W. Cui, J. Palecek, C. Ran, A. Kirschning, T. Scheper, S. Glage, K. Miller, E. C. Curnow, E. S. Hayes and U. Martin, Long term expansion of undifferentiated human iPS and ES cells in suspension culture using a defined medium, Stem Cell Res., 2010, 5, 51-64.
- R. Olmer, A. Lange, S. Selzer, C. Kasper, A. Haverich, U. Martin and R. Zweigerdt, Suspension culture of human pluripotent stem cells in controlled, stirred bioreactors, Tissue Eng., Part C, 2012, 18, 772-784.
- H. Singh, P. Mok, T. Balakrishnan, S. N. Rahmat and R. Zweigerdt, Up-scaling single cell-inoculated suspension culture of human embryonic stem cells, Stem Cell Res., 2010, 4, 165-179. 100
- D. Steiner, H. Khaner, M. Cohen, S. Even-Ram, Y. Gil, P. Itsykson, T. Turetsky, M. Idelson, E. Aizenman, R. Ram, Y. Berman-Zaken and B. Reubinoff, Derivation, propa- gation and controlled differentiation of human embryonic stem cells in suspension, Nat. Biotechnol., 2010, 28, 361- 364. 101
- R. Zweigerdt, R. Olmer, H. Singh, A. Haverich and U. Martin, Scalable expansion of human pluripotent stem cells in suspension culture, Nat. Protoc., 2011, 6, 689-700. 102
- M. Amit, J. Chebath, V. Margulets, I. Laevsky, Y. Miropolsky, K. Shariki, M. Peri, I. Blais, G. Slutsky, M. Revel and J. Itskovitz-Eldor, Suspension culture of undifferentiated human embryonic and induced pluripo- tent stem cells, Stem Cell Rev. Rep., 2010, 6, 248-259. 103
- J. Wu, M. R. Rostami, D. P. Cadavid Olaya and E. S. Tzanakakis, Oxygen transport and stem cell aggrega- tion in stirred-suspension bioreactor cultures, PLoS One, 2014, 9, e102486. 104
- T. G. Otsuji, J. Bin, A. Yoshimura, M. Tomura, D. Tateyama, I. Minami, Y. Yoshikawa, K. Aiba, J. E. Heuser, T. Nishino, K. Hasegawa and N. Nakatsuji, A 3D sphere culture system containing functional polymers for large-scale human pluripotent stem cell production, Stem Cell Rep., 2014, 2, 734-745.
- R. Chandrasekaran and V. G. Thailambal, The influence of calcium ions, acetate and l -glycerate groups on the gellan double-helix, Carbohydr. Polym., 1990, 12, 431-442. 106
- Y. Y. Lipsitz, P. D. Tonge and P. W. Zandstra, Chemically controlled aggregation of pluripotent stem cells, Biotechnol. Bioeng., 2018, 115, 2061-2066.
- D. E. S. Nogueira, C. A. V. Rodrigues, M. S. Carvalho, C. C. Miranda, Y. Hashimura, S. Jung, B. Lee and J. M. S. Cabral, Strategies for the expansion of human induced pluripotent stem cells as aggregates in single-use Vertical-Wheel™ bioreactors, J. Biol. Eng., 2019, 13, 74. 109
- H. Wu, X. Tang, Y. Wang, N. Wang, Q. Chen, J. Xie, S. Liu, Z. Zhong, Y. Qiu, P. Situ, M. A. Zern, J. Wang, H. Chen and Y. Duan, Dextran sulfate prevents excess aggregation of human pluripotent stem cells in 3D culture by inhibit- ing ICAM1 expression coupled with down-regulating E-cadherin through activating the Wnt signaling pathway, Stem Cell Res. Ther., 2022, 13, 218.
- X. Tang, H. Wu, J. Xie, N. Wang, Q. Chen, Z. Zhong, Y. Qiu, J. Wang, X. Li, P. Situ, L. Lai, M. A. Zern, H. Chen and Y. Duan, The combination of dextran sulphate and polyvinyl alcohol prevents excess aggregation and pro- motes proliferation of pluripotent stem cells in suspen- sion culture, Cell Proliferation, 2021, 54, e13112. 111
- X. Chen, A. B. Prowse, Z. Jia, H. Tellier, T. P. Munro, P. P. Gray and M. J. Monteiro, Thermoresponsive worms for expansion and release of human embryonic stem cells, Biomacromolecules, 2014, 15, 844-855.
- X. Chen, L. Harkness, Z. Jia, A. Prowse, M. J. Monteiro and P. P. Gray, Methods for Expansion of Three- Dimensional Cultures of Human Embryonic Stem Cells Using a Thermoresponsive Polymer, Tissue Eng., Part C, 2018, 24, 146-157.
- H. J. Johnson, S. Chakraborty, R. J. Muckom, N. P. Balsara and D. V. Schaffer, A scalable and tunable thermoreversi- ble polymer for 3D human pluripotent stem cell biomanu- facturing, iScience, 2022, 25, 104971.
- R. Zhang, H. K. Mjoseng, M. A. Hoeve, N. G. Bauer, S. Pells, R. Besseling, S. Velugotla, G. Tourniaire, R. E. Kishen, Y. Tsenkina, C. Armit, C. R. Duffy, M. Helfen, F. Edenhofer, P. A. de Sousa and M. Bradley, A thermoresponsive and chemically defined hydrogel for long-term culture of human embryonic stem cells, Nat. Commun., 2013, 4, 1335. 116
- Y. Deng, S. Wei, L. Yang, W. Yang, M. S. Dargusch and Z.-G. Chen, A Novel Hydrogel Surface Grafted With Dual Functional Peptides for Sustaining Long-Term Self- Renewal of Human Induced Pluripotent Stem Cells and Manipulating Their Osteoblastic Maturation, Adv. Funct. Mater., 2018, 28, 1705546. 117
- D. Grimm, EPA plan to end animal testing splits scien- tists, Science, 2019, 365, 1231.
- X. Y. Tang, S. Wu, D. Wang, C. Chu, Y. Hong, M. Tao, H. Hu, M. Xu, X. Guo and Y. Liu, Human organoids in basic research and clinical applications, Signal Transduction Targeted Ther., 2022, 7, 168.
Cited by
Sorted by citations
Spänle, J. V., Haiber, L. M., & Seeger, B. (2026) · Frontiers in Toxicology · doi:10.3389/ftox.2026.1800157 · Cited by 1 · see citing works
- Authors
- Kawase, E., & Nakatsuji, N.
- Affiliations
- Division of Clinical Basis for ES Cell Research, Center for Human ES Cell Research, Institute for Life and Medical Sciences, Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan
- Emeritus Professor, Kyoto University, Kyoto 606-8351, Japan
- ORCID
- Kawase, Eihachiro
- Venue
- Biomaterials Science
- Publisher
- Royal Society of Chemistry (RSC)
- Published
- 2023
- Volume
- 11
- Issue
- 9
- Pages
- 2974-2987
- ISSN
- 2047-4830
- 2047-4849
- Cited by
- 20
- References
- 7
- Language
- en
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